Journal: International Journal of Molecular Sciences

Article Title: Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro

doi: 10.3390/ijms23105504

Figure Lengend Snippet: List of monoclonal antibodies used for labeling peripheral blood mononuclear cells (PBMCs) for flow cytometry. Abbreviations: FITC—Fluorescein isothiocyanate, PE—Phycoerythrin, AF647—Alexa Fluor 647, PB—Pacific Blue.

Article Snippet: CD62L , FMC46 , IgG2b , mouse , PB , MCA1076PB , BioRad , 1:5.

Techniques: Bioprocessing, Labeling, Flow Cytometry

Journal: Nature Communications

Article Title: Centrioles are frequently amplified in early B cell development but dispensable for humoral immunity

doi: 10.1038/s41467-024-53222-4

Figure Lengend Snippet:

Article Snippet: , αCD62L , Miltenyi Biotec , 130-112-836 , REA828 , 1:200.

Techniques:

Journal: Toxins

Article Title: PTX Instructs the Development of Lung-Resident Memory T Cells in Bordetella pertussis Infected Mice

doi: 10.3390/toxins13090632

Figure Lengend Snippet: The generation of Trm cells is dependent on PTX exposure during B. pertussis infection. ( A ) Representative flow cytometry of CD69 and CD103 expression of CD62L − CD44 + CD4 + T cells harvested from lung in ΔPTX, pPTX, ΔPTX + pPTX and WT groups at day 15 p.i. Frequency and absolute numbers of ( B ) CD4 + Trm and ( C ) CD8 + Trm harvested in the lung of ΔPTX, pPTX, ΔPTX+pPTX and WT groups at day 15 p.i. Results are mean ± SD for at least 3 to 5 mice/group and are representative of two independent experiments. Statistical significance of differences are shown. * p -value < 0.05; ** p -value < 0.01, *** p -value < 0.001 and **** p -value < 0.0001.

Article Snippet: T cells were enriched negatively by using Pan T kit supplemented with CD62L antibody (MiltenyiBiotec, Paris, France).

Techniques: Infection, Flow Cytometry, Expressing

Journal: Toxicology and applied pharmacology

Article Title: Resveratrol protects the ovary against chromium-toxicity by enhancing endogenous antioxidant enzymes and inhibiting metabolic clearance of estradiol

doi: 10.1016/j.taap.2016.04.016

Figure Lengend Snippet: Antibody table (source, catalog numbers, dilutions, host species and antigen sequences).

Article Snippet: Details of sources of antibodies, catalog numbers, dilutions, host species, immunogens, and homologies with rat/mouse are given in . table ft1 table-wrap mode="anchored" t5 caption a7 S·no Peptide/protein target Antigen sequence Name of antibody Manufacturer; catalog # Species raised in Dilution used 1 Cytochrome C Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of human cytochrome c Rabbit anti-cytochrome c Abcam; ab90529 Rabbit polyclonal 1:2000 2 Cleaved-caspase-3 Synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3 Rabbit anti-cleaved-caspase-3 Cell Signaling; 9661 Rabbit polyclonal 1:100 3 BCL2 N terminal amino acids 1–18 of Human Bcl-2; AGRTGYDNREIVMKYIHY Rabbit anti-Bcl-2 Abcam; ab7973 Rabbit polyclonal 1:300 4 BCL-XL Synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL Rabbit anti-BCL-xl Cell Signaling; 2762 Rabbit polyclonal 1:25 5 HIF-1α Recombinant fragment corresponding to Human HIF-1-alpha aa 329–530 Mouse anti-HIF-1α Abcam; ab8366 Mouse monoclonal 1:1000 6 GPx1 Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence) Rabbit anti-GPX-1 Abcam; ab108427 Rabbit monoclonal 1:50 7 Catalase Rat liver mitochondria Mouse anti-catalase Mitosciences; MS721-SP Mouse monoclonal 1:700 8 SOD1 Synthetic peptide corresponding to residues near the amino-terminus of human SOD1 Rabbit anti-SOD1 Cell Signaling; 2770 Rabbit polyclonal 1:25 9 SOD2 Full length protein (human) Rabbit anti-SOD-2 Abcam; ab13533 Rabbit polyclonal 1:200 10 PRDX3 Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Peroxiredoxin 3 Rabbit anti-PRDX3 Abcam; ab73349 Rabbit polyclonal 1:100 11 TXN2 Recombinant full length protein (human) Mouse anti-TXN2 Abcam; ab16857 Mouse monoclonal 1:200 12 StAR Synthetic peptide within Human StAR aa 130–180 conjugated to Keyhole Limpet Haemocyanin (KLH).

Techniques: Sequencing, Derivative Assay, Recombinant