Journal: Journal of Thoracic Disease

Article Title: The value of cell block based on fine needle aspiration for lung cancer diagnosis

doi: 10.21037/jtd.2017.07.91

Figure Lengend Snippet: Immunoreactivity for TTF1 and CD56 in small cell lung carcinoma. (A) Poorly differentiated small cell lung carcinoma in TCT (H & E, ×400); (B) SCLC in cell blocks (H & E, ×100); (C) tumor cells are positive for TTF1 (×400); (D) tumor cells are positive for CD56 (×400). SCLC, small cell lung carcinoma; TCT, ThinPrep cytology test.

Article Snippet: Antibodies to the following molecules were used: P40 (P40, 1:500, OriGene), TTF-1 (8G7G3/1, 1:200, Dako), SYN (DAK-SYNAP, 1:100, Dako), CD56 {123C3[5], 1:50, Dako}, CgA (DAK-A3, 1:100, Dako), LCA (2B11 + PD7/26, 1:100, Dako), CK5/6 (D5/16B4, 1:100, Dako) and Ki-67 (MIB-1, 1:100, Dako).

Techniques:

Journal: Biomolecules

Article Title: IL-18BP Improves Early Graft Function and Survival in Lewis–Brown Norway Rat Orthotopic Liver Transplantation Model

doi: 10.3390/biom12121801

Figure Lengend Snippet: IL-18BP overexpression reduced CD56 expression after LEW-BN OLTx. ( A ) Representative images of IHC staining for CD56 protein in livers of different groups. Scale bar, 50 μm, 25 μm. ( B ) The cumulative frequency of tissue-infiltrating CD56 + NK cells detected by IPP 6.0 in each group; error bars represent means ± SEM ( n = 3 per group); * p < 0.05; ** p < 0.01.

Article Snippet: The primary antibody was rabbit anti-CD56 monoclonal antibody (14255-1-AP, proteintech, Wuhan, China) (1:2000 dilution; 120 min at room temperature).

Techniques: Over Expression, Expressing, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: Transcriptomic profiling of pemphigus lesion infiltrating mononuclear cells reveals a distinct local immune microenvironment and novel lncRNA regulators

doi: 10.1186/s12967-022-03387-7

Figure Lengend Snippet: Identification and characterization of Immune cell subtypes in SIMC and PBMC. a the proportions of immune cells calculated by CIBERSORT, N: controls, P: pemphigus patients. b Barplot of CIBERSORT scores of SIMC in pemphigus and control skin samples. c the heatmap of deferentially expressed chemokine and chemokine receptor in SIMC between pemphigus patients and healthy controls. d Correlation heatmap of different types of immune cells. e Immunohistochemistry staining of CD56 in skin, CD56: NK cell marker. f GSEA enrichment plots of GSE37532, and GSE24574. Normalized enrichment score (NES) indicated the analysis results across gene sets. g GSEA analysis result of SIMC database

Article Snippet: The sections were blocked with 10% normal goat serum in Tris–HCl-buffered saline or horse serum in PBS for 1 h and then incubated with primary anti-NCAM1/CD56 antibodies(Clone number: EP2567Y; Abcam, Waltham, USA) at a concentration of 1:200 for 1 h at room temperature or overnight at 4℃.

Techniques: Immunohistochemistry, Staining, Marker

Journal: bioRxiv

Article Title: Upregulation of FasII underlies synergistic neuropathological and behavioral defects in a Drosophila model of myotonic dystrophy

doi: 10.1101/2024.05.26.595976

Figure Lengend Snippet: a, b Representative semi-quantitative RT-PCR of fasII in Drosophila larval (a) CNS and (b) BWM. c Quantification of a . n = 3 for both genotypes. d Quantification of b . n = 3 for both genotypes. e, f Representative slot blots of FasII in adult Drosophila (e) head and (f) muscle. g Quantification of e . n = 3 for both genotypes. h Quantification of f . n = 3 for both genotypes. i, j Representative slot blots of NCAM1 in mouse (i) head and (j) muscle. k Quantification of i . n = 3 for both genotypes. l Quantification of j . n = 3 for both genotypes. m Quantified slot blot data of NCAM1 in human frontal cortex. n = 5 for control. n = 10 for DM1 patients. n Quantitative dot blot data of NCAM1 in human transdifferentiated myotube. n = 4 for both control and DM1 patients. Histograms depicts mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Membranes were blocked in 5% Blotto (Santa Cruz Biotech, sc-2324) diluted in 1X TBS-T (10 mM Tris-HCl, 0.15 M NaCl, 0.05% Tween 20) for 1 hour at room temperature, and incubated overnight at 4°C with primary anti-NCAM1 antibody (GeneTex, GTX111684) diluted 1:10,000 in 5% Blotto.

Techniques: Quantitative RT-PCR, Dot Blot, Control, Quantitative Dot Blot