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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes
doi:
Figure Lengend Snippet: For CD28, CD5, and CD43, costimulation for Fas-independent apoptosis of HSA hi CD4 + 8 − cells in vitro fails with strong TCR ligation. HSA hi CD4 + 8 − cells from normal B6 or B6 lpr/lpr mice were cultured overnight with either a low (0.1 μg/ml; left) or high (10 μg/ml; right) concentration of anti-TCR mAb plus/minus anti-CD5, anti-CD28, or anti-CD43 mAbs (20 μg/ml). The data show mean percent differences in (Δ) apoptosis for triplicate cultures.
Article Snippet: The following mAbs were purchased from
Techniques: In Vitro, Ligation, Cell Culture, Concentration Assay
Journal: The Journal of Experimental Medicine
Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes
doi:
Figure Lengend Snippet: Costimulation-dependent apoptosis induced by TCR/CD28, TCR/CD5, or TCR/CD43 ligation in vitro is abolished by IL-7. Purified HSA hi CD4 + 8 − thymocytes from B6 lpr/lpr mice were cultured with either a low or high concentration of anti-TCR mAb plus/minus mAbs specific for CD28, CD5, or CD43; where indicated, IL-7 was added to the cultures at 100 U/ml. The data show mean levels of difference in (Δ) apoptosis for triplicate cultures. Gray bar, anti-TCR (0.1 μg/ml); dotted bar, anti-TCR (0.1 μg/ml) plus IL-7; black bar, anti-TCR (10 μg/ml).
Article Snippet: The following mAbs were purchased from
Techniques: Ligation, In Vitro, Purification, Cell Culture, Concentration Assay