Journal: Oncology Letters

Article Title: Primary pulmonary intravascular large B‑cell lymphoma: Indications from cytomorphology findings through CT‑guided puncture: A case report

doi: 10.3892/ol.2024.14792

Figure Lengend Snippet: (A) Lung tissue specimens stained with H&E stain. Within the small blood vessels, individual or small clusters of tumor cells were observed (magnification, ×100). (B) CD20 positive. (C) Bcl-6 positive. (D) Bcl-2 diffuse strong positive. (E) Multiple myeloma oncogene 1 positive. (F) p53 positive (30%+). (G) CD34 positive (vessels+). (H) C-Myc positive (30%+). (I) Ki-67 positive (>90%). (J) AE1/AE3 negative. (K) CD5 negative. (L) CD10 negative. (M) CyclinD1 negative. (N) CD30 negative. (O) Epstein-Barr encoding region negative. (B-O) All magnification, ×200.

Article Snippet: The antibody kits used included AE1/AE3 (cat. no. ZM-0069), CD20 (cat. no. ZM-0039), CD5 (cat. no. ZA-0510), CD10 (cat. no. ZM-0283), Bcl-6 (cat. no. ZM-0011), Bcl-2 (cat. no. ZA-0536), multiple myeloma oncogene 1 (MUM1; cat. no. ZA-0583), C-Myc (cat. no. ZA-0555), CyclinD1 (cat. no. ZM-0039), p53 (cat. no. ZM-0408), CD34 (cat. no. ZM-0046), CD30 (cat. no. ZM-0043) and Ki-67 (cat. no. ZM-0166), all of which were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.

Techniques: Staining

Journal: Cell reports. Medicine

Article Title: Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity.

doi: 10.1016/j.xcrm.2024.101628

Figure Lengend Snippet: Figure 1. Fratricide-resistant CD5 CART cells internalize and lose detectable CD5 (A) Schematic of the CD5.CAR and DCD5.CAR. (B) Representative histograms and MFI of surface CD5 expression in control or CD5 knocked-out (CD5KO) primary human T cells non-transduced (NT) or gammaretrovirally transduced with CD19.CAR, CD5.CAR, and DCD5.CAR constructs. Representative data from 6 individual healthy donors from 2 experiments. p values calculated using one-way ANOVA with Dunnett’s correction. Data are represented as mean ± SD. (C) CD5 protein expression in control or CD5KO primary human T cells expressing CD19.CAR, CD5.CAR, and DCD5.CAR. Representative data from 2 donors. Data are represented as mean ± SD. (D) Schema of fluorescent mScarlet-tagged CD5, fluorescent mEmerald-tagged DCD5.CAR, or bi-cistronic DCD5.CAR with CD5.mScarlet (top) and the timeline of live-imaging microscopy experiment (bottom). (E) Maximal-projection images from live-imaging fluorescent microscopy of T cells serially transduced with CD5.mScarlet and DCD5.CAR-mEmerald. mScarlet localization (top). mEmerald localization (middle). Differential interference contrast (DIC) overlay with mScarlet and mEmerald (bottom). (F) Z-slice images of mScarlet localization in T cells when CD5.mScarlet is expressed in the absence (top) or presence of DCD5.CAR (bottom).

Article Snippet: Levels of soluble CD5 were measured in thawed supernatants using Human soluble CD5 ELISA Kit (Cat # EA100720, OriGene, Rockville, MD).

Techniques: Expressing, Control, Transduction, Construct, Imaging, Microscopy

Journal: Clinical Cancer Research

Article Title: Unique Cell Surface Expression of Receptor Tyrosine Kinase ROR1 in Human B-Cell Chronic Lymphocytic Leukemia

doi: 10.1158/1078-0432.ccr-07-1823

Figure Lengend Snippet: Fig. 2. ROR1protein expression on the surface of B-CLL cells. A, flow cytometry profiles of PBMC from a representative B-CLL patient. Four-color flow cytometry was carried out with a mouse anti-human CD19 mAb conjugated to PE, a mouse anti-human CD5 mAb conjugated toAPC, gahROR1pAb or gahROR2 pAb, swine anti-goat-FITC conjugate, and propidium iodide.The histograms on the right represent staining for ROR1 (green) and ROR2 (pink) in CD5+ CD19+ B-CLL cells (top) and CD5+ CD19- Tcells (bottom).The background signal with normal goat immunoglobulin or secondary antibody alone is shownin black. B, biotinylated cell surface proteins from B-CLL cells or normal B cells were separated by SDS-PAGE. Subsequent Western blotting with gahROR1pAb and donkey anti-goat-HRP conjugate detected a single band of f120 kDa in B-CLL cells (left lane). No band was detected in normal B cells (right lane). C, B-CLL cells were incubated with gahROR1pAb for1h at 4jC. Subsequently, the cells were washed and either left at 4jC (green) or incubated for 1h at 37jC in the absence (red) or presence (blue) of 3 Amol/L phenylarsine oxide, followed by flow cytometry analysis with swine anti-goat-FITC conjugate.The background signal with secondary antibody alone is shown in black.

Article Snippet: Affinity-purified polyclonal goat anti-human ROR1 (gahROR1 pAb), polyclonal goat anti-human ROR2 antibodies (gahROR2 pAb), and polyclonal goat anti-human CD5 antibodies (gahCD5 pAb) were used as primary antibodies (R&D Systems).

Techniques: Expressing, Flow Cytometry, Staining, SDS Page, Western Blot, Incubation

Journal: iScience

Article Title: Increased peritoneal B1-like cells during acute phase of human septic peritonitis

doi: 10.1016/j.isci.2024.110133

Figure Lengend Snippet:

Article Snippet: anti-CD5-VioBlue clone REA421 , Miltenyi Biotec , cat#130-123-287; RRID: AB_2811489.

Techniques: Recombinant, Blocking Assay, Software