Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: Anti-Human CD5 (REA782) PE , Miltenyi Biotec , 130-110-990; RRID: AB_2658593.

Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy

Journal: The FASEB Journal

Article Title: A self‐organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide‐induced acute lung injury

doi: 10.1096/fj.201701506rr

Figure Lengend Snippet: Figure 2. Dynamic alterations of lung TJ and AJ, HUVEC permeability, and neutrophils after LPS administration in vivo and in vitro. E-cadherin (A, A9), VE-cadherin (B, B9), occludin (C, C0), CD11b (E, E9), and MPO (F, F9) staining and quantification of relative intensity or positive cells in 1 alveoli at 1, 3, 5, and 7 d after administration of LPS (15 mg/kg) or saline; and CD3, CD5, CD19, and CD45 staining (G) at 3 d after LPS treatment are shown. D) Double-labeling images are shown of occludin (red) with E-cadherin or VE-cadherin (green) and superimposition with DAPI (blue). H) Percentage of CD11b-, MPO-, CD3-, CD5-, CD19-, and CD45-positive cells in 1 alveoli. I) Levels of IL-6, IL-8, and TNF-a and MPO activity in the supernatants of human neutrophils at 1, 3, 5, 7, and 9 h after LPS (10 mg/ml) treatment are shown. J) HUVEC monolayer permeability, as reflected by FITC-albumin fluorescence intensity, was analyzed at 1, 3, 5, 7, and 9 h after treatment of LPS alone or LPS + neutrophil supernatants (LPS + S). Values are expressed as the means 6 SE; n = 3 (I, J); n = 10–13 alveoli from 3 mice (A2C9, E9,F9, H). Scale bars: 5 mm (D); 30 mm (A–C. E–G). N.S., no significant difference. **P , 0.01 compared with the control group, ##P , 0.01 compared with LPS group at 1 h.

Article Snippet: CD3, CD5, and CD45 antibodies were purchased from Proteintech Group (Rosemont, IL, USA).

Techniques: Permeability, In Vivo, In Vitro, Staining, Saline, Labeling, Activity Assay, Control

Journal: Research in Veterinary Science

Article Title: Relationship between rate of infection and markers of inflammation/immunity in Holy Birman cats with feline coronavirus

doi: 10.1016/j.rvsc.2014.08.009

Figure Lengend Snippet: Results recorded in Birman cats and in cats from other breeds.

Article Snippet: Immunophenotyping by flow cytometry was performed on aliquots of 50 μL of the cell suspension to identify lymphocyte subpopulations as previously described ( ) using the following panel of antibodies for feline surface antigen: 2.5 μL of mouse anti feline CD4 (specific for T helper cells, clone MCA1350, Serotec, Oxford, UK), 1 μL of mouse anti feline CD8 alpha/beta (specific for T cytotoxic cells, clone MCA1347G, Serotec, Oxford, UK), 50 μL of mouse anti feline CD5 (specific for T cells, clone MCA2038S, Serotec, Oxford, UK), and 1 μL of mouse anti canine CD21, specific for B cells, that cross reacts with feline species (clone MCA1781R, Serotec).

Techniques: