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Image Search Results
Journal: Journal of Immunology Research
Article Title: An In Vitro Comparison of Costimulatory Domains in Chimeric Antigen Receptor T Cell for Breast Cancer Treatment
doi: 10.1155/2022/2449373
Figure Lengend Snippet: Comparison of immunophenotypes between CAR.MUC1-41BB ζ and CAR.MUC1-CD28 ζ . The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3 + T cells (mean ± S.E.M from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8 + : CD4 + ratio on day 4 and day 11 after transduction is shown. Data shown are mean ± S.E.M from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with anti-CD45RO and anti-CD62L antibody (TEM: CD45RO + CD62L − , TCM: CD45RO + CD62L + , TN: CD45RO − CD62L + , and TE: CD45RO − CD62L − ). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as mean ± S.E.M ( n = 6).
Article Snippet: For cell surface marker analysis, the following antibodies were used: CD56-PE (HCD56/Cat#130-114-551), CD4-FITC (OKT4/130-114-531), CD8-APC (SK1/130-110-679),
Techniques: Comparison, Transduction, Flow Cytometry, Staining