Journal: Human immunology

Article Title: Increased T Cell Immunosenescence and Accelerated Maturation Phenotypes in Older Kidney Transplant Recipients

doi: 10.1016/j.humimm.2018.06.006

Figure Lengend Snippet: Frequency of maturation subtypes by patient age. PBMC were analyzed for naive (CCR7+/CD45RA+), effector memory (EM) (CCR7-/CD45RA-), and terminally differentiated effector memory RA+ (TMRA) (CCR7-/CD45RA+) T cell content, or CD4+KLRG1+, CD8+ KLRG1+, and NKT KLRG1+ cells, or CD8+ CD57+CD28- and CD8+CD57+KLRG1+ T cells, as indicated, expressed as a percentage of the total number of CD8+ T cells or NKT cells Each dot corresponds to a sample; bars indicate median. *** indicates p<0.001, ** indicates p<0.01, by nonparametric test, and * indicates p<0.05 by nonparametric test. A. CD8+ maturation subtypes by patient age. B. CD4+ maturation subtypes by patient age. C. Immune senescence as measured by KLRG1 expression by patient age. D. T cell activation and immune senescence as measured by CD57+ and CD28- and KLRG1+ by patient age.

Article Snippet: Naïve cells were defined as CCR7+/CD45RA+; effector memory as CCR7-/CD45RA-; and terminally differentiated as CCR7-/CD45RA+ (antibodies obtained from BD Biosciences or Biolegend) ( Supplemental Figure ).

Techniques: Expressing, Activation Assay

Journal: Scientific Reports

Article Title: The tetraspanin CD151 marks a unique population of activated human T cells

doi: 10.1038/s41598-020-72719-8

Figure Lengend Snippet: Increased CD4+CD151+ T cell frequencies in the peripheral blood of HIV-1 patients on fully suppressive ART. ( a ) Beeswarm plots describing the frequencies of CD4+CD151+ T cells from healthy donors (controls), and HIV-seropositive individuals on ART (HIV/ART). Red lines indicate median frequencies. The 95% interval for the normal distribution of CD151+ T cell frequencies in healthy donors (95% reference range) was calculated and is indicated as a red dotted line. ( b ) The bar graphs show the distribution of individuals based on their CD4+CD151+ T cell frequencies into groups with < 10% CD4+CD151+ T cells (blue), between 10%-30% (green) and > 30% (red), in healthy controls and HIV/ART patients. The frequency of CD151- and CD151+Cells in healthy and HIV/ART patients with preformed Granzyme B for ( c ) CD4+ T cells and ( d ) CD8+ T cells. ( e ) CD151+ T cell expression in relation to CD28 or CD57 expression on CD4+ T cells from HIV/ART patients. Numbers indicate the percentage of cells within each quadrant. ( f ) Relative contribution of the CD28− T cell population to the total CD151+ T cell population in healthy controls and HIV/ART patients with CD4+ T cells counts between 300 and 600 or < 300 cells/mm 3 . ( g ) Comparison of the frequency of CD4+CD151+ T cells as a function of the memory differentiation state between healthy control individuals (open symbols) and HIV/ART patients (closed symbols). T cell subsets were defined by patterns of CD45RA and CCR7 expression: Naïve T cells (T N : CD45RA+CCR7+), central memory T cells (T CM : CD45RA–CCR7+), effector memory T cells (T EM : CD45RA – CCR7 – ) and T effector memory RA-positive cells (T EMRA : CD45RA+CCR7–). Median values are depicted by red lines. ( h ) CFSE-labeled PBMCs from a HIV-seropositive individual were stimulated with overlapping Gag peptides for 4 days and increased CD151 expression on proliferating Gag-specific T cells was quantified using flow cytometric analysis. Unstimulated cells served as the negative control.

Article Snippet: Antibodies recognizing CD45RA-APC (HI100, BD Biosciences) or CD45RA-Alexa Fluor 700 (HI100, BD Biosciences) and CCR7-Percpcy5.5 (150503, BD Biosciences) were used to define naïve T cells (T N : CD45RA + CCR7 + ), central memory T cells (T CM : CD45RA – CCR7 + ), effector memory T cells (T EM : CD45RA – CCR7 – ) and T effector memory RA-positive cells (T EMRA : CD45RA + CCR7 – ) as previously reported .

Techniques: Expressing, Labeling, Negative Control