Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: CyTOF antibody panel

Article Snippet: Anti-Human CD45RA (HI100)-143Nd , Fluidigm , Cat# 3143006B; RRID:AB_2651156.

Techniques:

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet:

Article Snippet: Anti-Human CD45RA (HI100)-143Nd , Fluidigm , Cat# 3143006B; RRID:AB_2651156.

Techniques: Cell Analysis, Purification, Virus, Recombinant, Blocking Assay, Antibody Labeling, Transfection, Software

Journal: Current Oncology

Article Title: Inflammation and Fibrosis in Patients with Non-Cirrhotic Hepatitis B Virus-Associated Hepatocellular Carcinoma: Impact on Prognosis after Hepatectomy and Mechanisms Involved

doi: 10.3390/curroncol30010016

Figure Lengend Snippet: CyTOF panel.

Article Snippet: CD45RA , HI100 , 170Er , Fluidigm.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions

doi: 10.3390/ijms22084158

Figure Lengend Snippet: CCL19-S6 649P1 is a versatile tool to stain CCR7-expressing primary human and mouse T cell sub-populations. Primary human peripheral blood CD3-sorted T cells ( a , b ) and mouse splenic T cells ( c ) were stained with 50 nM CCL19-S6 649P1 for 20 min at 22 °C together with either anti-huCD4-FITC, anti-huCD45RA-FITC, anti-huCD45RO-FITC, anti-huCCR7-APC, anti-huCCR7-PacificBlue, anti-muCD3-PE, or anti-muCCR7-APC conjugated antibodies, respectively, and analyzed by flow cytometry. One ( a ) out of the two ( b ) human donors, or three ( c ) independent experiments with comparable results are shown.

Article Snippet: For flow cytometry, the following antibodies were used: huCCR7-PacificBlue (Biolegend, San Diego, CA, USA, #353210), huCCR7-APC (R&D Systems, #FAB197A), huCD3-PacificBlue (Biolegend, San Diego, CA, USA, #300417), huCD4-FITC (Bio-Rad, Hercules, CA, USA, #MCA1267F), huCD45RA-FITC (Bio-Rad, Hercules, CA, USA, #MCA88F), huCD45RO-FITC (Bio-Rad, Hercules, CA, USA, #MCA461F), muCD3ε-PE (Biolegend, San Diego, CA, USA, #100307), muCCR7-APC (ThermoFisher, Waltham, MA, USA, #17-1971-82) and rat IgG2a kappa isotype control (ThermoFisher, Waltham, MA, USA, #17-4321-81).

Techniques: Staining, Expressing, Flow Cytometry

Journal: Cell reports

Article Title: The aryl hydrocarbon receptor cell intrinsically promotes resident memory CD8 + T cell differentiation and function

doi: 10.1016/j.celrep.2022.111963

Figure Lengend Snippet: (A–C) Human peripheral blood (PBMC) vs. IEL CD8 + T cells were analyzed via flow cytometry. Staining of CD45RA and CD45RO (top), CD103 and CD69 (bottom) (A), as well as T-BET and AHR (B) in tissue fractions as indicated in the figure. Quantification of AHR protein levels (gMFI) in human PBMC, LPL, and IEL CD8 + T cells (C). Data are compiled from three independent experiments and shown as mean ± SEM (n = 4 replicates per group). (D–G) Human naive CD8 + T cells were isolated from PBMCs and then subjected to in vitro T RM -like differentiation culture conditions. The cells were given differentiation cytokines and treated with DMSO (control), FICZ, or CH223191 on day 2. The assay was collected on day 4, and flow cytometry quantification of CD103 expression was performed (D). Data are compiled from three independent experiments and are shown as mean ± SEM (n = 4 replicates per group). RNA was isolated for qRT-PCR expression analysis of AHR (E), AHR direct target gene AHRR (F), and GZMB (G). Data are shown as mean ± SEM (n = 3 technical replicates per group). Data are representative of three independent experiments.

Article Snippet: Anti-human CD45RA – PerCP-Cyanine5.5 (clone HI100) , TONBO biosciences , Cat# 65-0458-T100; RRID: AB_2621896.

Techniques: Flow Cytometry, Staining, Isolation, In Vitro, Control, Expressing, Quantitative RT-PCR

Journal: Cell reports

Article Title: The aryl hydrocarbon receptor cell intrinsically promotes resident memory CD8 + T cell differentiation and function

doi: 10.1016/j.celrep.2022.111963

Figure Lengend Snippet:

Article Snippet: Anti-human CD45RA – PerCP-Cyanine5.5 (clone HI100) , TONBO biosciences , Cat# 65-0458-T100; RRID: AB_2621896.

Techniques: Virus, Isolation, Recombinant, SYBR Green Assay, DNA Library Preparation, Reverse Transcription, Staining, Transgenic Assay, Plasmid Preparation, Software