Journal: Arthritis Research & Therapy

Article Title: Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells

doi: 10.1186/ar2459

Figure Lengend Snippet: Surface epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs). (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitor cells (BM-MPCs). T-MPCs are shown in the top two rows of panels, and BM-MPCs are shown in the bottom two rows of panels. Epitopes were detected using fluorescently labeled secondary antibodies (red). Nuclei were stained with DAPI (blue). Both cell populations were negative for CD14, CD34, and CD45 and positive for CD29, CD44, and CD105. Bars = 20 μm. (b) Flow cytometric analysis of T-MPCs and BM-MPCs. CD14, CD34, CD45, CD105, CD73, and CD90 were detected by fluorescently conjugated antibodies. The level of expression of each epitope is expressed as the mean fluorescence intensity ± standard deviation (n = 3). (c) Representative flow cytometry histogram. Control represents fluorescence due to the isotypic control. DAPI, 4',6-diamidino-2-phenylindole dihydrochloride.

Article Snippet: For flow cytometry, T-MPCs (>1 × 10 5 cells) were washed and resuspended in PBS + 0.1% FBS (PF) containing saturating concentrations (1:100 dilution) of the following conjugated mouse IgG 1,κ anti-human monoclonal antibodies (BD Biosciences): HLA-A, B, C-phycoerythrin (PE) (MHC I), HLA-DR, DP, DQ-fluorescein isothiocyanate (FITC) (MHC II), CD45-FITC, CD14-PE, CD31-PE, CD34-PE, CD73-PE, CD90-FITC, CD105-PE as well as IFN-γR1-PE (R&D Systems, Inc., Minneapolis, MN, USA) for 1 hour at 4°C.

Techniques: Derivative Assay, Immunofluorescence, Labeling, Staining, Expressing, Fluorescence, Standard Deviation, Flow Cytometry, Control