Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 1. EPC morphology and gene expression. (A) Appearance of a freshly isolated EPC colony at passage 0 on day 9 of culture. (B) Appearance of EPCs in culture at passage 2, (C–F) EPCs express the endothelial cell specific markers CD31 (C, red, DAPI blue) and CD144 (D, green, DAPI blue) and pluripotency associated markers NANOG (E, red) and REX-1 (F, red, DAPI blue). Images A-B at 50X magnification, images C, D, E, F at 100X magnification.

Article Snippet: Primary EPCs were stained using specific antibodies against CD31 (Cat. no. BBA7, R&D Systems, Abingdon, UK), CD144 (Cat. no. MAB9381, R&D Systems) and REX1 (Cat. no. AF3598, R&D Systems), or by using ULEX (Cat. no. RL-1062-2, Vector Laboratories) or DAPI (Cat. no. H-1200-10, Vector Laboratories) at the dilution recommended by the manufacturer.

Techniques: Gene Expression, Isolation

Journal: Molecular medicine reports

Article Title: Swimming training promotes angiogenesis of endothelial progenitor cells by upregulating IGF1 expression and activating the PI3K/AKT pathway in type 2 diabetic rats.

doi: 10.3892/mmr.2024.13361

Figure Lengend Snippet: Figure 2. Morphology and identification of EPCs. (A) Characterization of EPCs from rats grown in vitro (magnification, x100). (B) Immunofluorescence staining with Dil‑ac‑LDL and/or FITC‑UEA‑I (scale bar, 20 µm). (C) Fluorescence microscopy detection of phenotypes CD31, CD34 and CD133 (scale bar, 20 µm). EPC, endothelial progenitor cell; Dil‑ac‑LDL, 1,1'‑dioctadecyl‑3,3,3', 3'‑tetramethylindocarbocyanine perchlorate‑labeled acetylated low‑density lipoprotein; FITC‑UEA‑I, fluorescein isothiocyanate‑conjugated Ulex europaeus agglutinin‑I.

Article Snippet: The primary antibodies were: CD31 (1:100; Proteintech Group; cat. no. CL488‐66065), CD34 (1:100; BIOSS; cat. no. bsm‐41197M) and CD133 (1:100; Proteintech Group; cat. no. 18470‐1‐AP).

Techniques: In Vitro, Immunofluorescence, Staining, Fluorescence, Microscopy

Journal: International journal of cardiology

Article Title: Enhanced external counterpulsation inhibits endothelial apoptosis via modulation of BIRC2 and Apaf-1 genes in porcine hypercholesterolemia.

doi: 10.1016/j.ijcard.2013.11.033

Figure Lengend Snippet: Fig. 2. Isolation and identification of vascular endothelial cells from the aortic endothelium with collagenase. Representative photomicrographs (×400) of isolated cells treated histocytochemically with CD31 antibody and incubated with DiI-Ac-LDL. Cells with cytomembrane and cytoplasmic staining of amber color indicate vascular endothelial cells (black arrow), and cells with red fluorescence were identified as viable vascular endothelial cells (white arrow), which consisted of the majority of total treated cells, indicating a successful en- dothelial cell isolation from aortic endothelium.

Article Snippet: Then mouse polyclonal CD31 antibody (at 1:100 dilution; Boster Biological Technology, Inc, Wuhan, China) was applied and incubated at 37 °C for 1 h, then stained with SABC, enhanced by DAB, dehydrated in a graded ethanol series, and followed by dimethylbenzene treatment to improve transparency, and finally mounted with neutral gum.

Techniques: Isolation, Incubation, Staining, Cell Isolation