Journal: Blood Cancer Journal

Article Title: A superantigen-based MHC class II-targeted cancer immunotherapy for the treatment of acute myeloid leukemia

doi: 10.1038/s41408-025-01391-w

Figure Lengend Snippet: A Flow cytometry of various AML cell lines showing CD33 cell surface expression with median fluorescence intensity (MFI) quantification. Significance was determined by Mann-Whitney test, ** P < 0.01. B Western blot for anti-6X His tagged recombinant proteins. C ELISA for M2T-CD33 binding to various MHCII alleles, including human, mouse, and dog. IgG1 Fc Knob-in-Hole as a control for MHCII alleles constructed with IgG1 Fc stalk. BSA as negative control. D Flow cytometry of Daudi and Jurkat cells showing TCR and MHCII (HLA-DR) expression, M2T-CD33 binding, and M2T-CD33 double mutant (DM) binding. E Flow cytometry of human PBMCs (healthy donor #1) gating on CD3 or CD19 and then comparing binding of CD33 vs. M2T-CD33. F Flow cytometry of mouse splenocytes gating on CD3 or CD19 and then comparing binding of CD33 vs. M2T-CD33.

Article Snippet: Anti-Mouse CD3 PE (Cat #FAB4841P) was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Flow Cytometry, Expressing, Fluorescence, MANN-WHITNEY, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay, Control, Construct, Negative Control, Mutagenesis

Journal: Digestive Diseases and Sciences

Article Title: Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer

doi: 10.1007/s10620-024-08536-0

Figure Lengend Snippet: Downregulation of peripheral blood NK cell-activating receptors NKG2D and NKp30 and cytokine factors IFN-γ and TNF-α in gastric cancer. A – C NK cell gating strategy. D and E Flow cytometry analysis comparing the expression of NK cell subsets CD3−/CD56+ and CD3−/CD16+ in GC and HD peripheral blood. F – H Flow cytometry analysis comparing the expression of NK cell receptors NKG2D, NKp30, and NKp46 in GC and HD peripheral blood. I and J ELISA detection of cytokine IFN-γ and TNF-α expression in GC (n = 16) and HD (n = 16) plasma. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant

Article Snippet: Single-cell suspensions were stained using the following antibodies: anti-human CD3 PE (BioGems, USA), anti-human CD16 PE-Cy7 (BioGems), anti-human CD56 APC (BioGems), anti-human NKG2D (CD314) PE (BioGems), anti-human NKp30(CD337) PE-Cy7 (BD, USA), anti-human NKp46(CD335) APC (BD), and PD-L1 PE (CST, USA).

Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Digestive Diseases and Sciences

Article Title: Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer

doi: 10.1007/s10620-024-08536-0

Figure Lengend Snippet: Correlation of plasma exosomal miR-552-5p with NK cell phenotypes and activated receptors in gastric cancer. A and B Negative correlation between plasma exosomal miR-552-5p expression and the distribution of CD3−/CD56+ and CD3−/CD16+ subpopulations in peripheral blood NK cells of gastric cancer patients. C – E Negative correlation between plasma exosomal miR-552-5p expression and the expression of peripheral blood NK cell-activating receptors NKG2D, NKp30, and NKp46 in gastric cancer

Article Snippet: Single-cell suspensions were stained using the following antibodies: anti-human CD3 PE (BioGems, USA), anti-human CD16 PE-Cy7 (BioGems), anti-human CD56 APC (BioGems), anti-human NKG2D (CD314) PE (BioGems), anti-human NKp30(CD337) PE-Cy7 (BD, USA), anti-human NKp46(CD335) APC (BD), and PD-L1 PE (CST, USA).

Techniques: Clinical Proteomics, Expressing

Journal: Integrative Cancer Therapies

Article Title: Antitumor and Immunomodulatory Activities of Ganoderma lucidum Polysaccharides in Glioma-Bearing Rats

doi: 10.1177/1534735418762537

Figure Lengend Snippet: Cell proliferation detected by EdU incorporation (n = 4). Twenty-one days after tumor implantation, rats were injected intraperitoneally with 5 mg/kg of EdU and spleen cells were separated 48 hours after injection. After staining by Apollo 488 azide and CD3-PE antigen, the cells were washed twice with phosphate-buffered saline and 0.05% saponin before flow cytometry analysis. The numbers in the upper right quadrant indicate the percentages of EdU+ cells among CD3+ lymphocytes (** P < .01, * P < .05).

Article Snippet: T-lymphocyte proliferation was tested by flow cytometry analysis by CD3-PE antigen labeling combined with 5-ethynyl-29-deoxyuridine (EdU; RiboBio, Guangzhou, China) incorporation.

Techniques: Tumor Implantation, Injection, Staining, Flow Cytometry