Journal: Journal of Neurology

Article Title: Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis

doi: 10.1007/s00415-023-11779-y

Figure Lengend Snippet: Immunohistochemistry and double immunofluorescence staining of HIV-IBM and sIBM. HIV-IBM cases showed no KLRG1 + or CD57 + cells in contrast to the sIBM cases ( b1–4 , c1–4 ). Many CD8 + /CD27 + and CD3 + /PD1 + cells were detectable in both groups ( a1–4 , d1–4 ). The ligands showed a weak expression for PD-L1 ( e1 , e3 ) and a non-specific staining pattern involving the endomysial connective tissue and myocyte sarcolemma cells for PD-L2 ( e2 , e4 )

Article Snippet: The following Taq-Man Gene Expression Assays from Life Technologies/ThermoFisher were used: B3GAT1 (CD57), Hs01024500_m1; CD244, Hs00175569_m1; CD27, Hs00609654_g1; KLRG1 , Hs00195153_m1; PD1 , Hs01550088_m1; PDL1 , Hs00204257_m1; PDL2 , Hs00228839_m1; PGK1 , Hs99999906_m1; TBX21 , Hs00894392_m1.

Techniques: Immunohistochemistry, Double Immunofluorescence Staining, Expressing, Staining

Journal: Journal of Neurology

Article Title: Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis

doi: 10.1007/s00415-023-11779-y

Figure Lengend Snippet: Quantitative immunohistochemistry for CD27, KLRG1, CD57 and PD1: comparison of HIV-IBM (green) and sIBM (blue). The average cell counts from ten high power fields (HPF) are shown in a boxplot. Statistical calculations were performed for the Mann–Whitney U test. The level of significance was set at 1% (**). KLRG1 + cells were undetectable in the HIV-IBM group, but showed a significantly higher expression in the sIBM group. There was no significant difference in the number of CD27-, CD57- and PD1-positive cells between patients with HIV-IBM and sIBM

Article Snippet: The following Taq-Man Gene Expression Assays from Life Technologies/ThermoFisher were used: B3GAT1 (CD57), Hs01024500_m1; CD244, Hs00175569_m1; CD27, Hs00609654_g1; KLRG1 , Hs00195153_m1; PD1 , Hs01550088_m1; PDL1 , Hs00204257_m1; PDL2 , Hs00228839_m1; PGK1 , Hs99999906_m1; TBX21 , Hs00894392_m1.

Techniques: Immunohistochemistry, MANN-WHITNEY, Expressing

Journal: Journal of Neurology

Article Title: Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis

doi: 10.1007/s00415-023-11779-y

Figure Lengend Snippet: Quantitative PCR for CD27, CD57, CD244, KLRG1, TBX21, PD1, PDL1, PDL2: comparison of HIV-IBM (green), sIBM (blue) and NDC (grey). Delta CT values are shown in a boxplot on the inverse axis. Statistical calculations were performed for the Kruskal–Wallis test with Bonferroni–Dunn correction to analyze the quantitative differences of mRNA transcripts. The level of significance was set at 5% (*) and 1% (**). All markers were shown to be significantly upregulated at gene expression levels compared to the NDC group, with no significant difference between the IBM subgroups

Article Snippet: The following Taq-Man Gene Expression Assays from Life Technologies/ThermoFisher were used: B3GAT1 (CD57), Hs01024500_m1; CD244, Hs00175569_m1; CD27, Hs00609654_g1; KLRG1 , Hs00195153_m1; PD1 , Hs01550088_m1; PDL1 , Hs00204257_m1; PDL2 , Hs00228839_m1; PGK1 , Hs99999906_m1; TBX21 , Hs00894392_m1.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Cell Reports Methods

Article Title: Development of an HIV reporter virus that identifies latently infected CD4 + T cells

doi: 10.1016/j.crmeth.2022.100238

Figure Lengend Snippet:

Article Snippet: Anti-human CD27 (REA499) 155Gd, coupled in house , Miltenyi Biotec , Cat#: 130122295, RRID: AB_2801876.

Techniques: Virus, Plasmid Preparation, Recombinant, Cell Isolation, Staining, Software

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Phenotypic analysis of circulating Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and in healthy subjects. Distribution of ex vivo memory subsets of Vδ1+ ( A ) and Vδ2+ ( C ) T lymphocytes based on the expression of CD27 and CD45RA in hospitalized and recovered COVID-19 patients, compared to healthy donors. Median is shown. Healthy donors were represented as triangle, COVID-19 patients as circle and COVID-19 recovered as rhombus. Representative dot-plots showing Vδ1+ ( B ) and Vδ2+ ( D ) T lymphocyte memory subset distribution in hospitalized, recovered, and healthy subjects. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: To assess the ex vivo frequency, phenotype, and exhaustion-marker expression of γδ T lymphocytes, PBMCs were stained with specific fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), PE-conjugate anti-human Vδ1 (clone REA173), PE-Vio770-conjugate anti-human Vδ2 (clone REA771), FITC-conjugate anti-human TIM3 (clone F38-2E2), APC-conjugate anti-human PD-1 (clone PD1.3.1.3), PE-Vio615-conjugate anti-human CD27 (clone REA499), and VioBlue-conjugated anti-human CD45RA (clone REA1047) (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Ex Vivo, Expressing

Journal: Human Genomics

Article Title: Immune dysregulation associated with co-occurring germline CBL and SH2B3 variants

doi: 10.1186/s40246-022-00414-y

Figure Lengend Snippet: Results of immunological characterization of the studied family. DNT, γ + δ + T and effector CD8 + memory cells are increased in II.2, III.2 and III.3; II.2 shows a B-cell memory deficiency

Article Snippet: On peripheral blood, after red blood cell lysis with ammonium chloride, the following antibodies were employed: CD3 PerCP (clone BW264/56, MiltenyiBiotec, BergischGladbach, Germany), CD4 APC (clone OKT4, Becton Dickinson, Franklin Lakes, NJ, USA), CD8 PE-Cy7 (clone RPA-T8, Becton Dickinson, USA), TCR alpha–beta APC (clone T10B9, Becton Dickinson), TCR gamma-delta FITC (11F3, MiltenyiBiotec, DE), CD45RA APC-H7 (clone T6D11, MiltenyiBiotec, DE), CCR7 PE (clone 3D12, Ebioscience, San Diego, CA, USA), CD127 PE-CY7 (clone eBioRDR5, eBiosciences), CD16 PE (clone 3G8), CD56 PE (clone NCAM16.2), CD19 PE-CY7 (clone SJ25C1, Becton Dickinson), CD27 FITC (clone M-T271, Becton Dickinson), CXCR5 (clone J252D4 Biolegend), CD31 (cloneWM59, Miltenyi Biotec, DE).

Techniques: Expressing

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Antibodies and Clones Used for Immunohistochemistry

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Clone Assay

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Patient Demographics and Clinical Characteristics

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques:

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Classification of Tumors According to the 2016 WHO Classification of Central Nervous System Tumors, Revised 4th Edition

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques:

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Pathologic Characteristics of CD70+ (2–3) Gliomas

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques:

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Histologic and immunophenotypic features of CD70+ epithelioid glioblastoma. Three cases of E-GBM showing characteristic round cell morphology and well-delineated cell borders (A–C). E-GBMs demonstrated a characteristic immunophenotype, with patchy expression of GFAP (D) and cytokeratins (E), retained nuclear expression of INI1 (F), and negative IDH R132H (not shown). Strong membranous immunoreactivity for CD70 (1:500, clone MAB2738, R&D Systems) (G–I).

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Expressing

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: CD70 expression in gliosarcoma. Illustrated are two separate cases of secondary gliosarcoma (A, E) showing the characteristic biphasic features by light microscopy: gliomatous areas highlighted by GFAP (B, F), and a dense peri-cellular reticulin meshwork in the sarcomatous portions of the tumor (C, G). Most gliosarcomas showed expression of CD70 restricted to the mesenchymal/sarcomatous (reticulin-rich/GFAP-poor) portions of the tumor (D, H, I). Half of the gliosarcomas with moderate-to-strong CD70 expression (5/10) also showed focal cytokeratin (CAM5.2) immunoreactivity (not shown).

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Expressing, Light Microscopy

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Staining patterns of CD70 in high-grade gliomas. A prominent Golgi pattern of staining was seen in epithelioid tumor cells (black arrowhead) (A). Isolated dot-like cytoplasmic staining with an absence of membrane staining (B). Cytoplasmic staining in tumor cells was a frequent finding (C). This proved challenging in cases with high-background, but comparison with endothelial expression (black arrowhead) resolved this dilemma. Accordingly, staining of tumor microvasculature (including the entity-defining microvascular proliferation) was a consistent finding (D); only cytoplasmic staining was seen (E) and this was observed in both treatment-naïve and post-radiotherapy tissues. Neurons (black arrowhead) occasionally showed staining of the soma and typically occurred in states of injury (eg perineuronal satellitosis [white arrowhead] and ischemia) (F). CD70 expression in a diffuse midline glioma, H3 K27M mutant (G: anti-CD70; H: anti-H3 K27M-mutant) and an anaplastic oligodendroglioma, IDH-mutant and 1p/19q co-deleted (I: anti-CD70; J: anti-IDH R132H).

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Staining, Isolation, Membrane, Comparison, Expressing, Mutagenesis

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Quantitative analysis of high-grade gliomas with moderate-to-strong (2–3) CD70 expression. Analysis of diagnostic groups showed disproportionate expression in IDH-wildtype glioblastoma (A). Gliosarcoma and E-GBM showed significantly higher expression of CD70 when compared to IDH-wildtype glioblastoma with classic histology (GBIDHWT*), as well as other high-grade diffuse gliomas (*p < 0.05) (B). Evaluation of a small validation dataset (Vital) showed higher CD70 mRNA in gliosarcomas compared to glioblastomas (C). TCGA data showed significantly higher CD70 mRNA expression in mesenchymal tumors compared to other gene expression-based profiles (**p < 0.01) (D).

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Expressing, Diagnostic Assay

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Clinical Characteristics and Treatment History of Cases With Moderate-to-Strong CD70 Expression

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Expressing, Adjuvant

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Kaplan–Meier estimation of survival probability. Gliosarcomas showing moderate-to-strong expression of CD70 (>5% of tumor cells) showed an association with prolonged post-radiation survival compared to gliosarcomas with negative or weak expression, as measured from the last date radiation to death (p < 0.05) (A). Comparison of overall survival in the same groups showed a trend towards significance (p = 0.062) (B). Survival estimation generated from TCGA data in non-G-CIMP tumors showed significantly shorter overall survival in recurrent tumors with low CD70 mRNA expression compared to tumors with high expression (p < 0.01) (C).

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques: Expressing, Comparison, Generated

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma

doi: 10.1093/jnen/nlx051

Figure Lengend Snippet: Increased TAM in CD70+ gliomas. Post-radiotherapy CD70+ high-grade gliomas showed a significant increase in intratumoral CD163+ macrophages when compared to CD70- tumors (*p < 0.05).

Article Snippet: While a subset of activated lymphocytes express CD70 under normal conditions, absence of staining in adjacent immune cells provided an internal control. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody Clone Clonality Dilution Source Localization IDH1 R132H H09 Monoclonal Pre-dilute Dianova Cytoplasmic ATRX HPA001906 † Polyclonal 1:200 Sigma-Aldrich Nuclear p53 DO-7 Monoclonal 1:1000 Dako Nuclear H3K27M ABE419 † Polyclonal 1:400 EMD Millipore Nuclear EGFR 31G7 Monoclonal 1:50 Invitrogen Membranous/cytoplasmic INI1 MRQ-27 Monoclonal Pre-dilute Roche Nuclear CD70 (CD27L) MAB2738 Monoclonal 1:500 R&D Systems Membranous/cytoplasmic * CD27 EPR8569 Monoclonal 1:800 Abcam Membranous CD68 KP-1 Monoclonal Pre-dilute Roche Membranous/cytoplasmic CD163 MRQ-26 Monoclonal Pre-dilute Roche Membranous/cytoplasmic HLA-DR TAL 1B5 Monoclonal 1:200 Dako Membranous/cytoplasmic GFAP EP672Y Monoclonal Pre-dilute Roche Cytoplasmic CAM5.2 CAM5.2 Monoclonal Pre-dilute Roche Cytoplasmic Open in a separate window * See figures for observed staining patterns.

Techniques:

Journal: Nature Communications

Article Title: Fasting alters the gut microbiome reducing blood pressure and body weight in metabolic syndrome patients

doi: 10.1038/s41467-021-22097-0

Figure Lengend Snippet: Antibodies used for the flow cytometry analysis.

Article Snippet: a-CD27 PerCP-Vio700 , Miltenyi , AB_2660841 , 1:10.

Techniques: Flow Cytometry