Journal: Toxicology reports

Article Title: Silver nanoparticles induces apoptosis of cancer stem cells in head and neck cancer.

doi: 10.1016/j.toxrep.2023.11.008

Figure Lengend Snippet: Fig. 4. CSC isolated from Cal33 characterization by Flow Cytometry. Population 1 and Single cell dot plots show the gating strategy for events of interest and eliminating doublets and triplets. Untreated is a negative control for CD44+PE and CD24+APC staining. Before CSC isolation shows 55% of CD44+ and CD24 low

Article Snippet: FITC Annexin V Apoptosis Detection Kit (Cat no. 640914) was purchased from BD Bioscience, MagCellect Streptavidin Ferrofluid (Cat no. MAG999B), CD24 Biotinylated Antibody (Cat no. FAB52471B), Human CD24 APC-conjugated Antibody (Cat no. FAB5247A) and Human CD44 PE-conjugated Antibody (Cat no. FAB8547P) from R&D Systems Minneapolis, Minnesota, United States.

Techniques: Isolation, Flow Cytometry, Negative Control, Staining

Journal: Stem Cell Research & Therapy

Article Title: Lumican from DESC spheroids enhances odontoblastic differentiation of SCAPs

doi: 10.1186/s13287-025-04849-7

Figure Lengend Snippet: Characterization of SCAPs and DESCs. a Microscopic image of the apical buds from the lower incisors of 5-7-day-old C57BL/6 mice. b Primary mixed cell population. c Purified SCAPs exhibiting spindle-shaped morphology. d Multidirectional differentiation potential of SCAPs. Alcian Blue, Alizarin Red, and Oil Red O staining confirmed glycosaminoglycan deposition, mineralized nodule formation, and lipid droplet accumulation, indicative of chondrogenic, osteogenic, and adipogenic differentiation, respectively. e , f Flow cytometry analysis revealed CD24, CD133, and CD90 positivity, and CD45 negativity in SCAPs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3. g Purified DESCs displaying cobblestone-like morphology. h Immunofluorescence imaging demonstrated positive K14 expression in DESCs (K14, green; DAPI, blue). i , j Flow cytometry analysis revealed CD29 and CD49f positivity, and CD90 negativity in DESCs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3

Article Snippet: For SCAP analysis, cells were labeled with allophycocyanin (APC)-conjugated CD24 antibody (APC-65121, Proteintech, China, 1:300) as well as FITC-conjugated CD133 (11-1331-82, Thermo Fisher Scientific, USA, 1:500), CD90, and CD45 (MCD4501, Thermo Fisher Scientific, USA, 1:500) antibodies.

Techniques: Purification, Staining, Flow Cytometry, Immunofluorescence, Imaging, Expressing