Journal: bioRxiv

Article Title: Differential Expression of the T cell Inhibitor TIGIT in Glioblastoma and Multiple Sclerosis

doi: 10.1101/591131

Figure Lengend Snippet: (A) TIGIT + , CD226 + and CD155 + infiltrates in tumor tissue from GBM patients and chronic active lesions from MS patients. (B) CD155 expression in tumor cells and cortical neurons. (C) Quantification of TIGIT + , CD226 + and CD155 + infiltrates in GBM tumor tissue and MS lesions. Statistical significance was assessed by unpaired Student’s t tests with a p-value threshold of 0.05. ns = non-significant. Scale bars = 40 μm.

Article Snippet: Serial sections were stained with primary antibodies against CD3 (Dako A 40452, 1:200), TIGIT (Santa Cruz sc-103349, 1:800), CD226 (Novus Biologicals NBP1-85001, 1:50), and CD155 (Bioss Inc. bs-2525R, 1:750), and processed with the appropriate biotinylated secondary antibodies (Vector Laboratories, Burlingame CA) and avidin-biotin staining kit with diaminobenzidine as chromogen.

Techniques: Expressing

Journal: bioRxiv

Article Title: Differential Expression of the T cell Inhibitor TIGIT in Glioblastoma and Multiple Sclerosis

doi: 10.1101/591131

Figure Lengend Snippet: (A) PD-1 + and PD-L1 + infiltrates in tumor tissue from GBM patients and chronic active lesions from MS patients. (B) Quantification of PD-1 + and PD-L1 + infiltrates in GBM tumor tissue and MS lesions. (C) Frequency of TIGIT + and CD226 + lymphocytes in the perivascular space and deep parenchyma. Statistical significance was assessed by unpaired Student’s t tests with a p-value threshold of 0.05. ns = not significant. Scale bar = 40 μm.

Article Snippet: Serial sections were stained with primary antibodies against CD3 (Dako A 40452, 1:200), TIGIT (Santa Cruz sc-103349, 1:800), CD226 (Novus Biologicals NBP1-85001, 1:50), and CD155 (Bioss Inc. bs-2525R, 1:750), and processed with the appropriate biotinylated secondary antibodies (Vector Laboratories, Burlingame CA) and avidin-biotin staining kit with diaminobenzidine as chromogen.

Techniques:

Journal: bioRxiv

Article Title: Differential Expression of the T cell Inhibitor TIGIT in Glioblastoma and Multiple Sclerosis

doi: 10.1101/591131

Figure Lengend Snippet: (A) Expression of TIGIT and CD226 measured by flow cytometry on CD4 and CD8 T cells from tumor infiltrates. (B) Percent of CD4 and CD8 T cells expressing TIGIT, CD226, and percent of CD226 + , CD4, and CD8 T cells co-expressing TIGIT (C). Histograms represent mean ± S.E.M. (D) Quantification of the frequency of TIGIT + , CD226 + , and TIGIT + among CD226 + for CD4 and CD8 circulating and tumor-infiltrating T cells. Frequencies were assessed by flow cytometry. Dots connected by a line represent the same patient. TIL = tumor infiltrating lymphocytes. Statistical significance was assessed by paired Student’s t test with a p-value threshold of 0.05; ns = non-significant.

Article Snippet: Serial sections were stained with primary antibodies against CD3 (Dako A 40452, 1:200), TIGIT (Santa Cruz sc-103349, 1:800), CD226 (Novus Biologicals NBP1-85001, 1:50), and CD155 (Bioss Inc. bs-2525R, 1:750), and processed with the appropriate biotinylated secondary antibodies (Vector Laboratories, Burlingame CA) and avidin-biotin staining kit with diaminobenzidine as chromogen.

Techniques: Expressing, Flow Cytometry

Journal: bioRxiv

Article Title: Differential Expression of the T cell Inhibitor TIGIT in Glioblastoma and Multiple Sclerosis

doi: 10.1101/591131

Figure Lengend Snippet: Expression of TIGIT and CD226 measured by flow cytometry on circulating CD4 (A) and CD8 (B) T cells from healthy donors (HD) and GBM patients. Quantification of the frequency of TIGIT + , CD226 + , and TIGIT + among CD226 + and PD-1 + for CD4 (C) and CD8 (D) circulating T cells. The values for GBM are the same as depicted in in the “Blood” group. Histograms represent mean ± S.E.M. Statistical significance was assessed by unpaired Student’s t test with a p-value threshold of 0.05; ns = non-significant.

Article Snippet: Serial sections were stained with primary antibodies against CD3 (Dako A 40452, 1:200), TIGIT (Santa Cruz sc-103349, 1:800), CD226 (Novus Biologicals NBP1-85001, 1:50), and CD155 (Bioss Inc. bs-2525R, 1:750), and processed with the appropriate biotinylated secondary antibodies (Vector Laboratories, Burlingame CA) and avidin-biotin staining kit with diaminobenzidine as chromogen.

Techniques: Expressing, Flow Cytometry

Journal: eLife

Article Title: CRIg, a tissue-resident macrophage specific immune checkpoint molecule, promotes immunological tolerance in NOD mice, via a dual role in effector and regulatory T cells

doi: 10.7554/eLife.29540

Figure Lengend Snippet: CTV-labeled CD4 + Foxp3(GFP) - Tconv cells were stimulated with anti-CD3/CD28, in the presence of plate-coated control Ig, or CRIg-Ig (5 ug/ml). Soluble Ig fusion proteins for CTLA-4, PD-1, VISTA, CD226 and TIGIT (from R and D) were added at indicated concentrations. T cell proliferation was analyzed by CTV dilutions at day 3. Shown are representative of three experiments.

Article Snippet: peptide, recombinant protein , CD226 Ig , R and D Systems , CAT#: 4436-DN-050 , .

Techniques: Labeling, Control

Journal: eLife

Article Title: CRIg, a tissue-resident macrophage specific immune checkpoint molecule, promotes immunological tolerance in NOD mice, via a dual role in effector and regulatory T cells

doi: 10.7554/eLife.29540

Figure Lengend Snippet:

Article Snippet: peptide, recombinant protein , CD226 Ig , R and D Systems , CAT#: 4436-DN-050 , .

Techniques: Generated, Purification, Control, Recombinant, Plasmid Preparation, Sequencing, Staining, TA Cloning, Reverse Transcription, SYBR Green Assay, Avidin-Biotin Assay, Software, DNA Methylation Assay

Journal: iScience

Article Title: Early TRAIL-engagement elicits potent multimodal targeting of melanoma by CD34 + progenitor cell-derived NK cells

doi: 10.1016/j.isci.2023.107078

Figure Lengend Snippet:

Article Snippet: anti-DNAM-1 PE (REA1040) , Miltenyi Biotec B.V. , Cat# 130-117-489; RRID: AB_2727959.

Techniques: Virus, Recombinant, Multiplexing, Activity Assay, Software