Journal: Molecules (Basel, Switzerland)

Article Title: Effect of Supplementation with the Combination of Se-Enriched Lentinula edodes Mycelium, Exogenous Enzymes, Acidifiers, Sodium Butyrate and Silicon Dioxide Nanoparticle Feed Additives on Selected Parameters in Calves.

doi: 10.3390/molecules27165163

Figure Lengend Snippet: Figure 2. Percentage of the lymphocyte (LYM) subpopulations containing surface antigens in the peripheral blood of experimental (E) and control (C) calves: (a) CD2+; (b) CD4+; (c) CD8+; (d) WC1+; (e) MHC class II, and (f) percentage of the monocyte (MON) subpopulation containing CD11b+

Article Snippet: The following mouse anti-bovine monoclonal antibodies (mAb) with fluorescein isothiocyanate (FITC) were used: CD2+, CD4+, CD8+, WC1+, MHC class II monomorphic, and CD11b+ (Bio-Rad, Hercules, USA).

Techniques: Control

Journal: Frontiers in pharmacology

Article Title: Exploration of the mechanism of Taohong Siwu Decoction for the treatment of ischemic stroke based on CCL2/CCR2 axis.

doi: 10.3389/fphar.2024.1428572

Figure Lengend Snippet: FIGURE 11 Expression of CD86 (M1) and CD206 (M2). (A): Expression of CD86 (M1); (B): Expression of CD206 (M2). #P < 0.05, ##P < 0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.

Article Snippet: A11042), were both purchased from MultiSciences, APC anti-Human CD206 (LW1218) was purchased from Elabscience, human TNF-α (July 2023), Human matrix metalloproteinase 9 (MMP-9) (July 2023), human IL-6 (July 2023), human IL-4 (July 2023), rat TNF-α (May 2023), ratMMP-9 (May 2023), rat IL-6 (May 2023), rat IL-4 (May 2023) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., claudin-5 (cat.no.

Techniques: Expressing

Journal: Cell reports

Article Title: A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome Instability

doi: 10.1016/j.celrep.2020.01.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse Anti-Human CD2 Monoclonal Antibody, Clone LT2 (1:10 for Flow) , Miltenyi Biotec , Cat# 130-091-115, RRID:AB_244321.

Techniques: Virus, Recombinant, Modification, Adhesive, Single Cell Gel Electrophoresis, TA Cloning, Sequencing, Generated, Software

Journal: Nucleic Acids Research

Article Title: NSrp70 is a lymphocyte-essential splicing factor that controls thymocyte development

doi: 10.1093/nar/gkab389

Figure Lengend Snippet: Deletion of NSrp70 results in defective survival signals following TCR activation in CD69 + DP thymocytes. ( A ) KEGG T cell receptor signaling pathway. The blue asterisks (*) represent down-regulated genes from the RNA-seq analysis (Figure ). ( B ) Expression of TCRβ, CD3ϵ, and CD3ζ on CD69 + DP thymocytes from Nsrp1 f/f (WT) and Nsrp1 f/f CD4Cre (KO) mice. ( C ) A schematic model of gene regulation by NSrp70. NSrp70 sequesters splicing factors in the nuclear speckles. Disintegration of splicing factors by NSrp70 deletion induces abnormal gene regulation during thymocyte development. As one of the results, reduced TCR expression may cause impaired T cell maturation. ( D ) Calcium flux in DP thymocytes. Cells from (A) were stimulated with PMA and ionomycin (P/I) or anti-CD3/CD28 antibodies, and then calcium fluxes were measured by flow cytometry. ( E ) Western blot of ZAP70, PKCθ, Erk1/2, and p38 in DP cell lysates stimulated on anti-CD3/28 for 0, 5, and 20 min. β-actin served as the loading control. M, molecular mass (KDa). ( F ) In vitro thymocyte development assay. CD69 – DP thymocytes were stimulated on anti-TCRβ/CD2 antibodies for 20 h (stimulation), or the cells were further incubated for 20 h in medium without stimulation (recovery). (G and H) Cells from (F) were stained for Ki-67 ( G ) or annexin V and 7ADD ( H ). Cells were analyzed by flow cytometry (F–H). The bar graphs indicate mean fluorescence intensities (MFI) (G). *, meaningful P -value; NC, non-coated; S, stimulation; R, recovery. The bar graphs indicate average ± standard deviation of apoptotic and dead thymocytes population (H). NS, non-significant P -value. All data shown are representative of three independent experiments.

Article Snippet: Anti-CD2 antibody (130-100-617) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).

Techniques: Activation Assay, RNA Sequencing, Expressing, Flow Cytometry, Western Blot, Control, In Vitro, Incubation, Staining, Fluorescence, Standard Deviation

Journal: Nucleic Acids Research

Article Title: The p400 complex promotes HIV-1 latency by suppressing viral transcription and altering the host cell state

doi: 10.1093/nar/gkaf764

Figure Lengend Snippet: EP400 depletion results in increased expression of activation-associated proteins in T cells. ( A ) Expression of CD2, CD69, and HIV in J-Lat 10.6 cells transduced with shRNAmirs targeting CD8B, CD19, or EP400 (two independent shRNAmirs) as measured by flow cytometry, three independent experiments. Upper panels: Representative histograms showing expression of the indicated proteins. Blue traces = expression of CD69 in cells transduced with control shRNAmirs. Red traces = expression of CD69 in cells transduced with EP400 shRNAmirs. Lower panels: Percentage of cells with high levels of expression of indicated proteins. * P <.05, ** P <.01, *** P <.001, **** P <.0001, NS = not significant. One-way ANOVA with multiple comparisons. ( B ) Representative flow cytometry plots showing expression of CD2, CD69, and HIV in in J-Lat 10.6 cells transduced with shRNAmirs targeting CD8B or EP400. ( C ) Representative western blot analysis of EP400, CD2, LEF-1, and GAPDH protein levels in J-Lat 10.6 cells from panel (A). ( D ) As for panel (C) but in uninfected Jurkat cells. ( E ) As for panel (C) but in primary CD4 + cells.

Article Snippet: Membranes were probed with antibodies to EP400 (BETHYL, #A300-541A), DMAP1 (Thermo Fisher, #10411-1-AP), HDAC1 (Thermo Fisher, #PA1-860), BRD4 (Proteintech, #ABE1391) LEF-1 (Cell Signaling, #76010S), CD2 (Cell Signaling, #53460S), or GAPDH (Santa Cruz, #sc-47724) followed by horseradish peroxidase-conjugated antirabbit or antimouse IgG.

Techniques: Expressing, Activation Assay, Transduction, Flow Cytometry, Control, Western Blot