Journal: International Journal of Molecular Sciences

Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury

doi: 10.3390/ijms22073551

Figure Lengend Snippet: Antibodies used for analysis of EV surface markers using vesicle flow cytometry.

Article Snippet: CD18 , M18/2 , APC , 130-104-019 , Miltenyi Biotec.

Techniques: Cytometry

Journal: Integrative Cancer Therapies

Article Title: Ze-qi Decoction Inhibits Neutrophil Extracellular Trap Formation to Suppress the Progression and Metastasis of Non-Small Cell Lung Cancer

doi: 10.1177/15347354261420750

Figure Lengend Snippet: ZQD inhibited pro-oncogenic neutrophil activation and NETs formation in NSCLC. (A) Western blotting showing that high-dose ZQD significantly downregulated the protein levels of HIF-1α, CD18, and ICAM-1, which are related to neutrophil activation, adhesion, and recruitment. (B) Immunofluorescence micrographs (200×) of the DAPI (blue fluorescence), Ly6G (green fluorescence), CitH3 (red fluorescence), and merge in model, cisplatin, ZQD-L, and ZQD-H groups. (C) Immunohistochemistry micrograph (200×) of myeloperoxidase (MPO) in the model, cisplatin, ZQD-L, and ZQD-H groups. (D) Enzyme-linked immunosorbent analysis (ELISA) of MPO showing consistent results with immunohistochemistry. (E) Western blotting showing that ZQD downregulated the expression of MPO, CitH3, and neutrophil elastase proteins. ZQD inhibited the expression of these proteins. Data are presented as mean ± SEM. Abbreviations: ns, no significance; SEM, standard error of the mean. *Cisplatin/ZQD-L/ZQD-H versus Model. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: CD18 (Cat# 10554-1-AP, RRID: AB_2877736) and neutrophil elastase (NE, Cat# 27642-1-AP, RRID: AB_2918126) antibodies were obtained from Proteintech (Wuhan, China).

Techniques: Activation Assay, Western Blot, Immunofluorescence, Fluorescence, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: For DKK1-, SPARC-, TIMP2-, COL1, integrin β2, or integrin α3 PLA, purified rabbit monoclonal anti-DKK1 (1:200, Cell Signaling Technology, Cat# 48367S), rabbit polyclonal anti-SPARC (1:200, Cell Signaling Technology, Cat# 5420S), mouse monoclonal anti-TIMP2 (1:200, R&D Systems, Cat# MAB971), mouse monoclonal anti-COL1 (1:200, Cell Signaling Technology, Cat# 66948S), goat polyclonal anti-integrin β1 (1:200, R&D Systems, Cat# AF1778), goat polyclonal anti-integrin β2 (1:200, R&D Systems, Cat# AF1730), and mouse monoclonal anti-integrin α3 (1:200, R&D Systems, Cat# MAB1345) were used.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Fundamental Research

Article Title: A dynamic peripheral immune landscape during human pregnancy

doi: 10.1016/j.fmre.2022.06.011

Figure Lengend Snippet: Aberrant cell-cell communication supported the decreased adaptive immune response after pregnancy. (a) Dot plot showing the representative GO biological process and pathways enriched in downregulated DEGs in DC and MC subsets. (b) Venn plot showing the integrated comparative analysis of downregulated DEGs among DC and MC subsets. (c) Circle plot showing the inferred MHC-II signaling networks. (d) Violin plot showing the expression of genes related to MHC-II signaling among immune populations between HC (blue) and Preg (red) groups. (e) Circle plot showing the inferred CD40 signaling networks. (f) Circle plot showing the inferred ICOS signaling networks in Preg group but absent in HC group. (g) Violin plot showing the expression of genes related to ICOS signaling among immune populations between HC (blue) and Preg (red) groups. (h) Circle plot showing the inferred MIF signaling networks. (i) Violin plot showing the expression of genes related to MIF signaling among immune populations between HC (blue) and Preg (red) groups. (j) The flow cytometry histogram showing the expression of CD74 in CD4 + TCs. Box plots showing the ratio of CD74-expressing CD4 + TCs between HC and Preg groups. (k) Violin plot showing the expression of genes related to ICAM signaling among immune populations between HC (blue) and Preg (red) groups. (l) The flow cytometry histogram showing the expression of CD18 in CD4 + TCs (left). Box plots showing the Mean Fluorescence Intensity (MFI) of CD18 in CD4 + TCs between HC and Preg groups (right). Significance in j, l was calculated using an unpaired, two-tailed Student's t-test; * P < 0.05.

Article Snippet: For the surface staining, PBMCs were simultaneously stained with anti-human fluorochrome-labeled antibodies specific for CD45 (BV785, #304048), CD3 (BV421, #300434), CD14 (PerCP/Cy5.5, #301824), CD4 (APC, #317416 or PE, #300508), CD8 (PerCP/Cy5.5, #301031), CD45RA (BV785, #304140), CD45RO (BV605, #304238), CD1C (PerCP/Cy5.5, #331514), CD19 (BV605, #302244), CD20 (APC, #302310), CD38 (BV785, #303530), HLA-DQ (PE, #318105), BCMA (PE-Cy7, #357508), CD74 (APC, #326811), CD69 (BV650, #310934), CD83 (PE, #305307) (BioLegend, San Diego, USA) and CD18 (APC, #E-AB-F1057E) (Elabscience).

Techniques: Expressing, Flow Cytometry, Fluorescence, Two Tailed Test

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Primer sequences and PCR settings

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Hybridization

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Expression of CD11a and CD18 in melanoma A375, 1205LU and SLM8 cell lines with conditioned medium. Cell-surface expression of CD11a and CD18 on indicated melanoma cell lines treated for 24 hrs with HUVEC conditioned medium was analyzed by flow cytometry. Isotypic controls are represented as empty histograms and specific antibody-labelling is displayed as shaded histograms. Histograms obtained with cells incubated with FCS-complete medium and labeled with specific antibodies, which overlap with the isotypic control are not shown. Data from obtained with 3 independent experiments.

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Expressing, Flow Cytometry, Incubation, Labeling, Control

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Effect of CD11a and CD18-blocking antibodies on the transendothelial migration of A375, 1205LU and SLM8 cell lines. The experiments were performed as detailed in Figure , except that 2μg/ml of CD11a or CD18-blocking antibodies were introduced in the upper chamber of the Transwells when indicated. Histograms represent 3 independent experiments. In each experiment each condition was analyzed in duplicate.

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Blocking Assay, Migration

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Effect of CD11 and CD18-blocking antibodies on the formation of clumps. A Semi-quantitative PCRs were performed to detect the expression of the ICAM-1 transcript. GAPDH is used as a DNA amount control. B A375, 1205LU and SLM8 cell lines were treated with 2 μg/ml of CD11a or CD18-blocking antibodies as indicated. Melanoma cells were labeled with DiO then fixed and labeled with DAPI prior to their observation under an epifluorescence microscope using a magnification of x10. Data were obtained from 3 independent experiments.

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Blocking Assay, Expressing, Control, Labeling, Microscopy