cd166 Search Results


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Developmental Studies Hybridoma Bank zn8
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Boster Bio antibodies against kdm1a
List of up-regulated proteins during Ang II infusion.
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Proteintech alcam
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
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Bio-Rad anti cd166 monoclonal antibodies mabs
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
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R&D Systems goat anti alcam af1172
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
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R&D Systems alcam
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
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Bioss anti‑cd166
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
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Miltenyi Biotec musc isolation
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
Musc Isolation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti goat antibody
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
Anti Goat Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec anti-human cd166-pe
a , b Expression <t>of</t> <t>CD44,</t> CD133, and <t>ALCAM</t> in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
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Image Search Results


List of up-regulated proteins during Ang II infusion.

Journal: Biomedicines

Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion

doi: 10.3390/biomedicines9121820

Figure Lengend Snippet: List of up-regulated proteins during Ang II infusion.

Article Snippet: The primary antibodies against KDM1A (BM4356), ALCAM (A01788-1), MBNL1 (A02309-1), and TSN (A02777) were purchased from Boster Biological Technology (Wuhan, Hubei, China).

Techniques: Ubiquitin Proteomics, Migration, Membrane

Immunohistochemical verification of DEPs. ( A ) Immunohistochemistry staining of up-regulated proteins, such as lysine-specific histone demethylase 1A (KDM1A), serine/threonine-protein kinase N1 (PKN1), C-terminal-binding protein 1 (CtBP1), transmembrane protein 41B (TMEM41B), myeloblastin (PRTN3), CD166 antigen (ALCAM), and GRB10-interacting GYF protein 2 (GIGYF2). Positive staining was indicated by brown coloration, and nuclei were stained with hematoxylin in blue. ( B ) Immunohistochemistry staining of down-regulated proteins, such as CCN family member 1 (CCN1), muscleblind-like protein 1 (MBNL1), H/ACA ribonucleoprotein complex subunit 2 (NHP2), retinol dehydrogenase 10 (RDH10), T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4), and Translin (TSN). Positive cells are indicated by brown coloration.

Journal: Biomedicines

Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion

doi: 10.3390/biomedicines9121820

Figure Lengend Snippet: Immunohistochemical verification of DEPs. ( A ) Immunohistochemistry staining of up-regulated proteins, such as lysine-specific histone demethylase 1A (KDM1A), serine/threonine-protein kinase N1 (PKN1), C-terminal-binding protein 1 (CtBP1), transmembrane protein 41B (TMEM41B), myeloblastin (PRTN3), CD166 antigen (ALCAM), and GRB10-interacting GYF protein 2 (GIGYF2). Positive staining was indicated by brown coloration, and nuclei were stained with hematoxylin in blue. ( B ) Immunohistochemistry staining of down-regulated proteins, such as CCN family member 1 (CCN1), muscleblind-like protein 1 (MBNL1), H/ACA ribonucleoprotein complex subunit 2 (NHP2), retinol dehydrogenase 10 (RDH10), T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4), and Translin (TSN). Positive cells are indicated by brown coloration.

Article Snippet: The primary antibodies against KDM1A (BM4356), ALCAM (A01788-1), MBNL1 (A02309-1), and TSN (A02777) were purchased from Boster Biological Technology (Wuhan, Hubei, China).

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Binding Assay

DEPs with high connectivity degree in PPI analysis between saline and Ang II infusion group.

Journal: Biomedicines

Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion

doi: 10.3390/biomedicines9121820

Figure Lengend Snippet: DEPs with high connectivity degree in PPI analysis between saline and Ang II infusion group.

Article Snippet: The primary antibodies against KDM1A (BM4356), ALCAM (A01788-1), MBNL1 (A02309-1), and TSN (A02777) were purchased from Boster Biological Technology (Wuhan, Hubei, China).

Techniques: Saline, Ubiquitin Proteomics

a , b Expression of CD44, CD133, and ALCAM in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test

Journal: Cell Death & Disease

Article Title: The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling

doi: 10.1038/s41419-019-1591-4

Figure Lengend Snippet: a , b Expression of CD44, CD133, and ALCAM in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test

Article Snippet: Antibodies against PAX6 (12323-1-AP), E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), vimentin (10366-1-AP), FSP-1 (16105-1-AP), GAPDH (66004-1-Ig), CD44 (15675-1-AP), CD133 (18470-1-AP), and ALCAM (21972-1-AP), as well as secondary antibodies were obtained from Proteintech Group, Inc. (Wuhan, China); antibodies against EGFR (201012-3F12), WNT5A (619919), ZEB2 (505705), Ki67 (200296), AKT (200323-5A11), phospho-AKT (Ser473) (310021), PI3K (220742), and phospho-PI3K p85alpha (Tyr607) (340790) were purchased from Zenbio (Chengdu, China).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Two Tailed Test

a Tumor volumes of subcutaneous Ctl or si-PAX6 or Vector or PAX6 overexpression xenografts during 21 days of treatment with PBS or CDDP. b , c Representative tumors isolated from nude mice and average tumor weights in the indicated groups; ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test. d IHC staining of PAX6, EMT-associated genes (E-cadherin, N-cadherin, vimentin, FSP-1), stem cell biomarkers (CD44, CD133, ALCAM), and the proliferation marker Ki67 in subcutaneous tumors of mice injected with Ctl vs. si-PAX6, Vector vs. PAX6 (scale bar, 50μm)

Journal: Cell Death & Disease

Article Title: The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling

doi: 10.1038/s41419-019-1591-4

Figure Lengend Snippet: a Tumor volumes of subcutaneous Ctl or si-PAX6 or Vector or PAX6 overexpression xenografts during 21 days of treatment with PBS or CDDP. b , c Representative tumors isolated from nude mice and average tumor weights in the indicated groups; ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test. d IHC staining of PAX6, EMT-associated genes (E-cadherin, N-cadherin, vimentin, FSP-1), stem cell biomarkers (CD44, CD133, ALCAM), and the proliferation marker Ki67 in subcutaneous tumors of mice injected with Ctl vs. si-PAX6, Vector vs. PAX6 (scale bar, 50μm)

Article Snippet: Antibodies against PAX6 (12323-1-AP), E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), vimentin (10366-1-AP), FSP-1 (16105-1-AP), GAPDH (66004-1-Ig), CD44 (15675-1-AP), CD133 (18470-1-AP), and ALCAM (21972-1-AP), as well as secondary antibodies were obtained from Proteintech Group, Inc. (Wuhan, China); antibodies against EGFR (201012-3F12), WNT5A (619919), ZEB2 (505705), Ki67 (200296), AKT (200323-5A11), phospho-AKT (Ser473) (310021), PI3K (220742), and phospho-PI3K p85alpha (Tyr607) (340790) were purchased from Zenbio (Chengdu, China).

Techniques: Plasmid Preparation, Over Expression, Isolation, Two Tailed Test, Immunohistochemistry, Marker, Injection