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Image Search Results
Journal: Biomedicines
Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion
doi: 10.3390/biomedicines9121820
Figure Lengend Snippet: List of up-regulated proteins during Ang II infusion.
Article Snippet: The primary
Techniques: Ubiquitin Proteomics, Migration, Membrane
Journal: Biomedicines
Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion
doi: 10.3390/biomedicines9121820
Figure Lengend Snippet: Immunohistochemical verification of DEPs. ( A ) Immunohistochemistry staining of up-regulated proteins, such as lysine-specific histone demethylase 1A (KDM1A), serine/threonine-protein kinase N1 (PKN1), C-terminal-binding protein 1 (CtBP1), transmembrane protein 41B (TMEM41B), myeloblastin (PRTN3), CD166 antigen (ALCAM), and GRB10-interacting GYF protein 2 (GIGYF2). Positive staining was indicated by brown coloration, and nuclei were stained with hematoxylin in blue. ( B ) Immunohistochemistry staining of down-regulated proteins, such as CCN family member 1 (CCN1), muscleblind-like protein 1 (MBNL1), H/ACA ribonucleoprotein complex subunit 2 (NHP2), retinol dehydrogenase 10 (RDH10), T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4), and Translin (TSN). Positive cells are indicated by brown coloration.
Article Snippet: The primary
Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Binding Assay
Journal: Biomedicines
Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion
doi: 10.3390/biomedicines9121820
Figure Lengend Snippet: DEPs with high connectivity degree in PPI analysis between saline and Ang II infusion group.
Article Snippet: The primary
Techniques: Saline, Ubiquitin Proteomics
Journal: Cell Death & Disease
Article Title: The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling
doi: 10.1038/s41419-019-1591-4
Figure Lengend Snippet: a , b Expression of CD44, CD133, and ALCAM in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG , SOX2 , and OCT4 , by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e , f : * P < 0.05, ** P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test
Article Snippet: Antibodies against PAX6 (12323-1-AP), E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), vimentin (10366-1-AP), FSP-1 (16105-1-AP), GAPDH (66004-1-Ig), CD44 (15675-1-AP), CD133 (18470-1-AP), and
Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Two Tailed Test
Journal: Cell Death & Disease
Article Title: The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling
doi: 10.1038/s41419-019-1591-4
Figure Lengend Snippet: a Tumor volumes of subcutaneous Ctl or si-PAX6 or Vector or PAX6 overexpression xenografts during 21 days of treatment with PBS or CDDP. b , c Representative tumors isolated from nude mice and average tumor weights in the indicated groups; ** P < 0.01, *** P < 0.001; two-tailed Student’s t -test. d IHC staining of PAX6, EMT-associated genes (E-cadherin, N-cadherin, vimentin, FSP-1), stem cell biomarkers (CD44, CD133, ALCAM), and the proliferation marker Ki67 in subcutaneous tumors of mice injected with Ctl vs. si-PAX6, Vector vs. PAX6 (scale bar, 50μm)
Article Snippet: Antibodies against PAX6 (12323-1-AP), E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), vimentin (10366-1-AP), FSP-1 (16105-1-AP), GAPDH (66004-1-Ig), CD44 (15675-1-AP), CD133 (18470-1-AP), and
Techniques: Plasmid Preparation, Over Expression, Isolation, Two Tailed Test, Immunohistochemistry, Marker, Injection