Journal: Sports Medicine - Open

Article Title: Acute Changes in Inflammatory Biomarker Levels in Recreational Runners Participating in a Marathon or Half-Marathon

doi: 10.1186/s40798-016-0045-0

Figure Lengend Snippet: Individual changes in mediators of inflammation in recreational athletes after running either a marathon or half-marathon race. * p < 0.05, ** p < 0.01, and *** p < 0.001 for comparisons between pre- and post-race values of all runners at given time points. § p < 0.05 and §§ p < 0.01 for comparisons of changes in values between full- and half-marathon runners. The highest levels of suPAR, CRP, IL-6, TNF-α, and IL-10 were noted from a marathon runner who also suffered from the most severe symptoms of post-race fatigue (case indicated by arrows ). CD163 macrophage receptor for haptoglobin-hemoglobin complexes, CRP C-reactive protein, IL interleukin, suPAR soluble urokinase-type plasminogen activator receptor, TGF-β transforming growth factor-β, TNF-α tumor necrosis factor-α, WBC white blood cells (leukocytes)

Article Snippet: The measurements of CD163 were carried out using Quantikine human CD163 ELISA assay (R&D Systems, Abingdon Science Park, UK).

Techniques:

Journal: Sports Medicine - Open

Article Title: Acute Changes in Inflammatory Biomarker Levels in Recreational Runners Participating in a Marathon or Half-Marathon

doi: 10.1186/s40798-016-0045-0

Figure Lengend Snippet: Correlations ( r ) between changes in biomarker levels, as calculated from percent change (pre to 3 h post) for each outcome variable

Article Snippet: The measurements of CD163 were carried out using Quantikine human CD163 ELISA assay (R&D Systems, Abingdon Science Park, UK).

Techniques: Biomarker Discovery

Journal: Cytokine

Article Title: Identification of promising plasma immune biomarkers to differentiate active pulmonary tuberculosis.

doi: 10.1016/j.cyto.2016.08.030

Figure Lengend Snippet: Fig. 3. Quantification of plasma levels of sCD163 and sCD14. The levels of soluble CD163 (a) and sCD14 (b) in plasma from pulmonary tuberculosis patients (APTB), latent infected subjects (LTBI) and non-infected controls (CTRL) were measured by ELISA. Each point is representative of an individual. The results, expressed in ng/mL, were analyzed by the non-parametric Mann Whitney test, p < 0.01667 (corrected by multiple comparitions).

Article Snippet: The concentration of soluble monocytes/macrophages plasma biomarkers CD14 and CD163 were measured using a DuoSet ELISA kit (R&D Systems, Minneapolis, Minn), according to the manufacturer’s instructions.

Techniques: Clinical Proteomics, Infection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Experimental and Therapeutic Medicine

Article Title: Role of SARS‑CoV‑2 nucleocapsid protein in affecting immune cells and insights on its molecular mechanisms

doi: 10.3892/etm.2023.12203

Figure Lengend Snippet: Quantification of TNF-α, IL-10, IL-6 and CD163. The concentrations of (A) TNF-α, (B) IL-10, (C) IL-6 and (D) CD163 were determined in the supernatant of cultured Raw264.7 cells treated with the severe acute respiratory syndrome coronavirus 2 N protein. Data are presented as mean ± SD (n=3). * P<0.05 and ** P<0.01 vs. blank control.

Article Snippet: PageRuler TM Prestained protein ladder (cat. no. 26616) was purchased from Thermo Fisher Scientific, Inc. Trypsin-EDTA (0.25%) used to digest Raw264.7 cells for cell passage was purchased from Gibco; Thermo Fisher Scientific Inc. TNF-α Elisa kit (Cat. no. EK0527), IL-6 Elisa Kit (Cat. no. EK0411), IL-10 Elisa Kit (Cat. no. EK0417) and CD163 ELISA kit (Cat. no. EK1146) were purchased from Boster Biological Technology co., Ltd.).

Techniques: Cell Culture, Control