Journal: Parasite immunology

Article Title: SEA Alleviates Hepatic Ischaemia-Reperfusion Injury by Promoting M2 Macrophage Polarisation.

doi: 10.1111/pim.13061

Figure Lengend Snippet: FIGURE 4 | SEA pretreatment promoted the polarisation of M2 macrophages. Mice were subjected to 60 min of hepatic ischaemia, followed by 6 h of reperfusion. (A) Representatives of immunofluorescence staining of CD68 (n = 5 per group, five biological replicates from five individual animals; original magnification 100× and 200×, scale bar: 100 μm). (B) Representatives of immunofluorescence staining of CD163 (n = 5 per group, five biological replicates from five individual animals; original magnification 100× and 200×, scale bar: 100 μm). (C, D) The expression of M2 macrophage marker (CD206 and Arg1) in the liver was detected via qRT-PCR (n = 5 per group, five biological replicates from five individual animals). These results were obtained from at least three independent experiments. Values are presented as mean ± SEM. **p < 0.01, *p < 0.05, ns > 0.05.

Article Snippet: Then the sections were incubated with primary antibodies dissolved in 5% donkey serum solution containing 0.3% Triton at 4°C overnight (anti- MPO, Cat# ab208670, Abcam, 1:100; anti- CD68, Cat# ab53444, Abcam, 1:100; anti- CD163, Cat# 16646- 1- AP, Proteintech, 1:100).

Techniques: Immunofluorescence, Staining, Expressing, Marker, Quantitative RT-PCR

Journal: Acta biomaterialia

Article Title: A subtype specific probe for targeted magnetic resonance imaging of M2 tumor-associated macrophages in brain tumors.

doi: 10.1016/j.actbio.2025.01.003

Figure Lengend Snippet: Fig. 6. Immunohistochemical and FACS validations of M2pep-uIONP targeting M2 TAMs. Confocal microscopic images of tumor tissues collected from mice at 24 h after i.v. injection of probes show (A) Cy7-M2pep-uIONP were highly co-localizing with CD68- and CD163-double positive M2 TAM, revealing the targeting specificity. In comparison, the non-targeted control probe Cy7-scM2pep-uIONP and Cy7-Ferumoxytol not only exhibited much less tumoral accumulation, but also were not co-localized with M2 TAM. Scale bars: 37 µm. (B) Flow cytometric zebra plots and (C) the corresponding quantification of CD68- and CD163-double positive M2 TAM targeted by FITC-M2pep-uIONP, FITC-scM2pep-uIONP, and FITC-Ferumoxytol. The gating strategy involves sorting CD163+ M2 TAM population in live cells dissociated from mouse cerebrums by gating on CD68+ pan-macrophage populations, followed by further gating to assess the FITC-labeled nanoparticles.

Article Snippet: Rabbit polyclonal anti-CD163 antibody was purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Immunohistochemical staining, Injection, Comparison, Control, Labeling

Journal: medRxiv

Article Title: M2 monocyte polarization in dialyzed patients is associated with increased levels of M-CSF and myeloperoxidase-associated oxidative stress: preliminary results

doi: 10.1101/2020.05.07.20094011

Figure Lengend Snippet: M1 and M2 monocytes in the blood of HD patients (n = 27) and healthy subjects (n = 23) were analyzed by flow cytometry. (A) Quantification of classical, intermediate and non-classical monocytes. Quantification of M1 monocytes assessed by the positive expression of CD86 and CCR2 (B) and of M2 monocytes assessed by the positive expression of CD206, CXCR3 and CD163 (C). Quantification of M1 (D) and M2 (E) monocytes in classical, intermediate and non-classical monocyte subsets. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: 100 μL of total blood from patients were incubated for 15 minutes at RT with PE mouse anti-human CD14 and V500 mouse anti-human CD16 antibodies (Becton Dickinson, Franklin Lakes, NJ, USA) as well as with mouse anti-human CD86-FITC and anti-human CCR2-APC monoclonal antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) for determining the M1 polarization or with mouse anti-human CD206-FITC, anti-human CXCR3-APC and anti-human CD163-VioBlue monoclonal antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) for the M2 polarization.

Techniques: Flow Cytometry, Expressing

Journal: BMC Veterinary Research

Article Title: Accumulation profiles of PrP Sc in hemal nodes of naturally and experimentally scrapie-infected sheep

doi: 10.1186/1746-6148-9-82

Figure Lengend Snippet: mAbs used to identify leukocyte subsets in hemal and retropharyngeal lymph nodes during immunohistochemistry study

Article Snippet: Mac , CD163 , MCA1853 , mouse IgG1 , 33.3 , AbD Serotec.

Techniques: Immunohistochemistry, Marker, Clone Assay