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Image Search Results
Journal: World Journal of Gastrointestinal Oncology
Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition
doi: 10.4251/wjgo.v17.i3.97673
Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition of colon cancer via the NLRP3 signaling pathway in mice. A and B: The positive expression and density of NLRP3 and E-cadherin were quantified using immunohistochemistry staining, with magnifications of 200 × (scale bar 100 μm) and 400 × (scale bar 50 μm); C and D: Flow cytometry was used to evaluate CD3 - NK1.1 + expression in colon cancer mice. n = 3 in each group. a P < 0.05; b P < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; EMT: Epithelial-mesenchymal transition; AOD: Average optical density.
Article Snippet: PBMCs were extracted from peripheral blood, and then were incubated with allophycocyanin Hamster anti-mouse CD3e (553066; BD Biosciences, United States),
Techniques: shRNA, Expressing, Immunohistochemistry, Staining, Flow Cytometry, Negative Control
Journal: World Journal of Gastrointestinal Oncology
Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition
doi: 10.4251/wjgo.v17.i3.97673
Figure Lengend Snippet: Short hairpin RNA targeting transmembrane protein 176B increased inhibition rate of interleukin-2-exposed natural killer cells on CT26 cells. A: Flow cytometry was used to evaluate CD3 - NK1.1 + cell number and CD107 expression post interleukin-2 (IL-2) stimulation in natural killer (NK) cells. n = 3 in each group; B: Quantitative reverse transcription PCR was applied to assess the relative expression of TMEM176B and NLRP3 in CT26 cells. n = 3 in each group; C: MTT assay was used to determine the inhibition rate of IL-2-exposed NK cells on CT26 cells with TMEM176B or NLRP3 knockdown over 24, 48, and 72 hours. n = 3 in each group. a P < 0.05; b P < 0.01. NK: Natural killer; sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; IL-2: Interleukin-2.
Article Snippet: PBMCs were extracted from peripheral blood, and then were incubated with allophycocyanin Hamster anti-mouse CD3e (553066; BD Biosciences, United States),
Techniques: shRNA, Inhibition, Flow Cytometry, Expressing, Reverse Transcription, MTT Assay, Knockdown, Negative Control
Journal: Scientific Reports
Article Title: The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells
doi: 10.1038/s41598-020-72181-6
Figure Lengend Snippet: Percentages of CD45 + immune cells in control and noise-exposed cochleae.
Article Snippet: The whole-mount preparations and sections were stained using the following antibodies at 1:50 to 1:100 dilution: goat anti-mouse CD45 (AF114, R&D Systems; a kind gift from Dr. Tejbeer Kaur), rat anti-mouse CD11b (MCA711, Bio-Rad), rat anti-mouse F4/80 (CI-A3-1: NB600-404SS, Novus Biologicals), rabbit monoclonal CD3e (SP7:SAB5500058, Sigma-Aldrich), rat anti-mouse CD45R/B220 (RA3- 6B2; 553083, BD Pharmingen), mouse monoclonal neutrophil elastase (NP57; sc-53388, Santa Cruz Biotechnology, Inc.),
Techniques: Control
Journal: Scientific Reports
Article Title: The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells
doi: 10.1038/s41598-020-72181-6
Figure Lengend Snippet: Immune cell phenotypic markers.
Article Snippet: The whole-mount preparations and sections were stained using the following antibodies at 1:50 to 1:100 dilution: goat anti-mouse CD45 (AF114, R&D Systems; a kind gift from Dr. Tejbeer Kaur), rat anti-mouse CD11b (MCA711, Bio-Rad), rat anti-mouse F4/80 (CI-A3-1: NB600-404SS, Novus Biologicals), rabbit monoclonal CD3e (SP7:SAB5500058, Sigma-Aldrich), rat anti-mouse CD45R/B220 (RA3- 6B2; 553083, BD Pharmingen), mouse monoclonal neutrophil elastase (NP57; sc-53388, Santa Cruz Biotechnology, Inc.),
Techniques: