Journal: International Journal of Molecular Sciences

Article Title: Cell State Transitions and Phenotypic Heterogeneity in Luminal Breast Cancer Implicating MicroRNAs as Potential Regulators

doi: 10.3390/ijms24043497

Figure Lengend Snippet: Expression profiling of stemness-associated markers in breast cancer. ( A ) Breast cancer cell lines were screened for the expression of the cell surface marker CD147/Basigin or EMMPRIN, CD151/tetraspanin, and drug transporter proteins CD243/ABCB1 and CD338/ABCG2. More than 5% of MCF7, BT474, and SKBR3 cells expressed drug transporter proteins (* comparison between unstained control and stained cells for each cell line, p ≤ 0.05; calculated by Student’s t -test). ( B ) Immunofluorescence staining for CD147 and CD151 (scale bar measures 75 µm). ( C ) Breast cancer cell lines MCF7, BT474, and SKBR3 showed positive expression for aldehyde dehydrogenase activity (ALDH), indicating the enrichment of cells with higher self-renewal and drug resistance potential.

Article Snippet: The panel of antibodies used for phenotypic characterization were CD147-FITC (Clone REA282), CD151-PE (Clone REA265), CD243/ABCB1-Viobright FITC (Clone REA495), and CD338/ABCG2-PerCP_Vio700 (Clone 5D3), purchased from Miltenyi Biotec, Gaithersburg, MD, USA).

Techniques: Expressing, Marker, Comparison, Control, Staining, Immunofluorescence, Activity Assay

Journal: OncoImmunology

Article Title: An epitope-specific novel anti-EMMPRIN polyclonal antibody inhibits tumor progression

doi: 10.1080/2162402x.2015.1078056

Figure Lengend Snippet: Figure 1: EMMPRIN structure and peptide design, and specificity of 161-Ab: (A) Partial

Article Snippet: One strip was probed with the 1:1,000 diluted commercial rat anti-mouse EMMPRIN (MAB772, R&D systems) and then with the 1:5,000 diluted HRP-conjugated goat anti-rat IgG (112-035- D ow nl oa de d by [ U ni ve rs ity o f R eg in a] a t 0 6: 58 2 4 Fe br ua ry 2 01 6 062, Jackson) and served as positive control (P.C).

Techniques:

Journal: Advanced Healthcare Materials

Article Title: Droplet Microarray Based Screening Identifies Proteins for Maintaining Pluripotency of hiPSCs

doi: 10.1002/adhm.202200718

Figure Lengend Snippet: Proteins used in this study with their corresponding structures and Protein Data Bank (PDB) codes

Article Snippet: Proteins (Thy‐1, EphB4, EphA1, E‐cadherin, coxackie and adenovirus receptor (CAR), JAM1, EpCAM, basigin (BSG), and dystroglycan (DAG1)) and rabbit anti‐CD147 antibody were purchased from Sino Biological Europe GmbH (Eschborn, Germany).

Techniques: Marker, Membrane, Protein Binding, Activation Assay, Migration, Stem Cell Culture

Journal: Cell Reports Medicine

Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases

doi: 10.1016/j.xcrm.2024.101755

Figure Lengend Snippet:

Article Snippet: APC-conjugated anti-human TRA-1-85 (CD147) , Miltenyi Biotec , Cat#130-128-900; RRID:AB_2921968.

Techniques: Virus, Recombinant, Staining, Labeling, Electron Microscopy, Bradford Assay, RNA Sequencing, Gene Expression, Knock-Out, CRISPR, Luciferase, Software, Fluorescence

Journal: British journal of cancer

Article Title: Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer.

doi: 10.1038/s41416-022-01729-2

Figure Lengend Snippet: Fig. 1 Representative images of IKKα immunohistochemical staining and controls. Example images of weak (a) and strong (b) cytoplasmic staining of IKKα. Representative images of true negative pancreatic tissue (c) and true positive kidney tissue (d) staining, respectively. Negative control colorectal tissue (e) with no antibody added during IHC staining process and colorectal tissue used as a positive control identified during optimisation (f). Western blot probed for IKKα and IKKβ using IKKα and IKKβ silenced lysates, MCF-7 cell pellets (IKKα silenced/IKKβ silenced) stained for IKKα as shown previously by Bennett et al. [3] (g). The optimal threshold for cytoplasmic IKKα histoscore was determined using R (h).

Article Snippet: Tissue microarrays were incubated overnight at 4 °C with anti-IKKα rabbit polyclonal antibody (GWB-66250 GenWay Biotech, California, USA) at a concentration of 1:1000.

Techniques: Immunohistochemical staining, Staining, Negative Control, Immunohistochemistry, Positive Control, Western Blot

Journal: British journal of cancer

Article Title: Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer.

doi: 10.1038/s41416-022-01729-2

Figure Lengend Snippet: Fig. 2 Representative images of punctate IKKα immunohistochemical staining and controls. Example images of low (a) and high (b) punctate staining of IKKα.

Article Snippet: Tissue microarrays were incubated overnight at 4 °C with anti-IKKα rabbit polyclonal antibody (GWB-66250 GenWay Biotech, California, USA) at a concentration of 1:1000.

Techniques: Immunohistochemical staining, Staining

Journal: British journal of cancer

Article Title: Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer.

doi: 10.1038/s41416-022-01729-2

Figure Lengend Snippet: Fig. 4 IKKα expression and survival. Expression of IKKα in the cytoplasm was not associated with CSS in the full cohort (a). High expression of cytoplasmic IKKα was associated with significantly worse cancer-specific survival in patients with right-sided tumours (b) but not in those with left-sided or rectal tumours (c, d). Punctate expression of IKKα was associated with CSS in the full cohort (e).

Article Snippet: Tissue microarrays were incubated overnight at 4 °C with anti-IKKα rabbit polyclonal antibody (GWB-66250 GenWay Biotech, California, USA) at a concentration of 1:1000.

Techniques: Expressing

Journal: British journal of cancer

Article Title: Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer.

doi: 10.1038/s41416-022-01729-2

Figure Lengend Snippet: Fig. 6 IKKα dual fluorescence with Rab5, Rab7 and Golgi 58. Co-localisation between IKKα (red) and Rab5 and Rab7 (green) was not observed in colorectal cancer tissue. DAPI (blue) represents nuclear counterstain. IKKα co-located with a Golgi marker (green) in colorectal cancer tissue.

Article Snippet: Tissue microarrays were incubated overnight at 4 °C with anti-IKKα rabbit polyclonal antibody (GWB-66250 GenWay Biotech, California, USA) at a concentration of 1:1000.

Techniques: Marker