Journal: International journal of oncology

Article Title: CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.

doi: 10.3892/ijo.2016.3812

Figure Lengend Snippet: Figure 1. The expression of CD133 in lung cancer and normal lung tissues. Original values are presented as log2 ratios. Spots showing the level of CD133 mRNA in NSCLC and normal tissues. (A) GSE10072. (B) GSE40275. (C) GSE63459; *p<0.05, **p<0.01 and ***p<0.001. (D) Correlation between CD133 and CXCR4 in GSE30219.

Article Snippet: Cells were incubated at 4 ̊C with 100 μl FcR blocking reagent and 100 μl CD133 Micro Beads (130-097-049, Miltenyi Biotec, Germany) for 30 min.

Techniques: Expressing

Journal: International journal of oncology

Article Title: CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.

doi: 10.3892/ijo.2016.3812

Figure Lengend Snippet: Figure 2. CD133 and CXCR4 are highly expressed in NSCLC patients with metastasis. (A) Representative images of immunohistochemistry staining (mag- nification, x400) of NSCLC patients. (a) CD133 low expression; (b) CD133 high expression; (c) CXCR4 low expression; (d) CXCR4 high expression. Positive staining ratio of CD133 (B)/CXCR4 (C) in metastatic and non-metastatic NSCLC patients. Positive staining score of CD133 (D)/CXCR4 (E) in metastatic and non-metastatic NSCLC patients. (F) Correlation results of CD133 and CXCR4. (G) Correlation analysis between CD133/CXCR4 co-expression and disease- free survival (red and black curves indicate high and low CD133/CXCR4 co-expression groups of patient death, respectively; *p<0.05).

Article Snippet: Cells were incubated at 4 ̊C with 100 μl FcR blocking reagent and 100 μl CD133 Micro Beads (130-097-049, Miltenyi Biotec, Germany) for 30 min.

Techniques: Immunohistochemistry, Staining, Expressing

Journal: International journal of oncology

Article Title: CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.

doi: 10.3892/ijo.2016.3812

Figure Lengend Snippet: Figure 3. CD133 enhances A549 cells proliferation. (A) Effectiveness of magnetic cell sorting was verified by immunofluorescence assay (magnification, x400). Red and blue indicate CD133 expression and cell nucleus, respectively. (B) Formation of colonies by A549 cell lines after 2-week incubation. (C and D) Cell proliferation capacity was determined in A549 cells, CD133+ A549 cells and CD133-A549 cells by CCK8 assays.

Article Snippet: Cells were incubated at 4 ̊C with 100 μl FcR blocking reagent and 100 μl CD133 Micro Beads (130-097-049, Miltenyi Biotec, Germany) for 30 min.

Techniques: FACS, Immunofluorescence, Expressing, Incubation

Journal: International journal of oncology

Article Title: CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.

doi: 10.3892/ijo.2016.3812

Figure Lengend Snippet: Figure 4. CXCR4 is upregulated by CD133. Silencing effectiveness of CD133 siRNA was verified by qPCR (A) and western blot analysis (B). (C) Expression of CXCR4 after treatment with CD133 siRNA (50 nM, 48 h) by qPCR analysis. (D) Western blot analysis for the expression of CXCR4 after treatment with CD133 siRNA (50 nM, 72 h). β-actin as a loading control. (E) The statistics of (D). All experiments were performed in triplicate. ***p<0.001.

Article Snippet: Cells were incubated at 4 ̊C with 100 μl FcR blocking reagent and 100 μl CD133 Micro Beads (130-097-049, Miltenyi Biotec, Germany) for 30 min.

Techniques: Western Blot, Expressing, Control

Journal: International journal of oncology

Article Title: CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.

doi: 10.3892/ijo.2016.3812

Figure Lengend Snippet: Figure 5. CD133+CXCR4+ promotes EMT process in NSCLC cells. (A) Transwell assay for the invasion of CD133-CXCR4+, CD133+CXCR4- and CD133+CXCR4+ cells. (B) Western blot assay for the expression of E-cadherin and Vimentin in CD133-CXCR4+, CD133+CXCR4- and CD133+CXCR4+ cells. (C) qPCR for the expression of E-cadherin, Vimentin, Snail, Slug and Twist in A549/CD133+, A549/CD133+ shRNA-NC, A549/CD133+ shRNA-CXCR4 and A549/CD133+ with amd3100. (D and E) RT-PCR for the expression of E-cadherin, Vimentin, Snail, Slug and Twist in A549/CD133+, A549/CD133+ shRNA-NC and A549/CD133+

Article Snippet: Cells were incubated at 4 ̊C with 100 μl FcR blocking reagent and 100 μl CD133 Micro Beads (130-097-049, Miltenyi Biotec, Germany) for 30 min.

Techniques: Transwell Assay, Western Blot, Expressing, shRNA, Reverse Transcription Polymerase Chain Reaction

Journal: International journal of oncology

Article Title: CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer.

doi: 10.3892/ijo.2016.3812

Figure Lengend Snippet: Figure 6. Vimentin is positively associated with CD133/CXCR4 co-expression. (A) Representative images of immunohistochemistry staining (magnification, x400) of NSCLC patients. (a) E-cadherin low expression; (b) E-cadherin high expression; (c) Vimentin low expression; (d) Vimentin high expression. Positive staining ratio of E-cadherin (B)/Vimentin (C) in metastatic and non-metastatic NSCLC patients. Correlation analysis for E-cadherin (D) or Vimentin (E) with CD133/CXCR4 co-expression in NSCLC patients.

Article Snippet: Cells were incubated at 4 ̊C with 100 μl FcR blocking reagent and 100 μl CD133 Micro Beads (130-097-049, Miltenyi Biotec, Germany) for 30 min.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Implanted adipose-derived stem cells attenuate small-for-size liver graft injury by secretion of VEGF in rats.

doi: 10.1111/j.1600-6143.2011.03870.x

Figure Lengend Snippet: Figure 1: Identification and purity of ADSCs. Flow cytometry revealed that the distribution of ADSCs that stained for CD14, CD31, CD34, CD45, CD68 and CD133 (shaded regions) did not differ from that of the isotype control (open regions). The majority of cells positively stained for CD29 and CD90 (shaded regions) compared with the isotype control cells (open regions) (A). Immunofluorescence analysis confirmed that ADSCs stained positively for CD29 and CD90, but not CD14, CD31, CD34, CD45, CD68 and CD133 (B). ADSCs resembled fibroblasts in culture and were successfully induced into adipocytes and osteocytes (C).

Article Snippet: Passage 3 cells were verified by immunostaining of surface markers for analysis by flow cytometry (Beckman Coulter, Brea, CA, USA) and immunofluorescence microscopy (Olympus, Tokyo, Japan) using fluorescent-conjugated antibodies against rat CD14, CD34, CD45, CD90 (Caltag Laboratories, San Francisco, CA, USA), CD29 (Biolegend, San Diego, CA, USA), CD31, CD68 (Abcam, Cambridge, MA, USA), CD133 (Novus Biologicals, Littleton, CO, USA) and respective isotypes.

Techniques: Flow Cytometry, Staining, Control

Journal: PloS one

Article Title: G protein-coupled receptor 87 (GPR87) promotes the growth and metastasis of CD133⁺ cancer stem-like cells in hepatocellular carcinoma.

doi: 10.1371/journal.pone.0061056

Figure Lengend Snippet: Figure 1. CD133+ HCC cells display high invasive and metastatic potential in vitro. (A) Isolation of CD133+ HCC cells sorted by MACS and detection of CD133 expression in SMMC-7721, HCC-LY5, SNU475, and PLC/PRF/5 cells by Western blot. (B) Growth curves of the sorted CD133+ and CD1332 SMMC-7721 and HCC-LY5 cells were obtained by the CCK-8 assay. (C) Transwell migration assay of CD133+ and CD1332 cells sorted from the SMMC-7721 and HCC-LY5 cell lines. (D) Transwell matrigel invasion assay of CD133+ and CD1332 cells sorted from the SMMC-7721 and HCC-LY5 cell lines. doi:10.1371/journal.pone.0061056.g001

Article Snippet: The CD133 cDNA clone (Myc-DDK-tagged ORF clone of Homo sapiens prominin 1 (PROM1), transcript variant 1 as transfectionready DNA NM_006017.1) with full length ORF sequence was purchased from Origene (OriGene Technologies, Inc. Rockville), which was cloned into the pWPXL lentiviral expression vector (Addgene, MA) by replacing the GFP fragment.

Techniques: In Vitro, Isolation, Expressing, Western Blot, CCK-8 Assay, Transwell Migration Assay, Invasion Assay

Journal: PloS one

Article Title: G protein-coupled receptor 87 (GPR87) promotes the growth and metastasis of CD133⁺ cancer stem-like cells in hepatocellular carcinoma.

doi: 10.1371/journal.pone.0061056

Figure Lengend Snippet: Figure 2. CD133+ HCC cells exhibit highly metastatic characteristics in vivo. (A) Representative in vivo bioluminescence imaging of tumor metastases in NOD/SCID mice after orthotopic transplantation with the isolated CD133+ or CD1332 SMMC-7721 cells (picture shown is representative of the group orthotopically transplanted with 10,000 cells) (n = 5 each group). (B) Representative examples of NOD/SCID mice orthotopically transplanted with CD133+ or CD1332 cells isolated from the SMMC-7721 HCC cell line. (C) Numbers of mice manifesting tumorigenicity and liver metastases of CD133+ SMMC-7721 cells in the orthotopic transplant assays are shown in the table. (D) HE staining of the harvested tumors confirmed a primary HCC phenotype. (E) Representative in vivo bioluminescence imaging of tumor metastasis in the NOD/SCID mice in a tumor-homing animal model transplanted with CD133+ or CD1332 cells isolated from the SMMC-7721 cell line (n = 9 each group). (F) Representative examples of the liver tumor formation and metastasis in 2-AAF/PHx animal model transplanted with CD133+ or CD1332 cells isolated from the SMMC-7721 HCC cell line. (G) HE staining of liver tissue sections, a tumor mass from CD133+ HCC cells in 2-AAF/PHx animal model was showed. (H) Numbers of mice manifesting liver metastases of CD133+ or CD1332 SMMC-7721 cells in the tumor-homing animal model are shown in the bar gragh. doi:10.1371/journal.pone.0061056.g002

Article Snippet: The CD133 cDNA clone (Myc-DDK-tagged ORF clone of Homo sapiens prominin 1 (PROM1), transcript variant 1 as transfectionready DNA NM_006017.1) with full length ORF sequence was purchased from Origene (OriGene Technologies, Inc. Rockville), which was cloned into the pWPXL lentiviral expression vector (Addgene, MA) by replacing the GFP fragment.

Techniques: In Vivo, Imaging, Transplantation Assay, Isolation, Staining, Animal Model

Journal: PloS one

Article Title: G protein-coupled receptor 87 (GPR87) promotes the growth and metastasis of CD133⁺ cancer stem-like cells in hepatocellular carcinoma.

doi: 10.1371/journal.pone.0061056

Figure Lengend Snippet: Figure 3. Detection of GPR87 expression in HCC cell lines and human primary cells by qRT-PCR and Western blot analysis. (A) Relative mRNA expression levels of GPR87 and CD133 were determined by quantitative polymerase chain reaction in the CD133+ and CD1332

Article Snippet: The CD133 cDNA clone (Myc-DDK-tagged ORF clone of Homo sapiens prominin 1 (PROM1), transcript variant 1 as transfectionready DNA NM_006017.1) with full length ORF sequence was purchased from Origene (OriGene Technologies, Inc. Rockville), which was cloned into the pWPXL lentiviral expression vector (Addgene, MA) by replacing the GFP fragment.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction

Journal: PloS one

Article Title: G protein-coupled receptor 87 (GPR87) promotes the growth and metastasis of CD133⁺ cancer stem-like cells in hepatocellular carcinoma.

doi: 10.1371/journal.pone.0061056

Figure Lengend Snippet: Figure 4. Overexpression of GPR87 up-regulates CD133 expression and enhances CSC-related properties. (A) Representative examples of proliferation assays examining the effect of GPR87 overexpression in SMMC-7721 and HCC-LY5 cells. (B) The gross features of the tumor-bearing NOD/SCID mice orthotopically transplanted with 26106 SMMC-7721-lenti-GPR87 and SMMC-7721-lenti- pWPXL cells after 6 weeks (n = 6 each group). (C) Transwell migration assay in SMMC-7721 and HCC-LY5 cells overexpressing GPR87. (D) Transwell matrigel invasion assay in SMMC-7721 and HCC- LY5 cells overexpressing GPR87. (E) Immunohistochemical staining of GPR87 and CD133 in HCC tissues with intrahepatic metastasis. (F) The table showed the correlation between CD133 and GPR87 expression in HCC tissues with intrahepatic metastasis. doi:10.1371/journal.pone.0061056.g004

Article Snippet: The CD133 cDNA clone (Myc-DDK-tagged ORF clone of Homo sapiens prominin 1 (PROM1), transcript variant 1 as transfectionready DNA NM_006017.1) with full length ORF sequence was purchased from Origene (OriGene Technologies, Inc. Rockville), which was cloned into the pWPXL lentiviral expression vector (Addgene, MA) by replacing the GFP fragment.

Techniques: Over Expression, Expressing, Transwell Migration Assay, Invasion Assay, Immunohistochemical staining, Staining

Journal: PloS one

Article Title: G protein-coupled receptor 87 (GPR87) promotes the growth and metastasis of CD133⁺ cancer stem-like cells in hepatocellular carcinoma.

doi: 10.1371/journal.pone.0061056

Figure Lengend Snippet: Figure 5. Silencing of GPR87 reduces the levels of CD133 expression. Flow cytometric analysis of the levels of CD133 expression in GPR87 siRNA-treated cells, including the PLC/PRF/5, Hep3B, SNU475 and Huh-7 cell lines. doi:10.1371/journal.pone.0061056.g005

Article Snippet: The CD133 cDNA clone (Myc-DDK-tagged ORF clone of Homo sapiens prominin 1 (PROM1), transcript variant 1 as transfectionready DNA NM_006017.1) with full length ORF sequence was purchased from Origene (OriGene Technologies, Inc. Rockville), which was cloned into the pWPXL lentiviral expression vector (Addgene, MA) by replacing the GFP fragment.

Techniques: Expressing

Journal: PloS one

Article Title: G protein-coupled receptor 87 (GPR87) promotes the growth and metastasis of CD133⁺ cancer stem-like cells in hepatocellular carcinoma.

doi: 10.1371/journal.pone.0061056

Figure Lengend Snippet: Figure 6. GPR87 mediates the expression of CD133 in HCC cell lines. (A) Relative mRNA expression of CD133 in SMMC-7721-lenti- CD133, HCC-LY5-lenti-CD133 and MHCC-97L-lenti-CD133 cells. (B) Relative mRNA expression of GPR87 in SMMC-7721-lenti-CD133, HCC- LY5-lenti-CD133 and MHCC-97L-lenti-CD133 cells. (C) Western blot of GPR87 and CD133 in SMMC-7721-lenti-CD133, HCC-LY5-lenti-CD133 and MHCC-97L-lenti-CD133 cells. doi:10.1371/journal.pone.0061056.g006

Article Snippet: The CD133 cDNA clone (Myc-DDK-tagged ORF clone of Homo sapiens prominin 1 (PROM1), transcript variant 1 as transfectionready DNA NM_006017.1) with full length ORF sequence was purchased from Origene (OriGene Technologies, Inc. Rockville), which was cloned into the pWPXL lentiviral expression vector (Addgene, MA) by replacing the GFP fragment.

Techniques: Expressing, Western Blot

Journal: OncoTargets and therapy

Article Title: NFATC2 is a novel therapeutic target for colorectal cancer stem cells

doi: 10.2147/OTT.S169129

Figure Lengend Snippet: NFATC2 is upregulated in CRC-SCs. ( A ) qRT-PCR analysis of NFATC2 in primary CRC spheres and re-adherent cells relative to adherent cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1, 2, 3, and 4). Spheres were obtained by suspension culture. ( B ) Western blot analysis of NFATC2 in primary CRC spheres and adherent and re-adherent cells. ( C ) qRT-PCR analysis of NFATC2 in sorted CD44 + (left) or CD133 + (right) primary CRC cells relative to negative cells. Primary CRC cells were isolated from the cancer tissues of colorectal cancer patients (No 1, 2, 3, and 4). CD44 + or CD133 + cells were obtained by flow cytometry. ( D ) The correlation between the transcription level of NFATC2 and CD44 (left) and CD133 (right) in pin primary CRC sphere-derived cells. The mRNA level of each gene was determined by qRT-PCR. Data were normalized to GAPDH as ∆CT and analyzed by Spearman’s correlation analysis. Data are represented as mean ± SD; * P <0.05, *** P <0.001; two-tailed Student’s t -test. Abbreviations: CRC, colorectal cancer; NFATC2, nuclear factor of activated T-cells, cytoplasmic 2; CRC-SCs, colorectal cancer stem cells; qRT-PCR, quantitative real-time polymerase chain reaction.

Article Snippet: CD133 , IB , Mouse monoclonal , , Miltenyi Biotec , 130-092-395 , 100.

Techniques: Quantitative RT-PCR, Isolation, Suspension, Western Blot, Flow Cytometry, Derivative Assay, Two Tailed Test, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: NFATC2 is a novel therapeutic target for colorectal cancer stem cells

doi: 10.2147/OTT.S169129

Figure Lengend Snippet: Overexpression of NFATC2 promotes the stemness of CRC cells. ( A , B ) Sphere formation assay of NFATC2-overexpressing and control primary CRC cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1 and 2). The 1st passaged spheres were obtained by suspension culture for 15 days and the number and average diameters of the spheres were counted ( A ). The number of 1st, 2nd, and 3rd passaged spheres isolated from the cancer tissues of CRC patients (No 1, 2, 3, and 4) was also counted ( B ). ( C ) qRT-PCR analysis of CD44 (left) and CD133 (right) in NFATC2-overexpressing and control primary CRC cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1 and 2). NFATC2-overexpressing and control cells were generated by lentivirus delivery system. ( D ) Western blot analysis of CD44 and CD133 in NFATC2-overexpressing and control primary CRC cells. Data are represented as mean ± SD; *** P <0.001; two-tailed Student’s t -test. Abbreviations: CRC, colorectal cancer; NFATC2, nuclear factor of activated T-cells, cytoplasmic 2; CRC-SCs, colorectal cancer stem cells; qRT-PCR, quantitative real-time polymerase chain reaction.

Article Snippet: CD133 , IB , Mouse monoclonal , , Miltenyi Biotec , 130-092-395 , 100.

Techniques: Over Expression, Tube Formation Assay, Control, Isolation, Suspension, Quantitative RT-PCR, Generated, Western Blot, Two Tailed Test, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: NFATC2 is a novel therapeutic target for colorectal cancer stem cells

doi: 10.2147/OTT.S169129

Figure Lengend Snippet: Knockdown of NFATC2 inhibits the stemness of CRC cells. ( A , B ) Sphere formation assay of NFATC2-knockdown and control primary CRC cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1 and 2). The 1st passaged spheres were obtained by suspension culture for 15 days and the number and average diameters of the spheres were counted ( A ). The number of 1st, 2nd, and 3rd passaged spheres was also counted ( B ). ( C ) qRT-PCR analysis of CD44 (top) and CD133 (bottom) in NFATC2-knockdown and control primary CRC cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1 and 2). NFATC2-knockdown and control cells were generated by lentivirus delivery system. ( D ) Western blot analysis of CD44 and CD133 in NFATC2-knockdown and control primary CRC cells. ( E ) Tumorigenesis of NFATC2-knockdown and control primary CRC cells. Data are represented as mean ± SD; *** P <0.001; two-tailed Student’s t -test. Abbreviations: CRC, colorectal cancer; NFATC2, nuclear factor of activated T-cells, cytoplasmic 2; CRC-SCs, colorectal cancer stem cells; qRT-PCR, quantitative real-time polymerase chain reaction.

Article Snippet: CD133 , IB , Mouse monoclonal , , Miltenyi Biotec , 130-092-395 , 100.

Techniques: Knockdown, Tube Formation Assay, Control, Isolation, Suspension, Quantitative RT-PCR, Generated, Western Blot, Two Tailed Test, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: NFATC2 is a novel therapeutic target for colorectal cancer stem cells

doi: 10.2147/OTT.S169129

Figure Lengend Snippet: YAP activity is necessary for NFATC2 for maintaining the stemness in CRC cells. ( A , B ) Sphere formation assay of YAP-knockdown NFATC2-overexpressing primary CRC cells and control cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1 and 2). The 1st passaged spheres were obtained by suspension culture for 15 days and the number of the spheres were counted ( A ). The number of 1st, 2nd, and 3rd passaged spheres was also counted ( B ). ( C ) qRT-PCR analysis of CD44 (top) and CD133 (bottom) in YAP-knockdown NFATC2-overexpressing primary CRC cells and control cells. Primary CRC cells were isolated from the cancer tissues of CRC patients (No 1 and 2). YAP-knockdown NFATC2-overexpressing primary CRC cells and control cells were generated by lentivirus delivery system. ( D ) Western blot analysis of CD44 and CD133 in YAP-knockdown NFATC2-overexpressing primary CRC cells and control cells. ( E ) Tumorigenesis of YAP-knockdown NFATC2-overexpressing primary CRC cells and control cells. Data are represented as mean ± SD; *** P <0.001; two-tailed Student’s t -test. Abbreviations: CRC, colorectal cancer; NFATC2, nuclear factor of activated T-cells, cytoplasmic 2; CRC-SCs, colorectal cancer stem cells; qRT-PCR, quantitative real-time polymerase chain reaction.

Article Snippet: CD133 , IB , Mouse monoclonal , , Miltenyi Biotec , 130-092-395 , 100.

Techniques: Activity Assay, Tube Formation Assay, Knockdown, Control, Isolation, Suspension, Quantitative RT-PCR, Generated, Western Blot, Two Tailed Test, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: NFATC2 is a novel therapeutic target for colorectal cancer stem cells

doi: 10.2147/OTT.S169129

Figure Lengend Snippet: Primer sequences used in this study

Article Snippet: CD133 , IB , Mouse monoclonal , , Miltenyi Biotec , 130-092-395 , 100.

Techniques: Reverse Transcription

Journal: OncoTargets and therapy

Article Title: NFATC2 is a novel therapeutic target for colorectal cancer stem cells

doi: 10.2147/OTT.S169129

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: CD133 , IB , Mouse monoclonal , , Miltenyi Biotec , 130-092-395 , 100.

Techniques:

Journal: Genes to Cells

Article Title: Functional Role of COP1 Gene in Hepatocellular Carcinoma Lipid Metabolism and Stemness

doi: 10.1111/gtc.70108

Figure Lengend Snippet: COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 CD133+ cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.

Article Snippet: The cells were treated with FcR Blocking Reagent and CD133 MicroBeads (130‐100‐857, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions and incubated for 15 min at 4°C in the dark.

Techniques: Knockdown, Migration, Control, Tube Formation Assay

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Effect of Shogaol on the Expression of Intestinal Stem Cell Markers in Experimentally Induced Colitis in BALB/c Mice

doi: 10.1155/2019/5134156

Figure Lengend Snippet: Cytoplasmic and membranous CD133 expression in epithelial cells of the colonic crypts. (a) Minimal, week expression (sum score 0) in mice of control negative group. (b) Focal, weak expression (sum score 1) in control positive group. (c) Focal, weak-moderate expression (sum score 4) deeply within the colonic crypt in group 3 (DSS exposure & 6-TG treatment). (d) Diffuse, weak expression (sum score 3) in group 4 (DSS exposure & 10 mg/kg b.w. Shogaol treatment). (e) and (f) Diffuse, moderate expression (sum score 6) extends into the transient amplifying and apical regions of the colonic crypts in group 5 (DSS exposure & 20 mg/kg b.w. Shogaol treatment) and group 6 (DSS exposure & 40 mg/kg b.w. Shogaol treatment), respectively.

Article Snippet: Following that, the slides were divided into 2 sets: the first set was incubated overnight at 4°C with rabbit anti-CD133 polyclonal Abs (1 : 100, San Francisco Biorbyt, USA), and the second set was incubated with rabbit anti-CD34 monoclonal Abs (1 : 100, Dako, Germany).

Techniques: Expressing, Control

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Effect of Shogaol on the Expression of Intestinal Stem Cell Markers in Experimentally Induced Colitis in BALB/c Mice

doi: 10.1155/2019/5134156

Figure Lengend Snippet: Immunofluorescence expression of the CD133 protein in the cytoplasm of the colonic cryptal epithelium. (a) and (b) Weak (+) expression in mice of the control negative and control positive group. (c) and (d) Weak-moderate (+ to ++) expression in the in deep portions of the colonic crypts in mice of group 3 (6-TG treatment) and group 4 (10 mg/kg b.w. Shogaol treatment). (e) and (f) Moderate (++) expression in mice of group 5 (20 mg/kg b.w. Shogaol treatment) and mice of group 6 (40 mg/kg b.w. Shogaol treatment).

Article Snippet: Following that, the slides were divided into 2 sets: the first set was incubated overnight at 4°C with rabbit anti-CD133 polyclonal Abs (1 : 100, San Francisco Biorbyt, USA), and the second set was incubated with rabbit anti-CD34 monoclonal Abs (1 : 100, Dako, Germany).

Techniques: Immunofluorescence, Expressing, Control

Journal: Cancer Management and Research

Article Title: Different Proteins Regulated Apoptosis, Proliferation and Metastasis of Lung Adenocarcinoma After Radiotherapy at Different Time

doi: 10.2147/CMAR.S219967

Figure Lengend Snippet: Differences in stem cells of XWLC-05 or A549 cells during radiation. ( A ) CD44 expression in A549 cells with time after 0, 4, 8 Gy irradiation. ( B ) CD44 expression in XWLC-05 with time after 0, 4, 8 Gy irradiation. ( C ) CD133 expression in A549 cells with time after 0, 4, 8 Gy irradiation. ( D ) CD133 expression in XWLC-05 with time after 0, 4, 8 Gy irradiation. ( E ) CD444+ and CD133+ cells in irradiated transplantation group A549 and XWLC-05 tumors. ( F ) CD444+CD133+ cells in irradiated transplantation group A549 and XWLC-05 tumors.

Article Snippet: For CD133 staining, 1 × 10 6 cells were stained with 20 μL CD133 mouse mAb (CST, USA).

Techniques: Expressing, Irradiation, Transplantation Assay