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Miltenyi Biotec
human cd117 microbead kit ![]() Human Cd117 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/cd117+microbead+kit/pm38168103-275-10-18?v=Miltenyi+Biotec Average 96 stars, based on 1 article reviews
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Miltenyi Biotec
cd117 microbeads, mouse ![]() Cd117 Microbeads, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/cd117+microbead+kit/custom%40130-091-224%4036152632?v=Miltenyi+Biotec Average 96 stars, based on 1 article reviews
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Image Search Results
Journal: Nature communications
Article Title: Synovial microenvironment-influenced mast cells promote the progression of rheumatoid arthritis.
doi: 10.1038/s41467-023-44304-w
Figure Lengend Snippet: Fig. 1 | Frequency of synovial mast cells was increased in RA patients and correlated with disease severity. a Synovial membranes from OA and RA patients were digested into single-cell suspension. Synovial mast cells were gated as CD45+CD117+FcεRI+ cells. Numbers indicated the percentages of mast cells in the gates. b Statistical analysis of mast cell percentage in synovial single-cell suspen- sions and CD45+ immune cells (n = 10 biologically independent samples for each group, P = 0.0328 for percentage in suspensions, P = 0.0196 for percentage in CD45+ cells). c Synovial infiltrating mast cells were defined by tryptase immuno- fluorescence staining. Red, tryptase; Blue, DAPI. Scale bar: 75 μm. d Statistical analysis of mast cell numbers per high power field (HPF) in synovial sections (n = 10 biologically independent samples in OA group, n = 11 biologically independent samples in RA group, P = 0.0001). e Ki-67 expression on synovial mast cells was
Article Snippet: CD117-positive cells were isolated from synovial single-cell suspensions using a
Techniques: Suspension, Staining, Expressing
Journal: Nature communications
Article Title: Synovial microenvironment-influenced mast cells promote the progression of rheumatoid arthritis.
doi: 10.1038/s41467-023-44304-w
Figure Lengend Snippet: Fig. 2 | RA synovial microenvironment-induced activation of mast cells. Syno- vial mast cells were enriched from digested synovial tissues of OA and RA patients by magnetic bead sorting for CD117+ cells and used for label-free mass spectro- metry. a Mast cell purity was confirmed by tryptase staining of enriched CD117+cells. Red, tryptase; Blue, DAPI. Scale bar: 25 μm. Data are representative of three independent experiments. b Heatmap of top 50 differentially expressed proteins between synovial mast cells from OA and RA patients by hierarchical clustering after z-score normalization. Upregulated and downregulated proteins in RA synovial mast cells were depicted in red and blue, respectively. c, d GO analysis and KEGG pathway analysis of differentially expressed proteins showed enriched terms including mitochondria metabolism, energy metabolism, and leukocyte
Article Snippet: CD117-positive cells were isolated from synovial single-cell suspensions using a
Techniques: Activation Assay, Staining