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Primary and secondary antibodies used in this study
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Fig. 6 | Co-culture assays of CAFs and B cells. a Schematic diagram showing the B cell adhesion assay (left panel). Bar plots showing the capability of B cell adhesion to CAFs (right panel; n = 3). B cells were cocultured with either <t>VCAM1-</t> CAFs, VCAM1+ CAFs, or VCAM1+ CAFs in combination with a VCAM1 neutralising antibody. The relative number (OD value) of B cells bound to CAFs were quantified using CCK8 assays. b Bar plot showing the relative expression level of CXCL13 in fibro- blasts (left panel; n = 3). Fibroblasts were transduced with an empty vector or CXCL13 plasmid. Bar plot illustrates the migration capabilities of B cells under various co-culture conditions with fibroblasts (right panel; n = 11). The B cells were co-cultured with fibroblasts overexpressing either an empty vector (Con_EV), CXCL13 alone, or CXCL13 in combination with a CXCL13-specific neutralising antibody. c Schematic diagram showing the B cell co-culture assay (left panel). Bar
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Fig. 6 | Co-culture assays of CAFs and B cells. a Schematic diagram showing the B cell adhesion assay (left panel). Bar plots showing the capability of B cell adhesion to CAFs (right panel; n = 3). B cells were cocultured with either <t>VCAM1-</t> CAFs, VCAM1+ CAFs, or VCAM1+ CAFs in combination with a VCAM1 neutralising antibody. The relative number (OD value) of B cells bound to CAFs were quantified using CCK8 assays. b Bar plot showing the relative expression level of CXCL13 in fibro- blasts (left panel; n = 3). Fibroblasts were transduced with an empty vector or CXCL13 plasmid. Bar plot illustrates the migration capabilities of B cells under various co-culture conditions with fibroblasts (right panel; n = 11). The B cells were co-cultured with fibroblasts overexpressing either an empty vector (Con_EV), CXCL13 alone, or CXCL13 in combination with a CXCL13-specific neutralising antibody. c Schematic diagram showing the B cell co-culture assay (left panel). Bar
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Image Search Results


Primary and secondary antibodies used in this study

Journal: Neural Regeneration Research

Article Title: ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling

doi: 10.4103/1673-5374.339007

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: Rat monoclonal anti- mouseVCAM1 , R and D Systems, MAB6434 , AB_2214050.

Techniques:

Fig. 6 | Co-culture assays of CAFs and B cells. a Schematic diagram showing the B cell adhesion assay (left panel). Bar plots showing the capability of B cell adhesion to CAFs (right panel; n = 3). B cells were cocultured with either VCAM1- CAFs, VCAM1+ CAFs, or VCAM1+ CAFs in combination with a VCAM1 neutralising antibody. The relative number (OD value) of B cells bound to CAFs were quantified using CCK8 assays. b Bar plot showing the relative expression level of CXCL13 in fibro- blasts (left panel; n = 3). Fibroblasts were transduced with an empty vector or CXCL13 plasmid. Bar plot illustrates the migration capabilities of B cells under various co-culture conditions with fibroblasts (right panel; n = 11). The B cells were co-cultured with fibroblasts overexpressing either an empty vector (Con_EV), CXCL13 alone, or CXCL13 in combination with a CXCL13-specific neutralising antibody. c Schematic diagram showing the B cell co-culture assay (left panel). Bar

Journal: Nature communications

Article Title: Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma.

doi: 10.1038/s41467-024-52153-4

Figure Lengend Snippet: Fig. 6 | Co-culture assays of CAFs and B cells. a Schematic diagram showing the B cell adhesion assay (left panel). Bar plots showing the capability of B cell adhesion to CAFs (right panel; n = 3). B cells were cocultured with either VCAM1- CAFs, VCAM1+ CAFs, or VCAM1+ CAFs in combination with a VCAM1 neutralising antibody. The relative number (OD value) of B cells bound to CAFs were quantified using CCK8 assays. b Bar plot showing the relative expression level of CXCL13 in fibro- blasts (left panel; n = 3). Fibroblasts were transduced with an empty vector or CXCL13 plasmid. Bar plot illustrates the migration capabilities of B cells under various co-culture conditions with fibroblasts (right panel; n = 11). The B cells were co-cultured with fibroblasts overexpressing either an empty vector (Con_EV), CXCL13 alone, or CXCL13 in combination with a CXCL13-specific neutralising antibody. c Schematic diagram showing the B cell co-culture assay (left panel). Bar

Article Snippet: Then, VCAM1+ or VCAM1- CAFs (2 × 104 per well) were seeded on a 96-well plate foroneday.On the secondday, CAFswere incubated with the presence or absence of neutralising antibody anti-VCAM1 Antibody (30μg/ml each, R&D systems, BBA5) for one hour prior to the adhesion assay.

Techniques: Co-Culture Assay, Cell Adhesion Assay, Expressing, Transduction, Plasmid Preparation, Migration, Cell Culture, Co-culture Assay