Journal: Cell Reports Methods

Article Title: A multi-omics systems vaccinology resource to develop and test computational models of immunity

doi: 10.1016/j.crmeth.2024.100731

Figure Lengend Snippet:

Article Snippet: Anti-Human CCR7 (CyTOF, 167Er, clone G043H7) , Fluidigm , 3167009A; RRID: AB_2858236.

Techniques: Clinical Proteomics, Recombinant, Luminex, Lysis, Software, Derivative Assay

Journal: Scientific Reports

Article Title: Salmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cells

doi: 10.1038/s41598-020-70492-2

Figure Lengend Snippet: Unsupervised clustering of epigenetic changes between and within the different mucosal cell subsets after wild-type S . Typhi infections. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) at 1:100 MOI. Cells cultured with media only were used as controls (media). After 3 h of incubation, cells were stained using a panel of 33 metal-labeled Abs, and the chromatin modifications were analyzed at the single-cell level by mass cytometry. To visualize the clustering of the 11 cell subsets ( i.e ., B-, CD3+ T-, CD4+ T-, CD8 + T-, NK, TCR-γδ, Mucosal associated invariant (MAIT) and NKT cells, as well as monocytes, macrophages, and epithelial cells), tSNE-defined population distributions and clustering were colored by meta-cluster. ( A ) t-SNE maps of chromatin modifications. Settings to run the t-SNE algorithm were set-up in Cytobank. ( B ) Color-coded key showing the location of epigenetic marks meta-clusters. Data are representative of one out of three experiments with terminal ileum segments from 2 different donors, one replicate each.

Article Snippet: Cells were surface stained with anti-human mAbs to CD3 (clone UCHT1), CD4 (clone SK3), CD8 (clone RPA-T8), CD11b (clone ICRF44), CD16 (clone 3G8), CD19 (clone HIB19), CD38 (clone HIT2), CD45 (clone HI30), CD45RO (clone UCHL), CD56 (clone HCD56), CD57 (clone HCD57), CD69 (clone FN50), CD161 (clone HP-3G10), CD163 (clone GHI/61), CCR7 (clone G043H7), EpCAM (CD326, clone 9C4), HLA-DR (clone L243), TCR γδ (clone 11F2)(Fluidigm, Sunnyvale, CA), CD14 (clone 3C10) (Invitrogen, Carlsbad, CA), and TCR Vα7.2 (clone 3C10) (Biolegend, San Diego, CA).

Techniques: Isolation, Cell Culture, Incubation, Staining, Labeling, Mass Cytometry

Journal: Scientific Reports

Article Title: Salmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cells

doi: 10.1038/s41598-020-70492-2

Figure Lengend Snippet: Chromatin profiles of the epigenetic changes in TCR-γδ cells induced by S . Typhi. Cells isolated cells from healthy terminal ileum surgical tissues were exposed to S . Typhi strain Ty2 (Ty2) and cultured as described in Fig. . Cells cultured with media only were used as controls (media). FCOM data of the 28 combinations within the acceptability criteria for changes of the chromatin marks are shown. Bars represent the net difference ( S . Typhi-infected minus uninfected cultures). Bar graphs extend from the 25th to 75th percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. Data are representative of three experiments with terminal ileum segments from 4 different donors, one replicate each. P values < 0.05 were considered significant (red-colored boxes).

Article Snippet: Cells were surface stained with anti-human mAbs to CD3 (clone UCHT1), CD4 (clone SK3), CD8 (clone RPA-T8), CD11b (clone ICRF44), CD16 (clone 3G8), CD19 (clone HIB19), CD38 (clone HIT2), CD45 (clone HI30), CD45RO (clone UCHL), CD56 (clone HCD56), CD57 (clone HCD57), CD69 (clone FN50), CD161 (clone HP-3G10), CD163 (clone GHI/61), CCR7 (clone G043H7), EpCAM (CD326, clone 9C4), HLA-DR (clone L243), TCR γδ (clone 11F2)(Fluidigm, Sunnyvale, CA), CD14 (clone 3C10) (Invitrogen, Carlsbad, CA), and TCR Vα7.2 (clone 3C10) (Biolegend, San Diego, CA).

Techniques: Isolation, Cell Culture, Infection

Journal: Cancers

Article Title: Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

doi: 10.3390/cancers13225693

Figure Lengend Snippet: Representative immunohistochemical staining for CCR7 in the normal adrenal gland (NA). ( A ) 2.5× +, ( B ) 10×, adrenocortical carcinoma, ( C ) 10× +, ( D ) 40×, ( E ) 10× +, ( F ) 40×), aldosterone-producing adenoma (APA), ( G ) 10× +, ( H ) 40×), cortisol-producing adenoma (CPA), ( I ) 10× +, ( J ) 40×, non-functioning adenoma ( K ) 10× +, ( L ) 40×), ( M ) Comparison of membrane staining for CCR7 using H-score (mean ± SD) in ACC ( n = 120), APA ( n = 20), CPA ( n = 18), and NFA ( n = 21). ** p < 0.01, *** p < 0.001 (Dunn’s multiple comparisons test).

Article Snippet: We used the Taqman Gene Expression assays for CCR7 (Hs01013469_m1) from Applied Biosystems (Darmstadt, Germany).

Techniques: Immunohistochemical staining, Staining, Comparison, Membrane

Journal: Cancers

Article Title: Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

doi: 10.3390/cancers13225693

Figure Lengend Snippet: Comparison of membrane staining for CCR7 in adrenocortical carcinoma (primary tumors, n = 120), recurrences ( n = 13), lymph node metastases ( n = 29), and distant metastases ( n = 19) using H-score (mean ± SD). ** p < 0.01, *** p < 0.001 (Dunn’s multiple comparisons test).

Article Snippet: We used the Taqman Gene Expression assays for CCR7 (Hs01013469_m1) from Applied Biosystems (Darmstadt, Germany).

Techniques: Comparison, Membrane, Staining

Journal: Cancers

Article Title: Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

doi: 10.3390/cancers13225693

Figure Lengend Snippet: ( A ) Relative mRNA expression of CCR7 in normal adrenal (NA, n = 4), adrenocortical carcinoma (ACC, n = 9), aldosterone-producing adenoma (APA, n = 11), cortisol-producing adenoma (CPA, n = 10), and non-functioning adenoma (NFA, n = 3). Data is displayed as mean ± SEM. No significant differences were detected (Dunn’s multiple comparisons test). ( B ) mRNA expression data of CCR7 and its ligands CCL19 and CCL21 obtained from publicly available data sets using GEPIA2 ( www.gepia2.cancer-pku.cn/ ; accessed 22 August 2021). Shown are adrenocortical carcinomas (ACC, n = 77) and normal adrenals (NA, n = 128). Significant differences were detected for CCL21 (one-way ANOVA). ( C , D ) Correlations of mRNA expression data for CCR7 and CCL19/21 for ACC and NA, respectively (GEPIA2, www.gepia2.cancer-pku.cn/ , accessed 22 August 2021, Person’s correlation analysis).

Article Snippet: We used the Taqman Gene Expression assays for CCR7 (Hs01013469_m1) from Applied Biosystems (Darmstadt, Germany).

Techniques: Expressing

Journal: Cancers

Article Title: Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

doi: 10.3390/cancers13225693

Figure Lengend Snippet: Comparison of CCR7 ( A ), CCL19 ( B ), and CCL21 ( C ) mRNA expression between CD8+, CD4+, and regulatory T cells, as well as M1 and M2 macrophages using publicly available datasets for adrenocortical carcinoma (ACC) and normal adrenal glands (NA). (GEPIA2021, sub-expression analysis, http://gepia2021.cancer-pku.cn/ , accessed 22 August 2021; CIBERSORT deconvolution tool, one-way ANOVA).

Article Snippet: We used the Taqman Gene Expression assays for CCR7 (Hs01013469_m1) from Applied Biosystems (Darmstadt, Germany).

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

doi: 10.3390/cancers13225693

Figure Lengend Snippet: Progression-free survival ( A ) and overall survival ( B ) of patients with adrenocortical carcinoma influenced by CCR7 H-Score for membrane staining.

Article Snippet: We used the Taqman Gene Expression assays for CCR7 (Hs01013469_m1) from Applied Biosystems (Darmstadt, Germany).

Techniques: Membrane, Staining

Journal: Cancers

Article Title: Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

doi: 10.3390/cancers13225693

Figure Lengend Snippet: Disease free survival ( A ) and overall survival ( B ) of patients with adrenocortical carcinoma based on CCR7 mRNA expression using publicly available data sets. KM-plots were generated using GEPIA2 ( www.gepia2.cancer-pku.cn/ ; accessed 22 August 2021). HR = Hazard Ratio.

Article Snippet: We used the Taqman Gene Expression assays for CCR7 (Hs01013469_m1) from Applied Biosystems (Darmstadt, Germany).

Techniques: Expressing, Generated

Journal: Discover Oncology

Article Title: Integrated WGCNA retrieval of T-cell exhaustion genes related to radiosensitivity in locally advanced cervical cancer and prediction of immunotherapy efficacy

doi: 10.1007/s12672-025-03673-y

Figure Lengend Snippet: IHC assay results of the protein expression levels of APIBEC3H, CCR7, PILRA, and SLC15A3 in LACC patients

Article Snippet: Subsequently, the sections were incubated at 4 °C overnight with the corresponding primary antibody against APOBEC3H (1:200, PA5-54426, Invitrogen, USA), CCR7 (1:100, 25898-1-AP, Proteintech, China), PLRA (1:100, PA5-147474, Invitrogen, USA), SLC15A3 (1:100, PA5-61614, Invitrogen, USA).

Techniques: Expressing