Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

doi: 10.1007/s00018-025-06071-3

Figure Lengend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Article Snippet: The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents.

Techniques: Western Blot, Knockdown, Negative Control

Journal:

Article Title: Influence of fatty acid diets on gene expression in rat mammary epithelial cells

doi: 10.1152/physiolgenomics.00007.2009

Figure Lengend Snippet: Differentially expressed cell cycle genes at PND50 and PND21

Article Snippet: TaqMan Gene Expression Assays were purchased from Applied Biosystems [glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Rn99999916_s1, cyclin-dependent kinase inhibitor 3 predicted (Cdkn3_predicted) Rn01414656_m1, polo-like kinase 1 (Plk1) Rn01510931_m1, aurora kinase B (Aurkb) Rn01460656_ m1, cyclin B1 (Ccnab1) Rn00596848_m1, pituitary tumor-transforming 1 (Pttg1) Rn00574373_m1, M-phase phosphoprotein 1 predicted (Mphosph1_predicted) Rn01454431_m1, Ect2 oncogene predicted (Ect2_predicted) Rn01457088_m1, cyclin A2 (Ccna2) Rn01493715_m1, and E2F transcription factor 8 (E2f8) Rn01476919_m1].

Techniques:

Journal: BMC Genomics

Article Title: Transcriptome analysis reveals differences in mechanisms regulating cessation of luteal function in pregnant and non-pregnant dogs

doi: 10.1186/s12864-017-4084-9

Figure Lengend Snippet: List of primers used for Real Time (TaqMan) PCR

Article Snippet: CCNA2 , XM_540965.2 , Applied Biosystems, prod. nr.: Cf02648449_g1 , 77.

Techniques: Sequencing