Journal: Cancer Cell International

Article Title: PIBF1 (p.R405Q) germline variant identified in cancer susceptibility family impairs protein stability and function

doi: 10.1186/s12935-025-04132-y

Figure Lengend Snippet: PIBF1 (p.R405Q) mutation is a candidate gene for hereditary cancer syndromes. A . The expression of PIBF1 is low in BRCA patients in the UALCAN database. B . The expression level of PIBF1 in the UALCAN database is negatively correlated with the tumor grade. C . The Kaplan-Meier Plotter plots survival curves of PIBF1 and low expression of PIBF1 was correlated with poor prognosis of breast cancer. D , E . Q-PCR and WB detection of PIBF1-WT and PIBF1(p.R405Q) in the MDA-MB-231 and MDA-MB-468. F . CCK8 was performed to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the cell growth of breast cancer cells. G , H . Plate cloning assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the proliferation of breast cancer cells. I . Transwell assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the invasion ability of breast cancer cells. J . WB assay to detect the regulatory effect of PIBF1-WT and PIBF1(p.R405Q) on ERK phosphorylation. All data are presented as mean ± SEM ( n = 3 independent cell culture experiments), *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: The medium was refreshed every 24 h with a 1:10 dilution of CCK8 reagent (Cat#C0005, TargetMol, USA) and incubated at 37 °C for 3 hours before measuring absorbance at 450 nm using a microplate reader (EnSpire 2300, PerkinElmer, USA).

Techniques: Mutagenesis, Expressing, Cloning, Transwell Assay, Phospho-proteomics, Cell Culture

Journal: Journal of Oncology

Article Title: The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma

doi: 10.1155/2021/9958483

Figure Lengend Snippet: The impact of PC4 on the malignant phenotypes of lung adenocarcinoma cells. (a), (b) Western blot assay revealed the protein levels of PC4 after LUAD cells were infected with PC4-knockdown or PC4-overexpression lentivirus. (c) The apoptosis of PC4-knockdown or PC4-overexpression LUAD cells was evaluated by flow cytometry. (d) The growth of PC4-knockdown or PC4-overexpression LUAD cells was analyzed via CCK-8 experiments. (e) The migration of LUAD cells following PC4 knockdown or overexpression was detected via Transwell assay. ∗ p < 0.05.

Article Snippet: The CCK-8 test was accomplished using the Cell Counting Kit-8 (CCK-8) (Bioss, China) as we previously described [ ].

Techniques: Western Blot, Infection, Over Expression, Flow Cytometry, CCK-8 Assay, Migration, Transwell Assay

Journal: Journal of Oncology

Article Title: The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma

doi: 10.1155/2021/9958483

Figure Lengend Snippet: PC4 decreases cisplatin's cytotoxic effects on lung adenocarcinoma cells in vitro . (a) PC4-knockdown or PC4-overexpression LUAD cells underwent 24-hour treatment with 20 μ M cisplatin. The expression of apoptotic proteins was evaluated. (b) PC4-knockdown or PC4-overexpression LUAD cells were administrated with 20 µ M cisplatin for 96 hours. The CCK-8 assay was performed every 24 hours. (c), (d) PC4-knockdown or PC4-overexpression LUAD cells underwent 24-hour treatment with 0, 20, and 30 µ M cisplatin. Cell apoptosis was measured and analyzed by flow cytometry. ∗ p < 0.05.

Article Snippet: The CCK-8 test was accomplished using the Cell Counting Kit-8 (CCK-8) (Bioss, China) as we previously described [ ].

Techniques: In Vitro, Over Expression, Expressing, CCK-8 Assay, Flow Cytometry