Journal: Cell Death & Disease

Article Title: A novel lncRNA n384546 promotes thyroid papillary cancer progression and metastasis by acting as a competing endogenous RNA of miR-145-5p to regulate AKT3

doi: 10.1038/s41419-019-1637-7

Figure Lengend Snippet: a Validation of Gapmer-n384546 knockdown efficiency in B-CPAP and KTC-1 cells was determined by qRT-PCR. b CCK-8 proliferation assay in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. c Colony formation assay in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. d EdU proliferation assay in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. e Flow cytometric analysis of apoptosis in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. f Tumor size and tumor weight of nude mice was measured and analyzed. g Tumor volume curves of nude mice injected with sh-control and sh-n384546 B-CPAP cells were analyzed. h n384546 expression in tumors collected from nude mice was determined by qRT-PCR. i Immunohistochemical staining of Bcl-2, caspase9 and Ki-67 was used to assess proliferation and apoptosis (400×). Data represent the mean ± SEM of three separate experiments. All experiments were repeated at least three times. * p < 0.05, ** p < 0.01 in independent Student’s t test

Article Snippet: Cell proliferation and viability were analyzed using CCK8 (Cell Counting Kit-8, Dojindo, Japan) following the protocol provided by the manufacturer.

Techniques: Biomarker Discovery, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Proliferation Assay, Transfection, Colony Assay, Injection, Control, Expressing, Immunohistochemical staining, Staining

Journal: Cell Death & Disease

Article Title: A novel lncRNA n384546 promotes thyroid papillary cancer progression and metastasis by acting as a competing endogenous RNA of miR-145-5p to regulate AKT3

doi: 10.1038/s41419-019-1637-7

Figure Lengend Snippet: a CCK-8 proliferation assay, b EdU proliferation assay, c Flow cytometric analysis of apoptosis, d Transwell invasion assay, e Transwell migration assay, and f Wound healing assay were performed in B-CPAP and KTC-1 cells transfected with Scrambled Gapmer, anti-miR-145, mimic-miR-145, Gapmer-n384546, Gapmer-n384546 + anti-miR-145, Gapmer-n384546 + mimic-miR-145. g The expression of proteins in B-CPAP cells transfected with Scrambled Gapmer, anti-miR-145, mimic-miR-145, Gapmer-n384546, Gapmer-n384546 + anti-miR-145, Gapmer-n384546 + mimic-miR-145 was determined by western blot. Data represent the mean ± SEM of three separate experiments. All experiments were repeated at least three times. * p < 0.05, ** p < 0.01 in independent Student’s t test ( a – f )

Article Snippet: Cell proliferation and viability were analyzed using CCK8 (Cell Counting Kit-8, Dojindo, Japan) following the protocol provided by the manufacturer.

Techniques: CCK-8 Assay, Proliferation Assay, Transwell Invasion Assay, Transwell Migration Assay, Wound Healing Assay, Transfection, Expressing, Western Blot

Journal: Age and ageing

Article Title: Moderate physical activity associated with a higher naïve/memory T-cell ratio in healthy old individuals: potential role of IL15.

doi: 10.1093/ageing/afaa035

Figure Lengend Snippet: Figure 1. Impact of physical activity on T-cell subset distribution, thymic output and B-cell subset distribution. Percentage of (a) naïve CD4 T cells, (b) memory CD4 T cells in sedentary (n = 25) and physically active healthy older participants (n = 25). (c) Association between serum IL-15 levels and peripheral frequency of naïve CD4 T cells (n = 50). Percentage of (d) PTK7+ve

Article Snippet: For phenotypic characterisation of T-cell subset distribution, PBMCs (1 × 106/ml) were stained with a combination of fluorochrome-conjugated antibodies, anti-human CD3-PEcy7 (eBiosciences, clone UCHT1), anti-human CD4 Violet (eBiosciences, clone RPA-T4), anti-human CD8 PE (Immunotools, clone UCHT4), antihuman CCR7 FITC (R and D systems, clone 150,503), anti-human CD45RA APC (Biolegend, clone HI-100) and anti-human PTK7 (Miltenyi Biotech, clone 188B).

Techniques: Activity Assay

Journal: bioRxiv

Article Title: ADAM10 tailors extracellular vesicles for content transfer rather than signaling by contact

doi: 10.64898/2026.02.12.705562

Figure Lengend Snippet: A. Summary table presenting the proteins identified and quantified by mass spectrometry analysis of ADAM10-KO cell lysates compared to control MCF7 cells and their corresponding secreted sEVs. B-C. sEVs secreted by MCF7 cells with knock-out of ADAM10 (A10 KO) or control cells (WT) were isolated by differential ultracentrifugation of conditioned culture media. Cell lysates and sEVs were analyzed by Western blot, testing for several proteins including EphrinB3, BCAM, E-cadherin and PTK7 ( B ), SRC ( C ). Histograms represent mean signal intensities normalized to controls ± SEM, calculated from n independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n.s. non-significant (multiple unpaired t-test).

Article Snippet: Antibodies directed against SDC1 intracellular domain (D4Y7H) was from (cell signaling #12922, dilution 1/1000), GFP (A11122) from Thermofisher (dilution 1/1000), ADAM17 (abcam, #ab39162, 1/1000 or cell signaling #3976, 1/1000), ADAM10 (abcam, #ab1997, 1/1 000), GAPDH (Proteintech # 10494-1-AP), Flotillin-1 (BD Biosciences, #X11669), Ephrin B3 (santa cruz, # sc-514139), PTK7 (Proteintech, #17799), E-cadherin (R&D Systems, #AF748), BCAM (R&D Systems, #BAF148), STAT3 124H6 (cell signaling #9139), p-STAT3 (cell signaling #9131).

Techniques: Mass Spectrometry, Control, Knock-Out, Isolation, Western Blot