Journal: Pediatric Research

Article Title: microRNA profilings identify plasma biomarkers and targets associated with pediatric epilepsy patients

doi: 10.1038/s41390-023-02864-z

Figure Lengend Snippet: Novel identified target genes of miR-27a-3p from our mRNA-seq screening ( p < 0.05).

Article Snippet: Antibodies included GOLM1 (1:4000; Proteintech, Cat# 15126-1-AP), LIMK1 (1:1000; affinity, Cat#AF6345), CBX1 (1:1000; Proteintech, Cat#10241-2-AP), and GAPDH (1:5000; Proteintech, Cat# 60004-1-Ig).

Techniques: Over Expression, Sequencing

Journal: Pediatric Research

Article Title: microRNA profilings identify plasma biomarkers and targets associated with pediatric epilepsy patients

doi: 10.1038/s41390-023-02864-z

Figure Lengend Snippet: Data are presented as mean ± SEM ( n = 3), * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the control group. a The mRNA expression levels of GOLM1, LIMK1, and CBX1 in HEK-293T cells after overexpression of miR-27a-3p, with β-actin used as an internal reference standard. b Quantitative analysis of GOLM1, LIMK1, and p-LIMK1 protein levels in HEK293 cells (normalized to GAPDH). Representative WB results are shown. c The expression levels of miR-27a-3p in mouse plasma were determined 1 h after epilepsy was terminated, with the external standard used as a reference control; the expression level of miR-27a-3p in mouse brain 6 h after epilepsy was terminated, with U6 used as a reference. The expression of miR-27a-3p in plasma and whole brain in the epilepsy group was upregulated. d RNA expression levels of GOLM1 and LIMK1 in the whole brain of mice 6 h after epilepsy was terminated (GAPDH was used as an internal reference for GOLM1 and LIMK1). e Representative immunofluorescence staining images of GOLM1, LIMK1, and P-LIMK1 (green) in mouse hippocampus 6 h after epilepsy termination. Staining levels of GOLM1, LIMK1, and P-LIMK1 were reduced in the epilepsy group (brain scale bars, 500 μm; hippocampal scale bars, 100 μm). f Quantification data of mean fluorescence intensity of mouse hippocampal CA1, CA3, DG region of GOLM1 ( n = 3), LIMK1 ( n = 3), and P-LIMK1 ( n = 3). Data are presented as mean ± SEM; * p < 0.05 and ** p < 0.01 vs. the control group.

Article Snippet: Antibodies included GOLM1 (1:4000; Proteintech, Cat# 15126-1-AP), LIMK1 (1:1000; affinity, Cat#AF6345), CBX1 (1:1000; Proteintech, Cat#10241-2-AP), and GAPDH (1:5000; Proteintech, Cat# 60004-1-Ig).

Techniques: Control, Expressing, Over Expression, Clinical Proteomics, RNA Expression, Immunofluorescence, Staining, Fluorescence