cba Search Results


95
Chem Impex International buffer components
Buffer Components, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation cytokine bead assay cba kits
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Cytokine Bead Assay Cba Kits, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tocris drug cba tocris
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Drug Cba Tocris, supplied by Tocris, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc sgrna plasmid targeting lamin a
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Sgrna Plasmid Targeting Lamin A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc egfp t2a sequence
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Egfp T2a Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc hm4di
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Hm4di, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Envigo cba j mice
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Cba J Mice, supplied by Envigo, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc jrcamp1b
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Jrcamp1b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chem Impex International w linkers 2 4 formylphenoxy acetic acid
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
W Linkers 2 4 Formylphenoxy Acetic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc aav injection procedure
Multi-specific CD4 + and CD8 + T cell intracellular <t>cytokine</t> responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Aav Injection Procedure, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc id 110135
Key Resources Table
Id 110135, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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KCAS Bioanalytical and Biomarker Services kcas bio analytical
Key Resources Table
Kcas Bio Analytical, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Multi-specific CD4 + and CD8 + T cell intracellular cytokine responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Multi-specific CD4 + and CD8 + T cell intracellular cytokine responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Derivative Assay, Incubation, Negative Control, Membrane

Differential T cells responses, pursuant to the number and type of cytokine produced within the different cohorts. (A) CD4 + and (B) CD8 + T cells producing cytokines after stimulation with the four peptide pools derived from SARs-CoV-2. The pie charts represent the proportion of subjects producing a different number of cytokines per group in response to each of the four peptide pools; the arcs show the production of each analysed cytokine, each corresponding to a different colour as indicated. (M, membrane; NP, nucleoprotein; ORF-7a-2, open reading frame 7a-2; S, spike). (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Differential T cells responses, pursuant to the number and type of cytokine produced within the different cohorts. (A) CD4 + and (B) CD8 + T cells producing cytokines after stimulation with the four peptide pools derived from SARs-CoV-2. The pie charts represent the proportion of subjects producing a different number of cytokines per group in response to each of the four peptide pools; the arcs show the production of each analysed cytokine, each corresponding to a different colour as indicated. (M, membrane; NP, nucleoprotein; ORF-7a-2, open reading frame 7a-2; S, spike). (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Produced, Derivative Assay, Membrane

Distinct serum cytokine profiles in the subject cohorts and relative to antibody status. (A) Heatmap showing differential serum cytokine expression in the study subjects listed accordingly to respective anti-nucleoprotein IgG levels (Architect Index). (B) β-NGF and (C) IL-1α serum levels within the 3 different subject cohorts; boxes extend from the 25th to 75th percentiles, the line indicates the median, and the whiskers extend from the smaller to the largest values. Correlative expression of β-NGF with respect to (D) Architect index and (E) nAb levels, within the whole cohort. p-values in (B, C) were determined by a one-way ANOVA with a Tukey’s post-hoc test for multiple comparisons. A Spearman non-parametric correlation test was undertaken to test significance in (D, E) . *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Distinct serum cytokine profiles in the subject cohorts and relative to antibody status. (A) Heatmap showing differential serum cytokine expression in the study subjects listed accordingly to respective anti-nucleoprotein IgG levels (Architect Index). (B) β-NGF and (C) IL-1α serum levels within the 3 different subject cohorts; boxes extend from the 25th to 75th percentiles, the line indicates the median, and the whiskers extend from the smaller to the largest values. Correlative expression of β-NGF with respect to (D) Architect index and (E) nAb levels, within the whole cohort. p-values in (B, C) were determined by a one-way ANOVA with a Tukey’s post-hoc test for multiple comparisons. A Spearman non-parametric correlation test was undertaken to test significance in (D, E) . *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Expressing

Pearson correlation test between the Architect Index and serum cytokines levels.

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Pearson correlation test between the Architect Index and serum cytokines levels.

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques:

Global and antigen specific T cell expression of the β-NGF receptor TrkA, from the different cohorts. Correlative expression of β-NGF against MFI of TrkA + (A) CD4 + and (B) CD8 + T cells from all subjects studied. Summary data of percentage TrkA + (C) CD4 + and (D) CD8 + T cells (top), with representative FACS plots (bottom) from each cohort. Summary data of MFI expression of TrkA on (E) CD4 + and (F) CD8 + T cells (top); with representative MFI histograms from each cohort (bottom). MFI of TrkA + (G) CD4 + and (H) CD8 + T cells producing the respective cytokines after 16-hour stimulation with the four peptide pools derived from SARS-CoV-2. A Spearman non-parametric correlation test was undertaken to test significance in (A, B) , a one-ANOVA (C–F) and a two-way ANOVA (G, H) with a Tukey’s post-hoc test for multiple comparisons was used to demonstrate significance. Coloured lines for significance indicate changes relative to the corresponding cytokine. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Global and antigen specific T cell expression of the β-NGF receptor TrkA, from the different cohorts. Correlative expression of β-NGF against MFI of TrkA + (A) CD4 + and (B) CD8 + T cells from all subjects studied. Summary data of percentage TrkA + (C) CD4 + and (D) CD8 + T cells (top), with representative FACS plots (bottom) from each cohort. Summary data of MFI expression of TrkA on (E) CD4 + and (F) CD8 + T cells (top); with representative MFI histograms from each cohort (bottom). MFI of TrkA + (G) CD4 + and (H) CD8 + T cells producing the respective cytokines after 16-hour stimulation with the four peptide pools derived from SARS-CoV-2. A Spearman non-parametric correlation test was undertaken to test significance in (A, B) , a one-ANOVA (C–F) and a two-way ANOVA (G, H) with a Tukey’s post-hoc test for multiple comparisons was used to demonstrate significance. Coloured lines for significance indicate changes relative to the corresponding cytokine. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Expressing, Derivative Assay

Key Resources Table

Journal: Cell

Article Title: The striatum organizes 3D behavior via moment-to-moment action selection

doi: 10.1016/j.cell.2018.04.019

Figure Lengend Snippet: Key Resources Table

Article Snippet: pAAV-CBA-do(Fas)-GCaMP6s , This paper , Addgene ID 110135.

Techniques: Plasmid Preparation, Recombinant, Software