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Image Search Results
Journal: Frontiers in Immunology
Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19
doi: 10.3389/fimmu.2021.813300
Figure Lengend Snippet: Multi-specific CD4 + and CD8 + T cell intracellular cytokine responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using
Techniques: Derivative Assay, Incubation, Negative Control, Membrane
Journal: Frontiers in Immunology
Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19
doi: 10.3389/fimmu.2021.813300
Figure Lengend Snippet: Differential T cells responses, pursuant to the number and type of cytokine produced within the different cohorts. (A) CD4 + and (B) CD8 + T cells producing cytokines after stimulation with the four peptide pools derived from SARs-CoV-2. The pie charts represent the proportion of subjects producing a different number of cytokines per group in response to each of the four peptide pools; the arcs show the production of each analysed cytokine, each corresponding to a different colour as indicated. (M, membrane; NP, nucleoprotein; ORF-7a-2, open reading frame 7a-2; S, spike). (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).
Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using
Techniques: Produced, Derivative Assay, Membrane
Journal: Frontiers in Immunology
Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19
doi: 10.3389/fimmu.2021.813300
Figure Lengend Snippet: Distinct serum cytokine profiles in the subject cohorts and relative to antibody status. (A) Heatmap showing differential serum cytokine expression in the study subjects listed accordingly to respective anti-nucleoprotein IgG levels (Architect Index). (B) β-NGF and (C) IL-1α serum levels within the 3 different subject cohorts; boxes extend from the 25th to 75th percentiles, the line indicates the median, and the whiskers extend from the smaller to the largest values. Correlative expression of β-NGF with respect to (D) Architect index and (E) nAb levels, within the whole cohort. p-values in (B, C) were determined by a one-way ANOVA with a Tukey’s post-hoc test for multiple comparisons. A Spearman non-parametric correlation test was undertaken to test significance in (D, E) . *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).
Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using
Techniques: Expressing
Journal: Frontiers in Immunology
Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19
doi: 10.3389/fimmu.2021.813300
Figure Lengend Snippet: Pearson correlation test between the Architect Index and serum cytokines levels.
Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using
Techniques:
Journal: Frontiers in Immunology
Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19
doi: 10.3389/fimmu.2021.813300
Figure Lengend Snippet: Global and antigen specific T cell expression of the β-NGF receptor TrkA, from the different cohorts. Correlative expression of β-NGF against MFI of TrkA + (A) CD4 + and (B) CD8 + T cells from all subjects studied. Summary data of percentage TrkA + (C) CD4 + and (D) CD8 + T cells (top), with representative FACS plots (bottom) from each cohort. Summary data of MFI expression of TrkA on (E) CD4 + and (F) CD8 + T cells (top); with representative MFI histograms from each cohort (bottom). MFI of TrkA + (G) CD4 + and (H) CD8 + T cells producing the respective cytokines after 16-hour stimulation with the four peptide pools derived from SARS-CoV-2. A Spearman non-parametric correlation test was undertaken to test significance in (A, B) , a one-ANOVA (C–F) and a two-way ANOVA (G, H) with a Tukey’s post-hoc test for multiple comparisons was used to demonstrate significance. Coloured lines for significance indicate changes relative to the corresponding cytokine. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).
Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using
Techniques: Expressing, Derivative Assay
Journal: Cell
Article Title: The striatum organizes 3D behavior via moment-to-moment action selection
doi: 10.1016/j.cell.2018.04.019
Figure Lengend Snippet: Key Resources Table
Article Snippet: pAAV-CBA-do(Fas)-GCaMP6s , This paper ,
Techniques: Plasmid Preparation, Recombinant, Software