cb839 Search Results


cb839  (Tocris)
94
Tocris cb839
Cb839, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
cb839 - by Bioz Stars, 2026-04
94/100 stars
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telaglenastat  (MedChemExpress)
96
MedChemExpress telaglenastat
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Telaglenastat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
telaglenastat - by Bioz Stars, 2026-04
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cb 839  (TargetMol)
94
TargetMol cb 839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cb 839/product/TargetMol
Average 94 stars, based on 1 article reviews
cb 839 - by Bioz Stars, 2026-04
94/100 stars
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kga inhibitor glutaminase in  (MedChemExpress)
93
MedChemExpress kga inhibitor glutaminase in
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Kga Inhibitor Glutaminase In, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
kga inhibitor glutaminase in - by Bioz Stars, 2026-04
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cb 839  (BOC Sciences)
90
BOC Sciences cb 839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cb 839 - by Bioz Stars, 2026-04
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cb-839  (Calithera inc)
90
Calithera inc cb-839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by Calithera inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cb-839/product/Calithera inc
Average 90 stars, based on 1 article reviews
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cb-839, an inhibitor of glutaminase  (Cayman Chemical)
90
Cayman Chemical cb-839, an inhibitor of glutaminase
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, An Inhibitor Of Glutaminase, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cb-839, an inhibitor of glutaminase/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
cb-839, an inhibitor of glutaminase - by Bioz Stars, 2026-04
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cb-839  (Merck KGaA)
90
Merck KGaA cb-839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cb-839/product/Merck KGaA
Average 90 stars, based on 1 article reviews
cb-839 - by Bioz Stars, 2026-04
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cb-839  (Incozen Therapeutics Pvt Ltd)
90
Incozen Therapeutics Pvt Ltd cb-839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by Incozen Therapeutics Pvt Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cb-839/product/Incozen Therapeutics Pvt Ltd
Average 90 stars, based on 1 article reviews
cb-839 - by Bioz Stars, 2026-04
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cb 839  (Focus Biomolecules)
90
Focus Biomolecules cb 839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by Focus Biomolecules, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cb 839/product/Focus Biomolecules
Average 90 stars, based on 1 article reviews
cb 839 - by Bioz Stars, 2026-04
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cb-839  (BLDpharm)
90
BLDpharm cb-839
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839, supplied by BLDpharm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cb-839 - by Bioz Stars, 2026-04
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cb-839 drug  (research diets inc)
90
research diets inc cb-839 drug
A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or <t>Telaglenastat).</t> Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.
Cb 839 Drug, supplied by research diets inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or Telaglenastat). Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.

Journal: bioRxiv

Article Title: Metabolic reprogramming controlled by NF-YA alternative splicing creates therapeutic opportunities in colorectal cancer

doi: 10.64898/2026.03.03.708979

Figure Lengend Snippet: A) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium or in Gln-deprived medium + 2 mM Glutamine. Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and represent mean±SEM (one-sample t-test for comparison with control medium (§); one-way ANOVA with Fisher’s LSD test for comparison between samples (∗); n=4). B) Cell viability of Empty, NF-YAl high , and NF-YAs high HCT116 cells analyzed by PrestoBlue cell viability assay after 72h in Gln-deprived medium either w/o or with inhibitors (Glufosinate, V-9302 or Telaglenastat). Data were normalized to cell growth in complete CTR medium, arbitrarily set at 100% (dotted line), and presented as mean±SEM (two-way ANOVA with Fisher’s LSD test for comparison between samples; 3≤n≤6). C) Representative images of cell migration in wound-healing assay. Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 24h, and 48h after the culture chambers were removed. D) Quantification of migration in Ibidi wound-healing chambers is shown as wound closure (%) compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test; n=3). E) Optical microscopy images representative of spheroid-based metastatic potential assay. Two spheroids generated from Empty, NF-YAl high , and NF-YAs high HCT116 were transferred into tissue-culture 48-well plates and then grown in complete IMDM medium (CTR) or medium without Gln ± 10 μg/ml Glufosinate. Images were taken after 10 days to determine whether the cells were able to move from spheroids and establish colonies. Arrows indicate representative colonies.

Article Snippet: When indicated, we concomitantly added Gln pathways inhibitors: 10 μg/ml Glufosinate-ammonium (Sigma-Aldrich, #45520), 1μM V-9302 (MedChemExpress, #HY-112683A) or 25nM Telaglenastat -CB-839-(MedChemExpress, #HY-12248).

Techniques: Viability Assay, Comparison, Control, Migration, Wound Healing Assay, Microscopy, Generated