cav3 Search Results


cav3 3  (Alomone Labs)
94
Alomone Labs cav3 3
Cav3 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Anti-CACNA1G+(CaV3%2E1)+Antibody/10__1590_slash_1414___431x2023e11879-52-4-10
Average 94 stars, based on 1 article reviews
cav3 3 - by Bioz Stars, 2026-09
94/100 stars
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mouse anti ca v 3 2  (Novus Biologicals)
94
Novus Biologicals mouse anti ca v 3 2
Mouse Anti Ca V 3 2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Cav3%2E2+Antibody+(S55%2F10)/pmc11940306-75-9-15
Average 94 stars, based on 1 article reviews
mouse anti ca v 3 2 - by Bioz Stars, 2026-09
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anti cav3 2  (Alomone Labs)
94
Alomone Labs anti cav3 2
Anti Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Anti-CaV3%2E2+(CACNA1H)+Antibody/pmc10423893-88-44-48
Average 94 stars, based on 1 article reviews
anti cav3 2 - by Bioz Stars, 2026-09
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cav3 2 antibody  (Proteintech)
93
Proteintech cav3 2 antibody
Cav3 2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Caveolin-3+Antibody/pmc08369173-70-31-38
Average 93 stars, based on 1 article reviews
cav3 2 antibody - by Bioz Stars, 2026-09
93/100 stars
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mouse monoclonal igg1 anti cav3 1  (NeuroMab)
93
NeuroMab mouse monoclonal igg1 anti cav3 1
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Mouse Monoclonal Igg1 Anti Cav3 1, supplied by NeuroMab, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Anti-Cav3%2E1+Ca2%2B+Channel+Antibody/pmc07408500-12-0-5
Average 93 stars, based on 1 article reviews
mouse monoclonal igg1 anti cav3 1 - by Bioz Stars, 2026-09
93/100 stars
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rabbit anti ca v 3 3 polyclonal antibody  (Alomone Labs)
94
Alomone Labs rabbit anti ca v 3 3 polyclonal antibody
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Rabbit Anti Ca V 3 3 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Anti-CaV3%2E3+(CACNA1I)+Antibody/pmc10075096-76-52-58
Average 94 stars, based on 1 article reviews
rabbit anti ca v 3 3 polyclonal antibody - by Bioz Stars, 2026-09
94/100 stars
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anti ca v 3 2 mouse monoclonal antibody  (NeuroMab)
90
NeuroMab anti ca v 3 2 mouse monoclonal antibody
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Anti Ca V 3 2 Mouse Monoclonal Antibody, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Anti-Cav3%2E2+Ca2%2B+Channel+Antibody/pmc05786188-226-1-10
Average 90 stars, based on 1 article reviews
anti ca v 3 2 mouse monoclonal antibody - by Bioz Stars, 2026-09
90/100 stars
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nbp1 22444  (Novus Biologicals)
93
Novus Biologicals nbp1 22444
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Nbp1 22444, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Cav3%2E2+Antibody+(S55%2F10)/pmc07189999-182-0-4
Average 93 stars, based on 1 article reviews
nbp1 22444 - by Bioz Stars, 2026-09
93/100 stars
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human caveolin 3 cav 3  (OriGene)
90
OriGene human caveolin 3 cav 3
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Human Caveolin 3 Cav 3, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Caveolin+3+(CAV3)+Human+qPCR+Template+Standard/10__1074_slash_jbc__m112__377085-53-7-13
Average 90 stars, based on 1 article reviews
human caveolin 3 cav 3 - by Bioz Stars, 2026-09
90/100 stars
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anti cav3 2  (Novus Biologicals)
90
Novus Biologicals anti cav3 2
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Anti Cav3 2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Cav3%2E2+Antibody/pm23486206-76-17-18
Average 90 stars, based on 1 article reviews
anti cav3 2 - by Bioz Stars, 2026-09
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cav 3  (Cyagen Biosciences)
94
Cyagen Biosciences cav 3
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Cav 3, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Cav3/10__1016_slash_j__smhs__2026__03__004-34-17-34
Average 94 stars, based on 1 article reviews
cav 3 - by Bioz Stars, 2026-09
94/100 stars
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anti cav3 2 antibody  (Novus Biologicals)
92
Novus Biologicals anti cav3 2 antibody
Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal <t>IgG1;</t> middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.
Anti Cav3 2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav3/Cav3%2E2+Antibody/pm38325845-57-20-22
Average 92 stars, based on 1 article reviews
anti cav3 2 antibody - by Bioz Stars, 2026-09
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Image Search Results


Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal IgG1; middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.

Journal: Cell Reports

Article Title: A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses

doi: 10.1016/j.celrep.2020.107968

Figure Lengend Snippet: Multiplexed Immunolabeling in Epoxy-Resin-Embedded Tissue after Etching and Antigen Retrieval (A) Multiple immunolabeling of an epoxy-resin-embedded section of the hippocampal CA1 area of an adult rat. Four rounds of triple labeling were performed using primary Abs raised in 3 different species (top row: mouse monoclonal IgG1; middle: rabbit polyclonal; bottom: Guinea pig polyclonal Abs). Reactions within one round were visualized with Alexa488-, Cy3-, and Cy5-coupled sAbs (all reactions are pseudocolored and shown in cyan). Boxed areas are shown at higher magnifications in panels (B 1 and B 2 ). (B 1 ) Immunolabeling for PV, Kv2.1, VGAT, and vGluT1 are present in different, nonoverlapping subcellular compartments. The vGluT1-positive terminals are only present around the PV+ IN soma but not the neighboring PC somata that are outlined by the Kv2.1 labeling. VGAT-positive terminals (cyan) surround the somata of the IN and the PCs. (B 2 ) Immunolabeling of PV, PSD95, CB1, and neuroligin-2 (NL-2). (C) Confocal images of a 200-nm-thick section of the mouse hilar region labeled for PSD95 (1 st and 3 rd rounds) and for VGAT (2 nd and 4 th rounds; both Guinea pig primary Abs). Circular ROIs were placed over PSD95-labeled glutamatergic synapses (yellow) and over VGAT-positive axons (cyan). The nonspecific background labeling (white ROI) was measured over the unlabeled neuropil. (D) Mean normalized integrated fluorescence values are plotted for 4 labeling rounds. Open symbols represent reactions from 3 mice, and the filled symbols are mean ± SD. The fluorescence in the inhibitory axons is 2.6% ± 2.4% in the 1 st and 4.6% ± 2.5% in the 3 rd rounds, similar to that measured over the excitatory synapses in the 2 nd (7.2% ± 1.1%) and 4 th (5.7% ± 2.9%) rounds. A total of 21–28 ROIs were analyzed in each condition. str. rad., stratum radiatum; str. pyr., stratum pyramidale.

Article Snippet: Mouse monoclonal IgG1 anti-Cav3.1 , NeuroMab , Cat#75-206; RRID: AB_2069421.

Techniques: Immunolabeling, Labeling, Fluorescence

Journal: Cell Reports

Article Title: A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses

doi: 10.1016/j.celrep.2020.107968

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal IgG1 anti-Cav3.1 , NeuroMab , Cat#75-206; RRID: AB_2069421.

Techniques: Virus, Plasmid Preparation, Recombinant, Software, Microscopy