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Image Search Results
Journal: OncoTargets and Therapy
Article Title: Aloperine Induces Ferroptosis of Colorectal Cancer Cells via the Nrf2 Pathway
doi: 10.2147/OTT.S575500
Figure Lengend Snippet: ALO induces ferroptosis in CRC cells by inhibiting the Nrf2 pathway The binding conformation between ALO and the Nrf2 receptor was analyzed using AutoDock Tools. The lowest binding energy conformation was identified at the ALA-510 binding site, which interacts via a hydrogen bond with a length of 1.9 ( A ). Potential active pockets of ALO in the Nrf2 protein were evaluated using CavityPlus software; the GLY364-ALA670 domain was identified as the active binding pocket of Nrf2, and ALO bound to this pocket as expected ( B ). After treating HCT116 and SW480 cells with ALO (0 µM, 200 µM and 300 µM) for 24 hours, immunofluorescence was used to detect intracellular Nrf2 changes in HCT116 ( C and D ) and SW480 ( E and F ) cells treated with different concentrations of ALO. Western Blotting was used to detect the protein expression levels of Nrf2, DMT1, xCT and GPX4 ( G – J ). The data were expressed as mean ± SD (n = 3). * p < 0.05, compared with the control group (0 µM); # p < 0.05, compared with the 200 µM treatment group.
Article Snippet:
Techniques: Binding Assay, Software, Immunofluorescence, Western Blot, Expressing, Control