catalase Search Results


catalase  (Athens Research)
93
Athens Research catalase
Catalase, supplied by Athens Research, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Catalase/pmc00033250-132-3-7
Average 93 stars, based on 1 article reviews
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catalase assay kit  (Elabscience Biotechnology)
96
Elabscience Biotechnology catalase assay kit
Catalase Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Catalase+(CAT)+Activity+Assay+Kit/10__9734_slash_jsrr_slash_2022_slash_v28i121718-88-17-20
Average 96 stars, based on 1 article reviews
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catalase human sirna oligo duplex  (OriGene)
90
OriGene catalase human sirna oligo duplex
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Catalase Human Sirna Oligo Duplex, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Catalase+(CAT)+Human+siRNA+Oligo+Duplex/pm35378363-92-5-13
Average 90 stars, based on 1 article reviews
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catalase primary antibody  (R&D Systems)
94
R&D Systems catalase primary antibody
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Catalase Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Human%2FMouse%2FRat+Catalase+Antibody/pmc12721483-121-14-19
Average 94 stars, based on 1 article reviews
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catalase  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc catalase
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Catalase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Catalase+XP+Rabbit+mAb/pmc12183182-30-21-35
Average 94 stars, based on 1 article reviews
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94/100 stars
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human catalase cat elisa kit  (Cusabio)
93
Cusabio human catalase cat elisa kit
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Human Catalase Cat Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Human+Catalase+(CAT)+ELISA+kit/pm37571262-110-37-42
Average 93 stars, based on 1 article reviews
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cat  (Proteintech)
96
Proteintech cat
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Cat, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Catalase+Antibody/pm36786954-100-65-68
Average 96 stars, based on 1 article reviews
cat - by Bioz Stars, 2026-10
96/100 stars
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rabbit anti catalase  (Rockland Immunochemicals)
94
Rockland Immunochemicals rabbit anti catalase
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Rabbit Anti Catalase, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/Anti-Catalase+rabbit/10__1074_slash_jbc__m800600200-53-42-44
Average 94 stars, based on 1 article reviews
rabbit anti catalase - by Bioz Stars, 2026-10
94/100 stars
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anti catalase  (Rockland Immunochemicals)
94
Rockland Immunochemicals anti catalase
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Anti Catalase, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/CATALASE+ANTIBODY/10__1074_slash_jbc__m600536200-99-118-119
Average 94 stars, based on 1 article reviews
anti catalase - by Bioz Stars, 2026-10
94/100 stars
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catalase  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology catalase
Fig. 8. <t>siRNA-mediated</t> knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).
Catalase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalase/catalase+Antibody/10__1016_slash_j__jff__2015__09__010-49-7-16
Average 96 stars, based on 1 article reviews
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Image Search Results


Fig. 8. siRNA-mediated knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).

Journal: Redox biology

Article Title: Dietary salt initiates redox signaling between endothelium and vascular smooth muscle through NADPH oxidase 4.

doi: 10.1016/j.redox.2022.102296

Figure Lengend Snippet: Fig. 8. siRNA-mediated knockdown of FoxO1 or FoxO3a increased basal Runx2 levels and H2O2-induced increases in Runx2 in VSMC. A, Western blot showed successful knockdown of FoxO1 by FoxO1-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO1 (indicated by the increase in pFoxO1 (S256)). In these studies, FoxO1 siRNA (P < 0.05), but not H2O2 (P = 0.07), affected levels of pFoxO1(S253) in VSMC, but there was an interaction effect (P < 0.05) between H2O2 and FoxO1 siRNA on pFoxO1(S256). (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Knockdown of FoxO1 increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO1 siRNA (P < 0.05) affected levels of Runx2 in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO1-specific siRNA on levels of pFoxO1(S256) was observed. (n = 4 samples in each group; *P < 0.05; Two-way ANOVA) C, Western blot showed successful knockdown of FoxO3a by FoxO3a-specific siRNA. Addition of H2O2 into the medium induced the inhibition of FoxO3a (indicated by the increase in pFoxO3a(S253)). In these studies, H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of pFoxO3a(S253) in VSMC and an interaction effect (P < 0.05) between H2O2 and FoxO3a siRNA on pFoxO3a(S253) was observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA). D, Knockdown of FoxO3a increased basal expression of Runx2 and increased H2O2-mediated increases in Runx2. H2O2 (P < 0.05) and FoxO3a siRNA (P < 0.05) affected levels of Runx2 in VSMC, but an interaction effect (P > 0.05) between H2O2 and FoxO3a siRNA on levels of pFoxO3a(S253) was not observed (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA).

Article Snippet: RNA interference was accomplished using Catalase Human siRNA Oligo Duplex (Locus ID 847, OriGene Technologies Inc., Rockvile, MD), SignalSilence® FoxO1 siRNA I (Cat# 6242, Cell Signaling Technology, Danvers, MA), and SignalSilence® FoxO3a siRNA I (Cat# 6302, Cell Signaling Technology, Danvers, MA); Trilencer-27 universal scrambled negative siRNA (Cat# SR30004, OriGene Technologies Inc.) served as a control in these experiments.

Techniques: Knockdown, Western Blot, Inhibition, Expressing

Fig. 9. Effect of siRNA-mediated knockdown of catalase on catalase protein, activity, and bioavailable H2O2. in VSMC. A, Western blotting demonstrated successful knockdown of catalase protein by catalase-specific siRNA. Addition of H2O2 into the culture medium decreased (P < 0.05) levels of catalase in VSMC that received nontargeting siRNA. H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affected catalase protein levels in VSMC and an interaction effect (P < 0.05) between H2O2 and catalase siRNA on catalase was observed. (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Catalase activity followed a similar pattern, with H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affecting catalase activity in VSMC and an interaction effect (P < 0.05) between H2O2 and catalase siRNA on catalase was observed. (n = 8 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) C, H2O2 accumulation during vehicle treatment of VSMC pre-treated with siRNA that targeted catalase and VSMC that received nontargeting siRNA did not differ (*P > 0.05). In contrast, incubation of VSMC that received nontargeting siRNA in medium containing H2O2, 0.4 mM, showed significant (**P < 0.05) increases in H2O2 at each time point, compared with the vehicle-treated groups. Incubation in medium containing H2O2, 0.4 mM, of VSMC pre-treated with siRNA that targeted catalase produced significantly higher (***P < 0.05) amounts of H2O2 at each time point, compared with the other three groups in the study. (n = 8 samples in each group; one-way ANOVA).

Journal: Redox biology

Article Title: Dietary salt initiates redox signaling between endothelium and vascular smooth muscle through NADPH oxidase 4.

doi: 10.1016/j.redox.2022.102296

Figure Lengend Snippet: Fig. 9. Effect of siRNA-mediated knockdown of catalase on catalase protein, activity, and bioavailable H2O2. in VSMC. A, Western blotting demonstrated successful knockdown of catalase protein by catalase-specific siRNA. Addition of H2O2 into the culture medium decreased (P < 0.05) levels of catalase in VSMC that received nontargeting siRNA. H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affected catalase protein levels in VSMC and an interaction effect (P < 0.05) between H2O2 and catalase siRNA on catalase was observed. (n = 4 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) B, Catalase activity followed a similar pattern, with H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affecting catalase activity in VSMC and an interaction effect (P < 0.05) between H2O2 and catalase siRNA on catalase was observed. (n = 8 samples in each group; ns, not significant; *P < 0.05; Two-way ANOVA) C, H2O2 accumulation during vehicle treatment of VSMC pre-treated with siRNA that targeted catalase and VSMC that received nontargeting siRNA did not differ (*P > 0.05). In contrast, incubation of VSMC that received nontargeting siRNA in medium containing H2O2, 0.4 mM, showed significant (**P < 0.05) increases in H2O2 at each time point, compared with the vehicle-treated groups. Incubation in medium containing H2O2, 0.4 mM, of VSMC pre-treated with siRNA that targeted catalase produced significantly higher (***P < 0.05) amounts of H2O2 at each time point, compared with the other three groups in the study. (n = 8 samples in each group; one-way ANOVA).

Article Snippet: RNA interference was accomplished using Catalase Human siRNA Oligo Duplex (Locus ID 847, OriGene Technologies Inc., Rockvile, MD), SignalSilence® FoxO1 siRNA I (Cat# 6242, Cell Signaling Technology, Danvers, MA), and SignalSilence® FoxO3a siRNA I (Cat# 6302, Cell Signaling Technology, Danvers, MA); Trilencer-27 universal scrambled negative siRNA (Cat# SR30004, OriGene Technologies Inc.) served as a control in these experiments.

Techniques: Knockdown, Activity Assay, Western Blot, Incubation, Produced

Fig. 10. Effect of siRNA-mediated knockdown of catalase on H2O2-induced decreases in PP2a activity and increases in Runx2 in VSMC. A and B, Immu noprecipitation of the active subunit of PP2a and subsequent analysis of PP2a activity in the immunoprecipitants showed that loss of catalase enhanced the effects of H2O2 on PP2a activity, but neither catalase knockdown nor H2O2 altered the amount of PP2a C subunit. In these experiments, H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affected PP2a activity in VSMC but there was no interaction (P > 0.05) between H2O2 and catalase siRNA on PP2a activity. (n = 8 samples in each group; *P < 0.05; Two-way ANOVA) C, Knockdown of catalase enhanced the effects of H2O2 on Runx2 in VSMC. H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affected Runx2 levels in VSMC and an interaction effect (P < 0.05) between H2O2 and catalase siRNA on Runx2 was observed. (n = 4 samples in each group; *P < 0.05; Two- way ANOVA).

Journal: Redox biology

Article Title: Dietary salt initiates redox signaling between endothelium and vascular smooth muscle through NADPH oxidase 4.

doi: 10.1016/j.redox.2022.102296

Figure Lengend Snippet: Fig. 10. Effect of siRNA-mediated knockdown of catalase on H2O2-induced decreases in PP2a activity and increases in Runx2 in VSMC. A and B, Immu noprecipitation of the active subunit of PP2a and subsequent analysis of PP2a activity in the immunoprecipitants showed that loss of catalase enhanced the effects of H2O2 on PP2a activity, but neither catalase knockdown nor H2O2 altered the amount of PP2a C subunit. In these experiments, H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affected PP2a activity in VSMC but there was no interaction (P > 0.05) between H2O2 and catalase siRNA on PP2a activity. (n = 8 samples in each group; *P < 0.05; Two-way ANOVA) C, Knockdown of catalase enhanced the effects of H2O2 on Runx2 in VSMC. H2O2 (P < 0.05) and catalase siRNA (P < 0.05) affected Runx2 levels in VSMC and an interaction effect (P < 0.05) between H2O2 and catalase siRNA on Runx2 was observed. (n = 4 samples in each group; *P < 0.05; Two- way ANOVA).

Article Snippet: RNA interference was accomplished using Catalase Human siRNA Oligo Duplex (Locus ID 847, OriGene Technologies Inc., Rockvile, MD), SignalSilence® FoxO1 siRNA I (Cat# 6242, Cell Signaling Technology, Danvers, MA), and SignalSilence® FoxO3a siRNA I (Cat# 6302, Cell Signaling Technology, Danvers, MA); Trilencer-27 universal scrambled negative siRNA (Cat# SR30004, OriGene Technologies Inc.) served as a control in these experiments.

Techniques: Knockdown, Activity Assay