Journal: medRxiv

Article Title: JYNNEOS vaccination induced short-lived neutralizing antibody responses to monkeypox virus in naïve individuals

doi: 10.1101/2024.08.28.24312561

Figure Lengend Snippet: Serum specimens from eight JYNNEOS vaccine recipients were analyzed for IgG reactivity to recombinant protein antigens derived from mpox or vaccinia virus by microsphere immunoassay. One donor with who received ACAM2000 prior to JYNNEOS is shown separately in A and B. Means of seven individuals who had no prior smallpox vaccination are shown in C and D. Mean index values (MFI/cutoff) of MPXV E8L (gray squares), MPXV A35R (blue triangles) and MPXV H3L (white diamonds) were plotted for days 0, 8, 26, 56, 118, 231, and 434 post-vaccination (A,C). Mean index values (MFI/cutoff) of VACV L1R (white circles), VACV A33R (orange triangles) and VACV B5R (gray circles) were plotted for 0, 8, 26, 56, 118, 231, and 434 post-vaccination (B, D). The black dashed line at y = 1.0 indicates the cutoff value. The dotted line indicates the second dose of vaccine at day 28 post vaccination.

Article Snippet: Recombinant A33R (VAC-WR-A33R; Cat# NR545), B5R (VAC-WR-B5R; Cat# NR-546) and L1R (VAC-WR-L2R; Cat# NR-21986) were obtained from BEI Resources, Manassas VA. Mpox A35R (Cat # 230-30238), E8L (Cat# 230-30232) and H3L (Cat# 230-30233) were purchased from Ray Biotech, Peachtree Corners, GA.

Techniques: Recombinant, Derivative Assay, Virus

Journal: bioRxiv

Article Title: A novel assay to isolate and quantify third-stage filarial larvae emerging from individual mosquitoes

doi: 10.1101/2020.02.04.934653

Figure Lengend Snippet: ( A ) Infection and assay protocol followed in this study. Mosquitoes are fed on blood containing D. immitis microfilariae. Uptake is measured in a small group, and the remaining mosquitoes are maintained until an emergence assay is performed. Immediately following the emergence assay with whole mosquitoes, the Malpighian tubules are dissected and analyzed. The dissected head and carcass are individually placed into an emergence assay to assay L3 that failed to emerge from intact mosquitoes. shows images of dissected mosquitoes and Malpighian tubules. ( B ) Mosquitoes are rinsed with 70% ethanol to wet, rinsed with water, and ( C ) placed individually into wells of a 96-well plate. ( D ) Emerging third-stage larvae (eL3) from intact mosquitoes or L3 from dissected heads and carcasses at the bottom each well are scored by microscopy. Supplemental movie 1 shows typical movement of eL3. ( E ) Larvae (white arrows) in live Malpighian tubules are scored by microscopy. Scale bar is 50 μm.

Article Snippet: Blood containing B. malayi microfilariae ( B. malayi microfilariae in cat blood, live, FR3, NR-48887) was obtained from an experimentally infected cat containing approximately 12,000 mf/mL provided by the NIH/NIAID Filariasis Research Reagent Resource Center for distribution by BEI Resources, NIAID, NIH.

Techniques: Infection, Microscopy

Journal: bioRxiv

Article Title: A novel assay to isolate and quantify third-stage filarial larvae emerging from individual mosquitoes

doi: 10.1101/2020.02.04.934653

Figure Lengend Snippet: ( A ) Dots indicate the number of microfilariae present in the midgut of individual Ae. aegypti R immediately after feeding on infected blood (Uptake) and the number of eL3 assayed 14 days post infection. The black line indicates the median. ( B ) Dots indicate the number of microfilariae present in the midgut of individual Ae. aegypti S immediately after feeding on infected blood (Uptake), the number of eL3 assayed 14 days post infection, the number of L3 emerging from the dissected heads (Head) or carcass (Carcass), and the number present in the Malpighian tubules (MT). The Total is sum of all parasites of any stage found in any tissue or assay on day 14. The red line indicates the median. ( C ) Dots are the number of eL3 from individual Ae. aegypti S and all third-stage larvae assayed (Total; sum of eL3, Head, and Carcass) (data taken from panel B). The red line is the median number. The asterisks indicate a Mann-Whitney P-value < 0.001. These data are the sum of three independent biological replicates. The number of mosquitoes assayed is shown below each column.

Article Snippet: Blood containing B. malayi microfilariae ( B. malayi microfilariae in cat blood, live, FR3, NR-48887) was obtained from an experimentally infected cat containing approximately 12,000 mf/mL provided by the NIH/NIAID Filariasis Research Reagent Resource Center for distribution by BEI Resources, NIAID, NIH.

Techniques: Infection, MANN-WHITNEY

Journal: bioRxiv

Article Title: A novel assay to isolate and quantify third-stage filarial larvae emerging from individual mosquitoes

doi: 10.1101/2020.02.04.934653

Figure Lengend Snippet: ( A ) Dots indicate the number of D. immitis microfilariae present in midguts of Ae. aegypti S (blue) and Ae. aegypti R (red) immediately following blood feeding on the indicated doses of microfilariae. Data in each column are normally distributed and black lines and numbers indicate the mean. No significant differences were found when Ae. aegypti S and Ae. aegypti R were compared at the different doses using an unpaired t test. Data are from two independent biological replicates. ( B ) Graph of the average prevalence of D. immitis eL3 in mosquitoes feeding on blood with increasing concentrations of microfilariae, assayed 17 days post-infection. Error bars indicate the standard deviation. There was no significant difference comparing the columns using and ANOVA. ( C ) Dots are the number of D. immitis eL3 from individual mosquitoes assayed 17 days post-infection. No significant differences were found when we compared all groups to each other or comparing 4000 mf/mL to the other groups using a Kruskal-Wallis test with Dunn’s correction for multiple comparisons. Data for panels B and C are pooled from four separate biological replicates. ( D ) Kaplan Meier survival plot for Ae. aegypti S or ( E ) Ae. aegypti R fed with uninfected blood (BF) or blood containing different concentrations of microfilariae. Pairs of adjacent treatment groups were analyzed by Kaplan Meier, and relationships with significant differences indicated with asterisks in and in .

Article Snippet: Blood containing B. malayi microfilariae ( B. malayi microfilariae in cat blood, live, FR3, NR-48887) was obtained from an experimentally infected cat containing approximately 12,000 mf/mL provided by the NIH/NIAID Filariasis Research Reagent Resource Center for distribution by BEI Resources, NIAID, NIH.

Techniques: Infection, Standard Deviation

Journal: bioRxiv

Article Title: A novel assay to isolate and quantify third-stage filarial larvae emerging from individual mosquitoes

doi: 10.1101/2020.02.04.934653

Figure Lengend Snippet: ( A ) Dots indicate the number of B. malayi microfilariae present in midguts of Ae. aegypti S (blue) and Ae. aegypti R (red) immediately following blood feeding. Data in each column are not normally distributed and black line is the median. The number of mosquitoes (n) analyzed indicated for each sample. No significant difference was using a Mann Whitney test (P=0.09). ( B ) Graph of the average prevalence of B. malayi eL3 assayed 12-14 days post-infection in Ae. aegypti S (blue bars) and at days 12 and 14 in Ae. aegypti R (red bars). Error bars indicate the standard deviation. The number of mosquitoes (n) analyzed indicated for each sample. No emerging parasites were observed for Ae. aegypti R . There were no significant differences between the days assayed for Ae. aegypti S , and all Ae. aegypti S days assayed were significantly different from both Ae. aegypti R on the two days assayed using an ANOVA with Tukey’s multiple comparisons test as indicated by asterisks (P<0.001). ( C ) Blue dots are the number of B. malayi eL3 from individual mosquitoes assayed 12-14 days post-infection. No significant differences were found when we compared all groups to each other or comparing day 12 to the other groups using a Kruskal-Wallis test with Dunn’s correction for multiple comparisons. Data were pooled from three independent experiments.

Article Snippet: Blood containing B. malayi microfilariae ( B. malayi microfilariae in cat blood, live, FR3, NR-48887) was obtained from an experimentally infected cat containing approximately 12,000 mf/mL provided by the NIH/NIAID Filariasis Research Reagent Resource Center for distribution by BEI Resources, NIAID, NIH.

Techniques: MANN-WHITNEY, Infection, Standard Deviation

Journal: MethodsX

Article Title: Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

doi: 10.1016/j.mex.2023.102061

Figure Lengend Snippet:

Article Snippet: Nicotinamide riboside chloride (NR; Cat. No. A220128) was purchased from Cosmo Bio Co., Ltd. (Tokyo, Japan).

Techniques: Modification, Mass Spectrometry, Chromatography