Journal: Journal of Clinical Medicine

Article Title: Validating the Transformation of PROMIS-GH to EQ-5D in Adult Spine Patients

doi: 10.3390/jcm8101506

Figure Lengend Snippet: Mean (standard deviation (SD)) of actual and estimated EQ-5D Index Scores, R 2 values, correlation coefficients, mean squared errors (MSE), and mean absolute errors (MAE) for models in the spine patient sample ( N = 121).

Article Snippet: To be more conservative, sample sizes of at least 30 patients are suggested for the good mean estimate of a EQ-5D score from PROMIS-GHS using the CAT Reg model.

Techniques: Standard Deviation

Journal: Journal of Clinical Medicine

Article Title: Validating the Transformation of PROMIS-GH to EQ-5D in Adult Spine Patients

doi: 10.3390/jcm8101506

Figure Lengend Snippet: Comparison of actual EQ-5D scores and estimates of the CAT reg model by subgroups.

Article Snippet: To be more conservative, sample sizes of at least 30 patients are suggested for the good mean estimate of a EQ-5D score from PROMIS-GHS using the CAT Reg model.

Techniques: Comparison, Biomarker Discovery

Journal: Journal of Clinical Medicine

Article Title: Validating the Transformation of PROMIS-GH to EQ-5D in Adult Spine Patients

doi: 10.3390/jcm8101506

Figure Lengend Snippet: Bland-Altman agreement plot. X axis is the average score of the actual and estimated EQ-5D score of the CAT reg model. Y axis is the difference between the two. Each dot represents a patient. The three dashed lines are upper 95% limits of agreements (mean + 1.96 SD), mean difference, and lower 95% limits of agreements (mean − 1.96 SD).

Article Snippet: To be more conservative, sample sizes of at least 30 patients are suggested for the good mean estimate of a EQ-5D score from PROMIS-GHS using the CAT Reg model.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Validating the Transformation of PROMIS-GH to EQ-5D in Adult Spine Patients

doi: 10.3390/jcm8101506

Figure Lengend Snippet: Histograms of observed EQ-5D index scores and estimates from CAT Reg , CAT LE , and CAT equip.

Article Snippet: To be more conservative, sample sizes of at least 30 patients are suggested for the good mean estimate of a EQ-5D score from PROMIS-GHS using the CAT Reg model.

Techniques:

Journal: The EMBO Journal

Article Title: TNFSF14 + natural killer cells prevent spontaneous abortion by restricting leucine-mediated decidual stromal cell senescence

doi: 10.1038/s44318-024-00220-3

Figure Lengend Snippet: ( A – C ) C57BL/6 pregnant mice were fed with control (1.2% leucine) ( n = 3 biological replicates) or high leucine (6%) ( n = 4 biological replicates) fodder, the SAβG activity of DSCs were measured by flow cytometry, and expression of CDKN2A, CDKN1A, and TP53 of DSCs were detected by immunofluorescence. ( D ) The embryo resorption rate, the weight of placenta and embryo of pregnant mice were quantified at the gestation of day 13.5 (control fodder: n = 3 biological replicates, high leucine fodder: n = 4 biological replicates). ( E ) Decidualized hESCs were co-cultured with dNK cells for another 48 h, and CDKN2A, CDKN1A, and TP53 expression were detected by western blotting ( n = 3 biological replicates per group), relative expression levels of proteins were standardized using internal reference GAPDH. ( F ) Compared the expression of TNFSF14 on dNK cells of NP ( n = 6 biological replicates) and SA ( n = 6 biological replicates) by flow cytometry. ( G ) Compared the expression of TNFRSF14 on DSCs of NP ( n = 6 biological replicates) and SA ( n = 6 biological replicates) by Immunohistochemistry. Scale bar, 100 μm. ( H , I ) Decidualized si TNFRSF14 -hESCs were co-cultured with dNK cells for another 48 h, western blotting indicated CDKN2A, CDKN1A, and TP53 expression ( n = 3 biological replicates per group), relative expression levels of proteins were standardized using internal reference GAPDH, and immunofluorescence showed SAβG + cells ( n = 4 biological replicates per group). NP: normal pregnancy; RSA: recurrent spontaneous abortion. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, ns, no significance, using a one-tailed ( A , B , D ), two-tailed, unpaired Student’s t test ( E , F , H , I ). .

Article Snippet: Tnfrsf14 knockout mice (cat. NO. NM-KO-190170) were constructed by Shanghai Model Organisms Center, Inc. (Shanghai, China).

Techniques: Control, Activity Assay, Flow Cytometry, Expressing, Immunofluorescence, Cell Culture, Western Blot, Immunohistochemistry, One-tailed Test, Two Tailed Test

Journal: The EMBO Journal

Article Title: TNFSF14 + natural killer cells prevent spontaneous abortion by restricting leucine-mediated decidual stromal cell senescence

doi: 10.1038/s44318-024-00220-3

Figure Lengend Snippet: ( A , B ) At the gestation of day 13.5, embryo resorption, weight of placenta and embryo in Tnfrsf14 knockout pregnant mice ( Tnfrsf14 −/− ♀×WT♂, n = 5 biological replicates) and WT pregnant mice (WT♀× Tnfrsf14 −/− ♂, n = 5 biological replicates) were recorded. ( C , D ) The SAβG activity of DSCs were measured by flow cytometry, and expression of CDKN2A, CDKN1A and TP53 of DSCs were detected by immunofluorescence ( n = 5 biological replicates per group). ( E ) Schematic diagram of decidualized hESCs treated with recombinant TNFSF14 protein. ( F , G ) After decidualized hESCs were treated with TNFSF14 (250 ng/mL, 48 h), SAβG + cells were detected by immunofluorescence, and CDKN2A, CDKN1A, and TP53 expression were measured by western blotting ( n = 3 biological replicates per group), relative expression levels of proteins were standardized using internal reference GAPDH. Scale bar, 100 μm. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, ns, no significance, using a one-tailed ( B – D ), two-tailed, unpaired Student’s t test ( F , G ). .

Article Snippet: Tnfrsf14 knockout mice (cat. NO. NM-KO-190170) were constructed by Shanghai Model Organisms Center, Inc. (Shanghai, China).

Techniques: Knock-Out, Activity Assay, Flow Cytometry, Expressing, Immunofluorescence, Recombinant, Western Blot, One-tailed Test, Two Tailed Test

Journal: The EMBO Journal

Article Title: TNFSF14 + natural killer cells prevent spontaneous abortion by restricting leucine-mediated decidual stromal cell senescence

doi: 10.1038/s44318-024-00220-3

Figure Lengend Snippet: ( A ) Bubble diagram showed the enrichment of pathway for DSCs treated with TNFSF14. ( B ) Heat map showed the relative expression of differential gene for DSCs treated with TNFSF14. ( C , D ) Decidualized hESCs were treated with TNFSF14 (250 ng/mL), or co-cultured with dNK cells (5 × 10 5 ) for 48 h, SLC3A2 expression levels were detected with western blotting ( n = 3 biological replicates per group), relative expression levels of proteins were standardized using internal reference Tubulin. ( E , F ) Decidualized si TNFRSF14 hESCs were co-cultured with dNK cells for 48 h, SLC3A2 expression were detected by western blotting ( n = 3 biological replicates per group), relative expression levels of proteins were standardized using internal reference Tubulin. ( G ) Decidualized hESCs were treated with TNFSF14 (250 ng/mL), or decidualized si TNFRSF14 hESCs were co-cultured with dNK cells for 48 h, and then the concentration of BCAA was measured ( n = 6 biological replicates per group). ( H ) After decidualized hESCs were treated with TNFSF14 (250 ng/mL, 48 h), the transcriptional levels of SLC3A2 and HSF1 were detected by RT-PCR ( n = 6 biological replicates per group). ( I ) Dual luciferase reporter assays were conducted in hESCs to verify the combination of HSF1 and WT or mutated SLC3A2 promoter region ( n = 4 biological replicates per group). Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, using a two-tailed, unpaired Student’s t test. .

Article Snippet: Tnfrsf14 knockout mice (cat. NO. NM-KO-190170) were constructed by Shanghai Model Organisms Center, Inc. (Shanghai, China).

Techniques: Expressing, Cell Culture, Western Blot, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Luciferase, Two Tailed Test

Journal: The EMBO Journal

Article Title: TNFSF14 + natural killer cells prevent spontaneous abortion by restricting leucine-mediated decidual stromal cell senescence

doi: 10.1038/s44318-024-00220-3

Figure Lengend Snippet: ( A ) DSCs of spontaneous abortion were treated with metformin (2.5 mM) for 48 h, CDKN2A, CDKN1A, and TP53 expression were detected by western blotting ( n = 3 biological replicates per group), relative expression levels of proteins were standardized using internal reference GAPDH. ( B , C ) After treatment with metformin (200 mg/kg, every other day) in aging pregnant female mice, number of blastocyst implantation, embryo absorption, weight of placenta and embryo were counted at the gestation of day 13.5 (D-galactose group: n = 3 biological replicates, metformin group: n = 3 biological replicates). ( D , E ) Tnfrsf14 -/- pregnant mice were treated with metformin by gavage ( n = 3, biological replicates per group, 200 mg/kg, every other day), embryo resorption, weight of placenta and embryo were calculated at the gestation of day 13.5. ( F , G ) SAβG activity of DSCs were measured by flow cytometry, and CDKN2A, CDKN1A, and TP53 expression of DSCs were verified by immunofluorescence ( n = 3 biological replicates per group). Scale bar, 100 μm. ( H ) Schematic roles of decidual NK cells in preventing spontaneous abortion by maintaining homeostasis of DSC senescence during early pregnancy. During decidualization, increased BCAA transport mediated by SLC3A2 induces senescent DSCs, whereas TNFSF14 + dNK cells control DSC senescence by the TNFSF14/TNFRSF14-SLC3A2/leucine regulatory axis. Excessive senescence of DSC induced by the imbalance of TNFSF14/TNFRSF14-SLC3A2/leucine regulatory axis increases the risk of adverse pregnancy outcomes. The image was drew by the Figdraw. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using a one-tailed ( C , E – G ), two-tailed, unpaired Student’s t test ( A ). .

Article Snippet: Tnfrsf14 knockout mice (cat. NO. NM-KO-190170) were constructed by Shanghai Model Organisms Center, Inc. (Shanghai, China).

Techniques: Expressing, Western Blot, Activity Assay, Flow Cytometry, Immunofluorescence, Control, One-tailed Test, Two Tailed Test