Journal: Journal of Biological Chemistry

Article Title: Probing Conformational Rescue Induced by a Chemical Corrector of F508del-Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Mutant

doi: 10.1074/jbc.m111.239699

Figure Lengend Snippet: FIGURE 5. The effect of chemical correction and ablation of the R553XR555 sequence on conformational correction of F508del-CFTR probed using limited proteolysis. Limited proteolysis of control or chemically corrected F508del-CFTR, F508del-KXK-CFTR, or WT-CFTR with increasing levels of trypsin (0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 g/ml) was performed. Digests were analyzed by SDS-PAGE, and immunoreactive bands were detected using the Odyssey infrared imaging system from LI-COR Biosciences. The colored signal was converted to grayscale for the preparation of this figure. A, samples were immuno- blotted with anti-CFTR monoclonal antibody, L12B4 (NBD1-specific). Subsequent analyses focused on the larger NBD1 fragments (34–36 kDa) and the smaller NBD1 fragments (lower than 28 kDa). Both sets of fragments were indicated using brackets on the right-hand side of each image. B, samples were immuno- blotted with anti-CFTR monoclonal antibody, M3A7 (NBD2-specific). NBD2 fragments of 28 kDa were analyzed subsequently (indicated using brackets on the right-hand side of each image).

Article Snippet: L12B4 anti-CFTR antibody (1:1000) was used for immunodetection conjugated to IRDye800 (1:10 000) (Rockland Immunochemical Inc).

Techniques: Sequencing, Control, SDS Page, Imaging

Journal: STAR Protocols

Article Title: Alginate-based 3D cancer cell culture for therapeutic response modeling

doi: 10.1016/j.xpro.2021.100391

Figure Lengend Snippet:

Article Snippet: T8+ Dispensehead Cassettes , Tecan , 30097370.

Techniques: Recombinant, Saline, Viability Assay, Software, High Content Screening, Sterility, Suction Filtration, Cell Culture, Transferring

Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Article Title: Glial activation in the early stages of brain metastasis: TSPO as a diagnostic biomarker.

doi: 10.2967/jnumed.113.127449

Figure Lengend Snippet: FIGURE 4. (A) Representative SPECT images at injection site (left hemi- sphere) from 4T1-GFP–injected mouse and saline-injected control. (B) 123I- DPA713 binding was confirmed by autoradiography in 4T1-GFP–injected animals as compared with saline-injected animals. (C–D) Graphs show percentage signal increase in injected hemisphere, compared with control contralateral hemisphere for 4T1-GFP– (n 5 6) and saline- (n 5 3) injected animals for either SPECT (C) or autoradiography (D) data. *P , 0.05.

Article Snippet: Screens were held in autoradiography cassettes (Fisher Biotech) for 36 h at 4 C. Subsequently, the phosphor plate was read with Cyclone Plus using OptiQuant software (Perkin Elmer).

Techniques: Single Photon Emission Computed Tomography, Injection, Saline, Control, Binding Assay, Autoradiography

Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Article Title: Glial activation in the early stages of brain metastasis: TSPO as a diagnostic biomarker.

doi: 10.2967/jnumed.113.127449

Figure Lengend Snippet: FIGURE 5. Representative autoradiography image from mouse brain obtained 21 d after in- tracardiac injection of 4T1-GFP cells; areas of increased signal correlated spatially with immu- nohistochemical detection of tumor burden, as seen in immunohistochemistry image. Graph shows quantification of 123I-DPA713 binding (normalized activity), as determined by SPECT, in animals injected intracardially with either 4T1-GFP cells or saline.

Article Snippet: Screens were held in autoradiography cassettes (Fisher Biotech) for 36 h at 4 C. Subsequently, the phosphor plate was read with Cyclone Plus using OptiQuant software (Perkin Elmer).

Techniques: Autoradiography, Injection, Immunohistochemistry, Binding Assay, Activity Assay, Single Photon Emission Computed Tomography, Saline