Journal: International Journal of Molecular Sciences

Article Title: Therapeutic Efficiency of Proteins Secreted by Glial Progenitor Cells in a Rat Model of Traumatic Brain Injury

doi: 10.3390/ijms241512341

Figure Lengend Snippet: Immunoblotting for caspase-9: ( A ) representative blots, ( B ) semi-quantitation data presented as means ± SD; * p < 0.05 ( t -test).

Article Snippet: The membranes were pre-blocked overnight with 3% BSA in PBS and incubated with primary antibodies to p-Tau Ser396 (1:1000, ab32057), p-Tau Thr205 (1:1000, ab254410), APP (1:1000, ab32136) (all by Abcam, UK), Tau (1:1000, 46687) (Cell Signaling, Danvers, MA, USA), and β-actin (1:1000, 8457) (Cell Signaling) for neurodegeneration marker analysis and β-actin (1:2000, A1978) (Sigma-Aldrich) and cleaved-CASP9 (D353) antibody (1:500, CSB-PA000010) (Cusabio, Wuhan, China) for caspase-9 analysis in PBS with 0.1% Tween-20 and 1% BSA.

Techniques: Western Blot, Quantitation Assay

Journal: Scientific Reports

Article Title: Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

doi: 10.1038/s41598-020-69939-3

Figure Lengend Snippet: Genes evaluated by RT-qPCR using Applied BiosystemsTaqMan Assay 83 .

Article Snippet: Bt04282453_m1 , CAT , Catalase.

Techniques: RNA Binding Assay, Histone Deacetylase Assay, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Eugenol-Induced Autophagy and Apoptosis in Breast Cancer Cells via PI3K/AKT/FOXO3a Pathway Inhibition

doi: 10.3390/ijms22179243

Figure Lengend Snippet: The effect of eugenol treatment on caspase-3 and caspase-9 mRNA and protein expression levels. MDA-MB231 and SK-BR-3 cells were treated for 24 h ( A , B ). Thereafter, caspase-3 and caspase-9 mRNA expression levels were quantified by RT-PCR. The protein expression levels of caspase-3 and caspase-9 were then assessed in MDA-MB231 and SK-BR3 cells treated for 24 h with 0, 4, and 8 μM and 0, 5, and 10 μM eugenol, respectively ( C , D ). * indicates a significant change compared to untreated cells (0 μM), # indicates a significant change from MDA-MB-231 at eugenol concentration 4 μM, and $ indicates significant changes from SK-BR-3 treated with 10 μM eugenol at p < 0.05 based on a one-way ANOVA and the Tukey–Kramer post-ANOVA test.

Article Snippet: We performed real-time PCR using gene expression master mix (Applied Biosystems, Foster City, CA, USA) on the ABI PRISM 7500 Detection System (Applied Biosystems, USA) to detect the expression levels of FOXO3 (Hs00818121_m1), P21 (Hs01040810_m1), cyclin-dependent kinase inhibitor 1B (P27Kip1), AKT (Hs00178289_m1), caspase-3 (Hs00234387_m1), caspase-9 (Hs00962278_m1), MAP1LC3A (Hs01076567_g1), nucleoporin 62 (Hs02621445_s1), and BCL2 interacting protein 3 (Hs00969291_m1).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Concentration Assay