cas9 viral particles Search Results


particles expressing cas9  (Beyotime)
99
Beyotime particles expressing cas9
Particles Expressing Cas9, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/Cas9+NLS/pmc10184426-111-11-23
Average 99 stars, based on 1 article reviews
particles expressing cas9 - by Bioz Stars, 2026-10
99/100 stars
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dead cas9 vp64  (Addgene inc)
96
Addgene inc dead cas9 vp64
Dead Cas9 Vp64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/lenti+dCAS-VP64_Blast+(Plasmid+%2361425)/bio_rxiv__2022__04__03__486870-192-19-21
Average 96 stars, based on 1 article reviews
dead cas9 vp64 - by Bioz Stars, 2026-10
96/100 stars
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cas9 viral particles  (Addgene inc)
96
Addgene inc cas9 viral particles
Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and <t>Cas9</t> control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).
Cas9 Viral Particles, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/lentiCas9-Blast+(Plasmid+%2352962)/pmc06617392-590-5-21
Average 96 stars, based on 1 article reviews
cas9 viral particles - by Bioz Stars, 2026-10
96/100 stars
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ihh crispr/cas9 lenti-virus packaging particles >108 tu/ml  (VectorBuilder GmbH)
90
VectorBuilder GmbH ihh crispr/cas9 lenti-virus packaging particles >108 tu/ml
Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and <t>Cas9</t> control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).
Ihh Crispr/Cas9 Lenti Virus Packaging Particles >108 Tu/Ml, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/lentiviral+vectors/pm36552817-71-0-17
Average 90 stars, based on 1 article reviews
ihh crispr/cas9 lenti-virus packaging particles >108 tu/ml - by Bioz Stars, 2026-10
90/100 stars
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simple-western-system-0  (Bio-Techne corporation)
99
Bio-Techne corporation simple-western-system-0
Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and <t>Cas9</t> control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).
Simple Western System 0, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/simple-western-system-0/bio-techne+corporation___simple-western-system
Average 99 stars, based on 1 article reviews
simple-western-system-0 - by Bioz Stars, 2026-10
99/100 stars
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zetasizer advance  (Micromeritics Instrument)
99
Micromeritics Instrument zetasizer advance
Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and <t>Cas9</t> control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).
Zetasizer Advance, supplied by Micromeritics Instrument, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/Zetasizer+Advance/custom%40zetasizer-advance-01%4033647431
Average 99 stars, based on 1 article reviews
zetasizer advance - by Bioz Stars, 2026-10
99/100 stars
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brunello genome wide crispr cas9 library46  (Addgene inc)
96
Addgene inc brunello genome wide crispr cas9 library46
Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and <t>Cas9</t> control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).
Brunello Genome Wide Crispr Cas9 Library46, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/Human+CRISPR+Knockout+Pooled+Library+(Brunello)+(Pooled+Library+%2373179%2C+%2373179-LV%2C+%2373178%2C+%2373178-LV)/pm37044098-258-42-52
Average 96 stars, based on 1 article reviews
brunello genome wide crispr cas9 library46 - by Bioz Stars, 2026-10
96/100 stars
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004-650  (Bio-Techne corporation)
99
Bio-Techne corporation 004-650
Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and <t>Cas9</t> control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).
004 650, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/004-650/bio-techne+corporation___004-650
Average 99 stars, based on 1 article reviews
004-650 - by Bioz Stars, 2026-10
99/100 stars
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Image Search Results


Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and Cas9 control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).

Journal: Cancer Cell

Article Title: Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer

doi: 10.1016/j.ccell.2019.05.013

Figure Lengend Snippet: Functional Impact of RAS/RAF Mutations and NF1 Inactivation on Cetuximab Sensitivity (A) Western blot of BRAF and KRAS mutants in DiFi cells. Quantification of pERK signal relative to total ERK as a loading control, and normalized to luciferase control. (B) Western blot following NF1 (siNF1) or control (siCON) small interfering RNA in LIM1215 cells. Quantification of pERK signal relative to total ERK, and normalized to untreated control. (C) Sanger sequencing of LIM1215 cells transduced with two CRISPR guide RNAs against NF1 . Guide sequences are highlighted by a black bar. (D) Western blot of CRISPR-inactivated NF1 and Cas9 control cells with/without 24 h cetuximab treatment. Quantification of pERK signal relative to total ERK and normalized to untreated Cas9 control. (E) Growth of CRISPR-inactivated NF1 and Cas9 control cells by crystal violet staining (left) and quantification (right).

Article Snippet: LIM1215 cells were transduced with Cas9 viral particles (a gift from Feifei Song, Stephen Pettitt and Chris Lord, derived from lentiCas9-Blast (Addgene # 52962, a gift from Feng Zhang)) in the presence of Polybrene (8 μg/mL) and selected with 5 μg/mL Blasticidin to create constitutively expressing Cas9 lines, confirmed by Western blotting using Cas9 (7A9-3A3) antibody (Cell Signalling Technologies #14697).

Techniques: Functional Assay, Western Blot, Control, Luciferase, Small Interfering RNA, Sequencing, Transduction, CRISPR, Staining

Journal: Cancer Cell

Article Title: Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer

doi: 10.1016/j.ccell.2019.05.013

Figure Lengend Snippet:

Article Snippet: LIM1215 cells were transduced with Cas9 viral particles (a gift from Feifei Song, Stephen Pettitt and Chris Lord, derived from lentiCas9-Blast (Addgene # 52962, a gift from Feng Zhang)) in the presence of Polybrene (8 μg/mL) and selected with 5 μg/mL Blasticidin to create constitutively expressing Cas9 lines, confirmed by Western blotting using Cas9 (7A9-3A3) antibody (Cell Signalling Technologies #14697).

Techniques: Control, Recombinant, Sequencing, Software