Journal: bioRxiv

Article Title: Efficient identification of antimalarials with novel mechanism of action via multi-sample transcriptomic profiling

doi: 10.1101/2025.02.27.640686

Figure Lengend Snippet: a Schematic representation of donor DNA insertion into the HDAC1 locus using CRISPR-Cas9 (left panel). Five silent mutations are inserted into the sgRNA target sequence of both control and L137F mutation donor DNAs (right panel). b Dose-response curves of Novel A (left panel) and Novel A derivative Compound (right panel) for the parental Dd2 line, HDAC1:L137F mutant lines, and HDAC1:silent mutation control lines (four technical replicates and three independent experiments, mean ± SD). c The structure of Compound 1. d PfHDAC1/ Compound 1 complex model for the wildtype (left panel) and L137F mutant (right panel). Compound 1 is depicted in green, and the L137 or F137 amino acid is highlighted with the electron density of atoms. Yellow dot lines represent the hydrogen bonds between the compound and amino acids. The red dot line indicates the collision between F137 and Compound 1. yDHODH: yeast dihydroorotate dehydrogenase, sgRNA: single guide RNA, HR: homologous region, Amp: ampicillin-resistant gene, PAM: protospacer adjacent motif.

Article Snippet: P. falciparum Dd2 strain and pUF-Cas9-pre-sgRNA obtained from Addgene were used for CRISPR-Cas9 genome editing.

Techniques: CRISPR, Sequencing, Control, Mutagenesis

Journal: Cell reports

Article Title: High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation

doi: 10.1016/j.celrep.2020.02.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: pCC_01 - lenti-hU6-sgRNA{F+E)-EFS-Gas9-NLS-2A-Puro , This study , Addgene, plasmid #139086.

Techniques: Virus, Recombinant, DNA Extraction, DNA Purification, Staining, Plasmid Preparation, Software