Journal: Biological & pharmaceutical bulletin

Article Title: Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy.

doi: 10.1248/bpb.b16-00542

Figure Lengend Snippet: Fig. 1. Enzymatic Activity of Cas9 Was Confirmed by in Vitro Plasmid Cleavage Assay

Article Snippet: The membranes were blocked in Blocking One (Nacalai Tesque, Kyoto, Japan) and then incubated with diluted Guide-itTM Cas9 Polyclonal Antibody (TaKaRa Bio USA, Mountain View, CA, U.S.A.).

Techniques: Activity Assay, In Vitro, Plasmid Preparation, Cleavage Assay

Journal: Biological & pharmaceutical bulletin

Article Title: Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy.

doi: 10.1248/bpb.b16-00542

Figure Lengend Snippet: Fig. 2. Cas9 Was Digested in Simulated Gastric Fluid (SGF) for the Indicated Periods (min)

Article Snippet: The membranes were blocked in Blocking One (Nacalai Tesque, Kyoto, Japan) and then incubated with diluted Guide-itTM Cas9 Polyclonal Antibody (TaKaRa Bio USA, Mountain View, CA, U.S.A.).

Techniques:

Journal: Biological & pharmaceutical bulletin

Article Title: Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy.

doi: 10.1248/bpb.b16-00542

Figure Lengend Snippet: Fig. 3. Cas9 Was Digested in Simulated Gastric Fluid for the Indicated Periods (min), Which Were Longer Than Those in Fig. 2

Article Snippet: The membranes were blocked in Blocking One (Nacalai Tesque, Kyoto, Japan) and then incubated with diluted Guide-itTM Cas9 Polyclonal Antibody (TaKaRa Bio USA, Mountain View, CA, U.S.A.).

Techniques:

Journal: Biological & pharmaceutical bulletin

Article Title: Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy.

doi: 10.1248/bpb.b16-00542

Figure Lengend Snippet: Fig. 4. Thermal Stability of Cas9 Was Examined

Article Snippet: The membranes were blocked in Blocking One (Nacalai Tesque, Kyoto, Japan) and then incubated with diluted Guide-itTM Cas9 Polyclonal Antibody (TaKaRa Bio USA, Mountain View, CA, U.S.A.).

Techniques:

Journal: Experimental Biology and Medicine

Article Title: Nuclear factor E2-related factor 2 knockdown enhances glucose uptake and alters glucose metabolism in AML12 hepatocytes

doi: 10.1177/1535370217694435

Figure Lengend Snippet: Antibodies used for Western blot analysis

Article Snippet: The ratios of the mean values of protein level in AML12 cells between two selected groups are listed in Supplementary Table 2. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Company Reference Dilution Nrf2 Santa Cruz Sc-722 1:1000 HO-1 Abcam ab52947 1:1000 NQO1 Proteintech 11451-1-AP 1:1000 p-EIF2α S51 Millipore 04-342 1:1000 EIF2α Proteintech 11233-1-AP 1:1000 IL-1β Ruiying Biological RLT2322 1:1000 TNF-α Ruiying Biological RLM3477 1:1000 p-NF-κB p65 S276 Ruiying Biological RLP0187 1:1000 MMP2 Ruiying Biological RLT2798 1:1000 MMP9 Ruiying Biological RLT1892 1:1000 FGF21 Abcam ab171941 1:1000 AMPKα Ruiying Biological RLT0215 1:1000 Sirt1 Cell signaling Q96E86 1:1000 PGC-1α Abcam ab54481 1:1000 UCP1 Abcam ab23841 1:1000 Glut-4 Ruiying Biological RLT1930 1:1000 IGF-1R Ruiying Biological RLT2282 1:1000 FOXO1 Ruiying Biological RLT1757 1:1000 p-AKT S473 Ruiying Biological RLP0006 1:1000 AKT Ruiying Biological RLT0178 1:1000 GSK3α/β Proteintech 22104-1-AP 1:500 p-GSK3α/β Y279/Y216 Signalway 11002 1:500 Gapdh Santa Cruz Sc420485 1:1000 Open in a separate window Antibodies used for Western blot analysis Statistical analysis The data are presented as the mean ± SEM for the number of replicates indicated.

Techniques: Western Blot