Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: PAR2 (Protease-Activated Receptor 2) Deficiency Attenuates Atherosclerosis in Mice

doi: 10.1161/atvbaha.117.310082

Figure Lengend Snippet: Figure 1. PAR2 (protease-activated receptor 2) mRNA and protein are increased in the atherosclerotic prone regions of mice and humans. Normal mouse aortic arch (low-density lipoprotein receptor–deficient [Ldlr−/−] mice fed a chow diet for 24 wk) or atherosclerosis-containing aortic arches (Ldlr−/− mice fed a Western diet for 24 wk) were examined for (A) mRNA extrapolated to normal and (B) protein expression (n=5 individual samples per group). Normal or diseased human carotid arteries were isolated and examined for (C) mRNA extrapolated to normal and (D) protein expression (n=5 individual samples per group). Immunologic detection of PAR2 expression was also performed on mouse atherosclerotic aortic sinus (E, F) and human carotid atherosclerotic lesions (H, I; n=4–5 individual samples per species; ×4 magnification E and H; ×10 magnification F and I). G, J, Quantification of PAR2 staining in the lesion or media of atherosclerotic lesions. Solid white box is zoomed in area. Dashed white line is the area of the lesion. Histobars represent mean±SEM. *P<0.05 for comparisons to normal aorta/artery or lesion vs tunica media (2-tailed Student t test).

Article Snippet: Immunostaining was performed on frozen serial sections as described previously.26 Human carotid atherosclerotic sections were obtained from Origene Technologies, Inc.

Techniques: Western Blot, Expressing, Isolation, Staining

Journal: Medical Devices (Auckland, N.Z.)

Article Title: Consistency and sealing of advanced bipolar tissue sealers

doi: 10.2147/MDER.S79642

Figure Lengend Snippet: Ex vivo burst pressures for porcine carotid arteries transected with ENSEAL G2 and LigaSure. Notes: Median values (mmHg) and total number of vessels included (n), are shown. + P <0.05 comparing LigaSure with both ENSEAL devices; *outlier data point.

Article Snippet: Fresh Porcine carotid arteries were obtained from Animal Biotech Industries (Danboro, PA, USA).

Techniques: Ex Vivo

Journal: Antioxidants

Article Title: Pulse Wave Velocity Is Associated with Increased Plasma oxLDL in Ageing but Not with FGF21 and Habitual Exercise

doi: 10.3390/antiox9030221

Figure Lengend Snippet: Pearson’s correlation between age with pulse wave velocity (PWV) ( A ), as a surrogate marker for arterial stiffness, and oxidised low-density lipoprotein (oxLDL) concentrations ( B ). One-way analysis of variance was conducted for PWV ( C ) and oxLDL concentrations ( D ) between weekly exercise duration, stratified across four groups. Each symbol represents an individual that participated in 0 min (circle), <180 min (square), 180–360 min (upward triangle) and >360 min (downward triangle) of weekly exercise for the past five years. ns = not significant.

Article Snippet: Arterial stiffness was measured indirectly using PWV between the carotid and femoral arteries (SphygmoCor XCEL, AtCor Medical Pvt, Ltd., NSW, Sydney, Australia).

Techniques: Marker

Journal: Antioxidants

Article Title: Pulse Wave Velocity Is Associated with Increased Plasma oxLDL in Ageing but Not with FGF21 and Habitual Exercise

doi: 10.3390/antiox9030221

Figure Lengend Snippet: Two-way analysis of variance (age × exercise) of fibroblast growth factor 21 (FGF21) ( A ), adiponectin ( B ), oxidized low-density lipoprotein (oxLDL) ( C ) concentrations and pulse wave velocity (PWV) ( D ) in younger active (YA) ( n = 20, M/F = 10/10) and sedentary (YS) ( n = 20, M/F = 10/10), and in older active (OA) ( n = 20, M/F = 11/9) and sedentary (OS) ( n = 20, M/F = 12/8) individuals. Two-way ANOVA for FGF21 concentration was conducted only for YA ( n = 19, M/F = 10/9), YS ( n = 19, M/F = 9/10), OA ( n = 20, M/F = 11/9) and OS ( n = 20, M/F = 12/8) groups *** = p < 0.001 for age. † = p < 0.05 for exercise status. Black circles represent active individuals and white circles represent sedentary individuals within younger and older groups.

Article Snippet: Arterial stiffness was measured indirectly using PWV between the carotid and femoral arteries (SphygmoCor XCEL, AtCor Medical Pvt, Ltd., NSW, Sydney, Australia).

Techniques: Concentration Assay

Journal: Antioxidants

Article Title: Pulse Wave Velocity Is Associated with Increased Plasma oxLDL in Ageing but Not with FGF21 and Habitual Exercise

doi: 10.3390/antiox9030221

Figure Lengend Snippet: Pearson’s correlations ( r ) between FGF21, adiponectin, oxLDL and pulse wave velocity (PWV).

Article Snippet: Arterial stiffness was measured indirectly using PWV between the carotid and femoral arteries (SphygmoCor XCEL, AtCor Medical Pvt, Ltd., NSW, Sydney, Australia).

Techniques: Biomarker Discovery