Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma.

doi: 10.1002/advs.202416809

Figure Lengend Snippet: Figure 4. CARM1 is highly expressed in ccRCC and promotes proliferation. A,B) CARM1 is highly expressed in ccRCC patients and is correlated with poor prognosis on the UALCAN database. C) CARM1 and H3R17me2a are highly expressed in ccRCC cells such as 786-O, A498, and Caki-1. D,E) Immunofluorescence staining using the tissue microarray revealed that CARM1 is highly expressed in ccRCC tissue samples. Scale bar = 100 μm. (Tumor n = 79, Normal n = 79) F) CcRCC patients with advanced T stage have higher CARM1 expression, which may be associated with poor prognosis.

Article Snippet: The antibodies used in this study are as follows: LEDGF (Abcam, ab177159), CARM1 (CST, #3379), H3R17me2a (Acive motif, #39 710), PRMT6 (Abcam, ab271091), H3K36me3 (CST, #4909), PPAT (Proteintech, #15401- 1-AP), PAICS (Proteintech, #12967-1-AP), GART (Proteintech, #13659-1- AP), ADSL (Proteintech, #15264-1-AP), ADSS2 (Proteintech, #16373-1- AP), Histone H3 (Proteintech, #17168-1-AP), Alpha Actin (Proteintech, #23660-1-AP), Flag (Abmart, #M20008), Goat Anti-Rabbit IgG (H + L) (Proteintech,#SA00001-2), and Goat Anti-Mouse IgG (H + L) (Proteintech, #SA00001-1).

Techniques: Staining, Microarray, Expressing

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma.

doi: 10.1002/advs.202416809

Figure Lengend Snippet: Figure 5. Construction of the H3R17me2a-deficient cell lines. A,B) Validation of knockout efficiency of CARM1 and the reduction of H3R17me2a level in transduced pooled A498 cells (A) and 786-O cells (B). C–E) Validation of knockout efficiency of CARM1 and the reduction of H3R17me2a level in monoclonal A498 cells (C) and 786-O cells (D-E). F,G) Knockdown of PRMT6 using siRNA has little effect on H3R17me2a level in A498 cells (F) and 786-O cells (G). H) The combined knockdown of CARM1 and PRMT6 using siRNAs reduces H3R17me2a level in A498 cells; knockdown of PRMT6 in CARM1-KO cells significantly decreased H3R17me2a in A498 cells. I) Knockdown of PRMT6 in CARM1-KO cells significantly decreased H3R17me2a in A498 cells. J) The combination of CARM1 (MCE, EZM2302 and TP-064) and PRMT6 (MCE, EPZ020411) inhibitors can significantly reduce H3R17me2a level in A498 cells.

Article Snippet: The antibodies used in this study are as follows: LEDGF (Abcam, ab177159), CARM1 (CST, #3379), H3R17me2a (Acive motif, #39 710), PRMT6 (Abcam, ab271091), H3K36me3 (CST, #4909), PPAT (Proteintech, #15401- 1-AP), PAICS (Proteintech, #12967-1-AP), GART (Proteintech, #13659-1- AP), ADSL (Proteintech, #15264-1-AP), ADSS2 (Proteintech, #16373-1- AP), Histone H3 (Proteintech, #17168-1-AP), Alpha Actin (Proteintech, #23660-1-AP), Flag (Abmart, #M20008), Goat Anti-Rabbit IgG (H + L) (Proteintech,#SA00001-2), and Goat Anti-Mouse IgG (H + L) (Proteintech, #SA00001-1).

Techniques: Biomarker Discovery, Knock-Out, Knockdown

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma.

doi: 10.1002/advs.202416809

Figure Lengend Snippet: Figure 8. Deficiency of LEDGF protects NKG mice against xenograft proliferation. A) Schematic diagram of subcutaneous tumor model in NKG mice in indicated treatment groups. All surviving mice were euthanized 8 weeks after tumor cell inoculation. B) Knock out of LEDGF effectively reduced the proliferation of xenografts in NKG mice. (n = 5) C) There was no significant difference in body weight between the two groups throughout the experiment. D–F) Elimination of LEDGF effectively reduced the volume (D-E) and weight (F) of NKG mice xenografts. G) QRT-PCR was used to demonstrate that decrease of LEDGF can reduce mRNA expression of PPAT, PAICS, GART, ADSL, and ADSS2 in xenograft tumors. H) The proliferation ability of xenografts in LEDGF-KO group was significantly reduced. The expression levels of PPAT, PAICS, GART, and ADSL were significantly decreased, while ADSS2 expres- sion was almost unchanged. Scale bar = 100 μm. I) A schematic model illustrating that LEDGF interacts with CARM1-mediated H3R17me2a to promote ccRCC progression. Data are shown as mean ± SD. ***p < 0.001. ns means no significance.

Article Snippet: The antibodies used in this study are as follows: LEDGF (Abcam, ab177159), CARM1 (CST, #3379), H3R17me2a (Acive motif, #39 710), PRMT6 (Abcam, ab271091), H3K36me3 (CST, #4909), PPAT (Proteintech, #15401- 1-AP), PAICS (Proteintech, #12967-1-AP), GART (Proteintech, #13659-1- AP), ADSL (Proteintech, #15264-1-AP), ADSS2 (Proteintech, #16373-1- AP), Histone H3 (Proteintech, #17168-1-AP), Alpha Actin (Proteintech, #23660-1-AP), Flag (Abmart, #M20008), Goat Anti-Rabbit IgG (H + L) (Proteintech,#SA00001-2), and Goat Anti-Mouse IgG (H + L) (Proteintech, #SA00001-1).

Techniques: Knock-Out, Quantitative RT-PCR, Expressing

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A Western blotting analysis showed the expression of CARM1 in 35 pairs of HCC and adjacent normal liver tissues. B The expression of CARM1 in 66 pairs of HCC and corresponding adjacent normal liver tissues was examined via an IHC assay. C Kaplan-Meier survival analysis of overall survival in HCC patients stratified by CARM1 expression. Patients with low expression ( n = 19) had lower expression values in HCC tissues than in normal tissues, while patients with high expression ( n = 47) had higher expression values in HCC tissues than in normal tissues. D CARM1 mRNA levels in normal and primary HCC tumor tissues from the TCGA database. E Kaplan-Meier survival analysis of HCC patients from the TCGA database was performed according to CARM1 mRNA expression. Patients with high CARM1 expression had expression values in the >3rd quantile, while patients with low CARM1 expression had expression values in the <3rd quartile.

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Western Blot, Expressing

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A Representative confocal microscopy image of IF colocalization analysis of CARM1 and PSMD14 in HCC cell lines. Scale bar, 20 μm. B Endogenous PSMD14 proteins were immunoprecipitated with anti-PSMD14 antibodies and then analyzed by immunoblotting (left panel). Endogenous CARM1 proteins were immunoprecipitated with anti-CARM1 antibodies and then analyzed by immunoblotting (right panel). The IgG antibody was used as the control. C Western blotting assays showed the expression of CARM1 in control and PSMD14-knockdown HCC cells. D CARM1 mRNA expression levels in control and PSMD14-knockdown HCC cells were determined by qRT-PCR. E The protein expression levels of CARM1 were assessed by Western blotting analysis in HCC cells with either empty vector or PSMD14 overexpression. F The mRNA expression levels of CARM1 were assessed by qRT-PCR in HCC cells with either control or PSMD14 overexpression. G Control and PSMD14-knockdown HCC cells were treated with CHX (20 µM) for the indicated times, and endogenous CARM1 protein expression was detected by Western blotting analysis (left). The expression levels of CARM1 were determined by densitometry. The level of CARM1 protein in CHX-untreated cells (0 h) was set to 100% ( n = 3; * p < 0.05 and ** p < 0.01).

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Confocal Microscopy, Immunoprecipitation, Western Blot, Control, Expressing, Knockdown, Quantitative RT-PCR, Plasmid Preparation, Over Expression

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A Control and PSMD14-knockdown Huh7 cells were treated with MG132 at 10 μM for 6 h. The cell lysates were immunoprecipitated with an anti-CARM1 antibody, and the immunocomplexes were immunoblotted with anti-CARM1 and anti-ubiquitin antibodies. B Huh7 cells were pretreated with 10 μM capzimin for 24 h, and cells treated with equal amounts of DMSO served as controls. All cells were treated with MG132 at 10 μM for 6 h. The cell lysates were then immunoprecipitated with anti-CARM1 antibodies, and the immunocomplexes were immunoblotted with anti-CARM1 and anti-ubiquitin antibodies. C FLAG-CARM1 and HA-Ubi were transiently transferred into HEK293T cells expressing GFP-PSMD14-WT or GFP-PSMD14-MUT. Cells were then treated with MG132 (10 μM) for 6 h. The cell lysates were immunoprecipitated with an anti-FLAG M2 affinity gel. The ubiquitination levels of CARM1 were detected using anti-HA antibodies. D FLAG-CARM1 and HA-Ubi were transiently transferred into HEK293T cells, which were subsequently purified with FLAG antibodies and protein G beads. Moreover, GFP-PSMD14-WT and GFP-PSMD14-MUT were separately transferred into another two sets of HEK293T cells and purified with GFP antibodies and protein G beads. The purified Ubi-FLAG-CARM1 and GFP-PSMD14 proteins were incubated for 1 h. Then, FLAG-CARM1 was immunoprecipitated with anti-FLAG M2 affinity beads and detected with the indicated antibodies. E HEK293T cells were transfected with the indicated plasmids and treated with MG132 (10 μM) for 6 h. The cell lysates were immunoprecipitated with anti-FLAG M2 affinity beads. The ubiquitination levels of CARM1 were detected using anti-HA antibodies. F Schematic illustration of CARM1 truncation plasmids and lysine sites predicted to be modified by ubiquitination. G HEK293T cells expressing GFP-PSMD14 were transfected with FLAG-tagged full-length or truncated CARM1. The cell lysates were immunoprecipitated with anti-GFP antibodies. H HEK293T cells were transfected with the indicated plasmids and treated with MG132 (10 μM) for 6 h. The cell lysates were immunoprecipitated with an anti-FLAG M2 affinity gel. The ubiquitination levels of CARM1 were detected using anti-HA antibodies.

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Control, Knockdown, Immunoprecipitation, Expressing, Purification, Incubation, Transfection, Modification

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A CARM1 was transfected into HCC cells with PSMD14 knockdown. A CCK-8 assay was performed to detect proliferation. B CARM1 was transfected into HCC cells with PSMD14 knockdown. Then, a colony formation assay was conducted. C CARM1 was transfected into HCC cells with PSMD14 knockdown. Then, a transwell assay was performed to detect migration and invasion. Representative images of the transwell assay are shown. The cells in five randomly selected fields were counted under a microscope. D Representative images of immunohistochemical staining of PSMD14 and CARM1 in the same HCC and corresponding adjacent normal liver tissues are shown. E Correlation analysis of PSMD14 and CARM1 in HCC tissues. The data were statistically analyzed by the Chi-square test. R indicates the Pearson correlation coefficient. F Scatter diagram showing a positive correlation between PSMD14 and CARM1 in HCC tissues by IHC. G Survival analysis of HCC patients was conducted using Kaplan-Meier plots and log-rank tests. The patients were categorized into high and low PSMD14 and CARM1 expression groups based on IHC staining. ( n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Transfection, Knockdown, CCK-8 Assay, Colony Assay, Transwell Assay, Migration, Microscopy, Immunohistochemical staining, Staining, Expressing, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A Western blotting analysis showed the knockdown efficacy of CARM1 in Huh7 and PLC/PRF/5 cells infected with lentiviral particles expressing shRNAs targeting CARM1. B Proliferation of control and CARM1-knockdown Huh7 cells was detected by CCK-8 assays on the indicated days. C Colony formation assays were performed to detect the proliferation of control and CARM1-knockdown HCC cells. The data are presented in a bar chart. D Control or CARM1-knockdown Huh7 cells were subcutaneously injected into nude mice for observation of tumor growth. E The tumor volume was measured every three days and is presented as a line graph. F The tumor weights of the xenografts from the different groups were calculated. G Immunohistochemical analysis of mouse subcutaneous tumors was performed with anti-CARM1 and anti-Ki-67 antibodies. ( n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Western Blot, Knockdown, Infection, Expressing, Control, CCK-8 Assay, Injection, Immunohistochemical staining

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A Representative images of the transwell assay results for control and CARM1-knockdown Huh7 and PLC/PRF/5 cells showing their migration and invasion ability (left). The cells in five randomly selected fields were counted under a microscope, and the data were presented as a bar chart (right). B Representative microscopy images of pulmonary metastatic lesions 8 weeks after the injection of the indicated Huh7 cells into the tail vein of nude mice. C The number of lung metastatic tumors in each group was determined. ( n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Transwell Assay, Control, Knockdown, Migration, Microscopy, Injection

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: A RNA-seq analysis revealed genes whose expression was upregulated (red) or downregulated (blue) in control and CARM1-knockdown Huh7 cells. B Schematic illustration of the peaks identified by ChIP-seq analysis with an anti-CARM1 antibody in Huh7 cells. C Integration of ChIP-seq and RNA-seq data. The Venn diagram shows the overlap between targets and differentially expressed genes. D Detection of the mRNA levels of CARM1 and FERMT1 in control and CARM1-knockdown Huh7 cells by qRT-PCR. E Schematic representation of the four segments near the TSS of FERMT1. ChIP primers were designed for each of the four sequences. F A ChIP assay was performed to detect CARM1 enrichment in the FERMT1 promoter region using an anti-CARM1 antibody. G Western blotting analysis was performed to show the expression level of H3R17me2 in control and CARM1-knockdown cells. H ChIP assay was performed to detect H3R17me2 enrichment in the FERMT1 promoter region using an anti-H3R17me2 antibody. The IgG antibody was used as the negative control. The inhibitory effect of CARM1 depletion on Huh7 cells, as demonstrated by rescue experiments, was effectively counteracted by the overexpression of FERMT1, as shown by both the CCK-8 ( I ) and transwell ( J ) assays. (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: RNA Sequencing Assay, Expressing, Control, Knockdown, ChIP-sequencing, Quantitative RT-PCR, Western Blot, Negative Control, Over Expression, CCK-8 Assay

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: PSMD14-mediated deubiquitination upregulates CARM1 expression, which in turn transcriptionally activates its downstream target FERMT1 through histone H3R17me2. This PSMD14-CARM1-FERMT1 signaling axis significantly promotes HCC growth and metastasis. Pharmacological inhibition of CARM1 using SGC2085 effectively suppresses the malignant phenotypes of HCC cells, suggesting a potential therapeutic strategy for HCC treatment.

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Expressing, Inhibition

Journal: Cell Death & Disease

Article Title: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

doi: 10.1038/s41419-025-07416-3

Figure Lengend Snippet: Correlations between CARM1 expression and the clinicopathological features of patients with HCC.

Article Snippet: Serial sections were deparaffinized, hydrated, and incubated in 3% H 2 O 2 for 20 min at room temperature and then stained with anti-PSMD14 (1:3200, A9608, ABclonal) and anti-CARM1 (1:1000, NB200-342, Novus) at 4 °C overnight.

Techniques: Expressing

Journal: The Journal of Biological Chemistry

Article Title: Inhibition of coactivator-associated arginine methyltransferase 1 modulates dendritic arborization and spine maturation of cultured hippocampal neurons

doi: 10.1074/jbc.M117.775619

Figure Lengend Snippet: Reduction of CARM1 expression increases the complexity of dendritic arborization of cultured hippocampal neurons. A, cultured hippocampal neurons were untreated or transduced with control scrambled (Cont), CARM1-specific, or PRMT1-specific siRNA at 9 DIV for 5 days. At 14 DIV, neurons were lysed and used for total RNA isolation and RT-qPCR. Relative CARM1 mRNA level was analyzed after normalization with GAPDH mRNA level. B, immunoblotting results with anti-CARM1, anti-PRMT1, and anti-actin antibodies from hippocampal neurons treated as in (A). C, cultured hippocampal neurons treated as in (A) were fixed at 14 DIV and stained with MAP-2, a dendritic marker. Whole cell shape is shown. Scale bar, 30 μm. D and E, quantitative analysis of TDBL (μm) and TDBTN from tracing images of neurons treated as in (C). F and G, Sholl analysis of dendritic complexity in neurons treated as in (C). Mean ± S.E., n = 40 neurons/condition from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001, compared with untreated condition.

Article Snippet: After a 2-day incubation, neurons were untreated or transduced with rat CARM1- or PRMT1-specific siRNA or scrambled control siRNA lentiviral particles (5 × 10 3 viral particles/μl) (Santa Cruz Biotechnology) at a multiplicity of infection (m.o.i.) of 4 to ensure efficient infection following the manufacturer's protocol, followed by overnight incubation.

Techniques: Expressing, Cell Culture, Transduction, Control, Isolation, Quantitative RT-PCR, Western Blot, Staining, Marker

Journal: The Journal of Biological Chemistry

Article Title: Inhibition of coactivator-associated arginine methyltransferase 1 modulates dendritic arborization and spine maturation of cultured hippocampal neurons

doi: 10.1074/jbc.M117.775619

Figure Lengend Snippet: Reduction of CARM1 expression modifies dendritic spine maturation in cultured hippocampal neurons. A, cultured hippocampal neurons were untreated or transfected with GFP-actin at 7 DIV. After 2 days, cells were transduced with control siRNA (Cont) or CARM1-specific siRNA and incubated for another 5 days. At 14 DIV, neurons were fixed and dendritic spine morphology was visualized by confocal microscopy. Scale bar, 10 μm. B–D, spine length (B), spine head width (C), or spine density (D) (number of spines/10-μm dendrite length) was calculated and statistically analyzed. E, dendritic spine shape was counted and expressed as %. Mean ± S.E., n > 100 spines, from three independent experiments. *, p < 0.05, compared with untreated condition).

Article Snippet: After a 2-day incubation, neurons were untreated or transduced with rat CARM1- or PRMT1-specific siRNA or scrambled control siRNA lentiviral particles (5 × 10 3 viral particles/μl) (Santa Cruz Biotechnology) at a multiplicity of infection (m.o.i.) of 4 to ensure efficient infection following the manufacturer's protocol, followed by overnight incubation.

Techniques: Expressing, Cell Culture, Transfection, Transduction, Control, Incubation, Confocal Microscopy

Journal: The Journal of Biological Chemistry

Article Title: Inhibition of coactivator-associated arginine methyltransferase 1 modulates dendritic arborization and spine maturation of cultured hippocampal neurons

doi: 10.1074/jbc.M117.775619

Figure Lengend Snippet: Knockdown of CARM1 protein promotes post-synaptic clustering of NR2B and PSD-95 proteins. A–C, cultured hippocampal neurons were untreated or transduced with control (Cont) or CARM1-specific siRNA at 9 DIV and incubated for 5 days. At 14 DIV, neurons were fixed and used for double-staining with CARM1 and synapsin (A), NR2B (B), or PSD-95 (C) antibodies. Scale bar, 10 μm. D and E, Synaptic cluster size (μm2) and synaptic clustering (number per 10 μm) were calculated and statistically analyzed. Mean ± S.E., three independent experiments. *, p < 0.05, **, p < 0.01, compared with untreated).

Article Snippet: After a 2-day incubation, neurons were untreated or transduced with rat CARM1- or PRMT1-specific siRNA or scrambled control siRNA lentiviral particles (5 × 10 3 viral particles/μl) (Santa Cruz Biotechnology) at a multiplicity of infection (m.o.i.) of 4 to ensure efficient infection following the manufacturer's protocol, followed by overnight incubation.

Techniques: Knockdown, Cell Culture, Transduction, Control, Incubation, Double Staining

Journal: Medicine

Article Title: The protein arginine methyltransferases (PRMTs) PRMT1 and CARM1 as candidate epigenetic drivers in prostate cancer progression

doi: 10.1097/md.0000000000027094

Figure Lengend Snippet: Figure 1. Graphical representation of the nuclear and cytoplasmic expression of PRMT1 and CARM1 across the spectrum of PCa progression. PCa=prostate cancer, PRMTs=protein arginine methyltransferases.

Article Snippet: Antigen Dilution Source AR 1:50 Dako, Carpentaria, CA, USA CARM1 1:750 Novus Biologicalis.

Techniques: Expressing

Journal: Medicine

Article Title: The protein arginine methyltransferases (PRMTs) PRMT1 and CARM1 as candidate epigenetic drivers in prostate cancer progression

doi: 10.1097/md.0000000000027094

Figure Lengend Snippet: Figure 3. CARM1 expression increases from non-neoplastic to neoplastic cells and from low grade, to high grade to treated cases (original magnification 400). PCa=prostate cancer, PRMTs=protein arginine methyltransferases.

Article Snippet: Antigen Dilution Source AR 1:50 Dako, Carpentaria, CA, USA CARM1 1:750 Novus Biologicalis.

Techniques: Expressing

Journal: Medicine

Article Title: The protein arginine methyltransferases (PRMTs) PRMT1 and CARM1 as candidate epigenetic drivers in prostate cancer progression

doi: 10.1097/md.0000000000027094

Figure Lengend Snippet: Figure 4. PRMT1, CARM1, ZEB1, and TWIST1 expression correlated with one-another in PCa (original magnification 400). A case with low expression of all markers is shown in the upper panel and a case with high expression of all markers in the lower panel. PCa=prostate cancer, PRMTs=protein arginine methyltransferases.

Article Snippet: Antigen Dilution Source AR 1:50 Dako, Carpentaria, CA, USA CARM1 1:750 Novus Biologicalis.

Techniques: Expressing