Journal: Cancer science

Article Title: Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate.

doi: 10.1111/j.1349-7006.2008.00816.x

Figure Lengend Snippet: Fig. 1. Fhl1 and Sdpr expression in normal epi- thelium and tumors from human breast, kidney, and prostate. Immunohistochemical staining was performed on normal and tumor human tissue. Fhl1 and Sdpr staining was evident in normal epithelial cells of the breast, kidney, and prostate. In contrast, both Fhl1 and Sdpr expression were suppressed in infiltrating mammary duct carcinoma, renal cell carcinoma, and prostate adenocarcinoma. (bar = 60 microns).

Article Snippet: Slides containing 15 infiltrating duct carcinoma specimens with normal adjacent tissue from breast cancer patients (IMH-371, Imgenex), nine prostate adenocarcinoma specimens with adjacent normal tissue and an additional unmatched 41 prostate adenocarcinoma specimens (IMH-303, Imgenex), and nine renal cell carcinoma specimens with adjacent normal tissue and an additional unmatched 31 cell carcinoma specimens (IMH-313, Imgenex) were examined by immunohistochemistry as previously described. (19) Briefly, after removal of paraffin with xylene and rehydration through a graded series of decreasing concentrations of ethanol, protein antigenicity was enhanced by microwaving sections in Target Buffer (Dako, Carpenturia, CA, USA) for 2 min.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Biomolecules

Article Title: Structure and Dynamics of Human Chemokine CCL16—Implications for Biological Activity

doi: 10.3390/biom12111588

Figure Lengend Snippet: CCL16 expression in the liver occurs homeostatically. CCL16 mRNA was quantified in normal human liver tissue ( n = 4) in comparison to livers with cirrhosis ( n = 7), hepatitis B virus (HBV) and hepatitis C virus (HCV) infection ( n = 4–5), as well as cholangiocarcinoma (CCC; n = 7) and hepatocellular carcinoma (HCC; n = 7). Values are expressed as relative units (RU) normalised to 18S. Measurements of individual samples and the mean of each group are shown. A Mann–Whitney U test was performed to assess statistical significance (* p < 0.05).

Article Snippet: Normal human liver RNA samples ( n = 4) were commercially available (Clontech; AMS Biotechnology, Abingdon, United Kingdom); diseased liver tissues with liver cirrhosis, hepatitis B virus (HBV) infection with and without tumour, hepatitis C virus (HCV) infection with and without tumour, cholangiocarcinoma (CCC), and hepatocellular carcinoma (HCC) were obtained from the Universitätstumorzentrum (Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany).

Techniques: Expressing, Infection, MANN-WHITNEY

Journal: The American Journal of Pathology

Article Title: Genome-Wide Somatic Copy Number Alterations and Mutations in High-Grade Pancreatic Intraepithelial Neoplasia

doi: 10.1016/j.ajpath.2018.03.012

Figure Lengend Snippet: Clinicopathologic Features of Included Patients

Article Snippet: Patients and Tissue Samples Pancreatic tissues were obtained from patients undergoing pancreatic resection at the Johns Hopkins Hospital from 2009 to 2014.

Techniques: Biomarker Discovery

Journal: The American Journal of Pathology

Article Title: Genome-Wide Somatic Copy Number Alterations and Mutations in High-Grade Pancreatic Intraepithelial Neoplasia

doi: 10.1016/j.ajpath.2018.03.012

Figure Lengend Snippet: A: Scatter plot shows correlation between log R ratio (LRR) of CDKN2A copy number alterations (CNAs) from single nucleotide polymorphism array and copy number ratio of CDKN2A/RNaseP from droplet digital PCR analysis. Spearman's correlation test was used to compare the correlation. R2 means coefficient of determination. B: Comparison of CDKN2A/RNaseP ratio between high-grade pancreatic intraepithelial neoplasia (HG-PanIN) samples with or without copy number loss. Horizontal bar represents the median value. Nonparametric U-test was used to compare ratio variables. ∗∗P < 0.01. CNV, copy number variation.

Article Snippet: Patients and Tissue Samples Pancreatic tissues were obtained from patients undergoing pancreatic resection at the Johns Hopkins Hospital from 2009 to 2014.

Techniques: Digital PCR, Comparison

Journal: The American Journal of Pathology

Article Title: Genome-Wide Somatic Copy Number Alterations and Mutations in High-Grade Pancreatic Intraepithelial Neoplasia

doi: 10.1016/j.ajpath.2018.03.012

Figure Lengend Snippet: Chromothripsis and copy number aberrations plot from CTLPScanner. Highlighted chromothripsis-like regions are shown in the left side of the graph. The x and y axes represent genomic positions and log R ratio of segments, respectively. The orange lines represent areas of copy number gain; blue lines, areas of copy number loss; zero lines, no gain/loss; orange rectangles, identified chromothripsis regions. Histograms on the right side show the number of copy number segment change events. Chr, chromosome; HG-PanIN, high-grade pancreatic intraepithelial neoplasia; LG-PanIN, low-grade pancreatic intraepithelial neoplasia.

Article Snippet: Patients and Tissue Samples Pancreatic tissues were obtained from patients undergoing pancreatic resection at the Johns Hopkins Hospital from 2009 to 2014.

Techniques:

Journal: British Journal of Cancer

Article Title: EGFR/Ras-induced CCL20 production modulates the tumour microenvironment

doi: 10.1038/s41416-020-0943-2

Figure Lengend Snippet: a Relative Ras activity in the immortalised keratinocyte cell line HaCaT and in H-RasV12-transfected HaCaT clones was assayed by the EZ-Detect Ras activation kit. b CCL20 protein production- conditioned medium from HaCaT cells and H-RasV12-transfected HaCaT clones, as detected by a CCL20-specific ELISA. c Activated primary keratinocytes were treated with the selective irreversible inhibitor of EGFR erlotinib, and expression of CCL20 was analysed by qPCR. Tumour cells derived from breast cancer, malignant melanoma and head and neck squamous cell carcinoma (HNSCC) overexpress CCL20. d – f Quantitative real-time PCR analysis of CCL20 in cultured normal primary mammary epithelial cells ( n = 2) and breast cancer cell lines ( n = 7) ( d ), cultured normal primary melanocytes ( n = 2) and melanoma cell lines ( n = 13) ( e ), cultured primary mucosal keratinocytes (KC, n = 2), cell lines derived from primary tumours ( n = 10) or metastases ( n = 4) of HNSCC ( f ). g CCL20 expression of tumour tissues derived from breast cancer (primary breast cancer, n = 12, fc = 1.096; breast cancer metastasis, n = 10, fc = 6.048), malignant melanoma (primary melanoma, n = 28, fc = 4.580; subcutaneous metastasis, n = 11, fc = 6.023; distant metastasis, n = 4, fc = 6.012) and HNSCC (primary tumour, n = 14, fc = 3.057) compared with normal tissue (normal breast, n = 3, cultured primary melanocytes, n = 3, benign nevi, n = 5 and normal oral mucosa, n = 8) by qPCR. Values are either expressed as femtograms of target gene per 25 ng of cDNA or protein concentration in picograms per ml of supernatant and represent the mean ± SD of three independent experiments (* P ≤ 0.05; ** P ≤ 0.01; Mann–Whitney U test).

Article Snippet: Tumour tissue microarrays of breast cancer and head and neck squamous cell carcinoma were acquired from Pantomics, Inc. (San Francisco, CA, USA), Super Bio Chips (Seoul, Korea) and US Biomax, Inc. (Rockville, MD, USA).

Techniques: Activity Assay, Transfection, Clone Assay, Activation Assay, Enzyme-linked Immunosorbent Assay, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Cell Culture, Protein Concentration, MANN-WHITNEY