Journal: PLoS ONE

Article Title: Development of a constant pressure perfused ex vivo model of the equine larynx

doi: 10.1371/journal.pone.0251530

Figure Lengend Snippet: A: The perfusion system (technical drawing) consists of a closed circuit. Red color indicates the hot water circuit, blue represents the perfusion circuit, gassed with carbogen. For active perfusion, the arteries were catheterized, passive venous outflow drains into the perfusion tub. The gassing tubes (one in the supply tank 1 and one in the perfusion tub) are denoted with an asterisk. The perfusion flow was measured using a flowmeter (F). In the inset photograph, a dorsal view of the perfusion setup in situ can be observed. The larynx, immersed in perfusion solution, is placed on a larynx retainer to stabilize its position in the perfusion tub. Both cranial thyroid arteries were catheterized (intravenous catheter, 18 gauge). For pH-control, a pH-meter is added (marked with an arrow). B: Schematic drawing of an equine larynx, lateral aspect (redrawn after our specimens and corrosions casts of the Institute of Veterinary Anatomy, Leipzig University). Relevant structures are the cricoarytenoideus dorsalis muscle [CAD, a)], the common carotid artery [b) running parallel to the external jugular vein], the catheterized arterial branch [c), cranial thyroid artery] followed by its ramification into the ascending pharyngeal artery (d) and the laryngeal branch (e). The latter two vessels nourish the CAD. f) Recurrent laryngeal nerve reaches the larynx as caudal laryngeal nerve.

Article Snippet: The perfusion solution was permanently gassed with Carbogen (95% O 2 , 5% CO 2 , Linde AG, Pullach, Germany).

Techniques: In Situ, Control

Journal: NPJ Vaccines

Article Title: Calcifediol boosts efficacy of ChAdOx1 nCoV-19 vaccine by upregulating genes promoting memory T cell responses

doi: 10.1038/s41541-024-00909-w

Figure Lengend Snippet: Clinical and Biochemical characteristics of the study subjects at all time points

Article Snippet: Of the 24 subjects who underwent screening, 12 received 50 µg calcifediol (2 capsules of 25 µg)/day (Dishman Carbogen Amcis Ltd, Ahmedabad, India) (Calcifediol-supplemented group), and the other 12 received placebo (2 capsules/day) (Placebo group).

Techniques:

Journal: NPJ Vaccines

Article Title: Calcifediol boosts efficacy of ChAdOx1 nCoV-19 vaccine by upregulating genes promoting memory T cell responses

doi: 10.1038/s41541-024-00909-w

Figure Lengend Snippet: Total plasma 25(OH)D, plasma iPTH, serum calcium and plasma calcitriol levels at baseline, 3rd month, and 6th month in both treated ( N = 12) and placebo groups ( N = 12). Here, “pΔ” represents p value of the significant delta changes between placebo and calcifediol supplemented groups. Δ t-baseline, represents change in values of 3rd and 6th month with respect to baseline. Two-way ANOVA was performed to determine the significance. The data is represented as Mean ± SD.

Article Snippet: Of the 24 subjects who underwent screening, 12 received 50 µg calcifediol (2 capsules of 25 µg)/day (Dishman Carbogen Amcis Ltd, Ahmedabad, India) (Calcifediol-supplemented group), and the other 12 received placebo (2 capsules/day) (Placebo group).

Techniques:

Journal: NPJ Vaccines

Article Title: Calcifediol boosts efficacy of ChAdOx1 nCoV-19 vaccine by upregulating genes promoting memory T cell responses

doi: 10.1038/s41541-024-00909-w

Figure Lengend Snippet: a Normalization of raw counts by DESeq 2 using Variance Stabilizing Transformation (VST). b Principal Component Analysis (PCA) of calcifediol supplemented (treated) cohorts, where blue bubbles represent baseline, red bubbles represent 3rd month and green bubbles represent 6th month. c Comparisons of the numbers of differentially expressed genes [differentially expressed (DE) features] at various time points. d Venn Diagrams depicting common and unique Upregulated and ( e ) downregulated differentially expressed genes in the calcifediol supplemented subjects at the 3rd and 6th month.

Article Snippet: Of the 24 subjects who underwent screening, 12 received 50 µg calcifediol (2 capsules of 25 µg)/day (Dishman Carbogen Amcis Ltd, Ahmedabad, India) (Calcifediol-supplemented group), and the other 12 received placebo (2 capsules/day) (Placebo group).

Techniques: Transformation Assay

Journal: NPJ Vaccines

Article Title: Calcifediol boosts efficacy of ChAdOx1 nCoV-19 vaccine by upregulating genes promoting memory T cell responses

doi: 10.1038/s41541-024-00909-w

Figure Lengend Snippet: List of main biological processes and the genes involved that were found differentially upregulated upon calcifediol supplementation

Article Snippet: Of the 24 subjects who underwent screening, 12 received 50 µg calcifediol (2 capsules of 25 µg)/day (Dishman Carbogen Amcis Ltd, Ahmedabad, India) (Calcifediol-supplemented group), and the other 12 received placebo (2 capsules/day) (Placebo group).

Techniques: Activation Assay, Chemotaxis Assay, Virus

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Antimicrobial Activity of Omadacycline Tested against Clinical Bacterial Isolates from Hospitals in Mainland China, Hong Kong, and Taiwan: Results from the SENTRY Antimicrobial Surveillance Program (2013 to 2016)

doi: 10.1128/AAC.02262-18

Figure Lengend Snippet: Antimicrobial activity of omadacycline tested against the main organisms and organism groups of isolates from all countries combined

Article Snippet: Omadacycline powder was purchased from Carbogen AMCIS (Bubendorf, Switzerland), and comparator agents include tigecycline, doxycycline, tetracycline, minocycline, azithromycin, clindamycin, levofloxacin, linezolid, trimethoprim-sulfamethoxazole, ampicillin, amoxicillin-clavulanic acid, cefepime, ceftazidime, ceftriaxone, imipenem, penicillin, amikacin, gentamicin (powders purchased from US Pharmacopeia, Rockville, MD, USA), colistin, oxacillin, vancomycin (Sigma-Aldrich, St. Louis, MO, USA), piperacillin-tazobactam (Sigma-Aldrich and US Pharmacopeia, respectively), and daptomycin (TOKU-E, Bellingham, WA, USA).

Techniques: Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Antimicrobial Activity of Omadacycline Tested against Clinical Bacterial Isolates from Hospitals in Mainland China, Hong Kong, and Taiwan: Results from the SENTRY Antimicrobial Surveillance Program (2013 to 2016)

doi: 10.1128/AAC.02262-18

Figure Lengend Snippet: Summary of omadacycline activity stratified by geographic region a

Article Snippet: Omadacycline powder was purchased from Carbogen AMCIS (Bubendorf, Switzerland), and comparator agents include tigecycline, doxycycline, tetracycline, minocycline, azithromycin, clindamycin, levofloxacin, linezolid, trimethoprim-sulfamethoxazole, ampicillin, amoxicillin-clavulanic acid, cefepime, ceftazidime, ceftriaxone, imipenem, penicillin, amikacin, gentamicin (powders purchased from US Pharmacopeia, Rockville, MD, USA), colistin, oxacillin, vancomycin (Sigma-Aldrich, St. Louis, MO, USA), piperacillin-tazobactam (Sigma-Aldrich and US Pharmacopeia, respectively), and daptomycin (TOKU-E, Bellingham, WA, USA).

Techniques: Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Antimicrobial Activity of Omadacycline Tested against Clinical Bacterial Isolates from Hospitals in Mainland China, Hong Kong, and Taiwan: Results from the SENTRY Antimicrobial Surveillance Program (2013 to 2016)

doi: 10.1128/AAC.02262-18

Figure Lengend Snippet: Activity of omadacycline and comparator antimicrobial agents for the main organisms from all geographic regions combined

Article Snippet: Omadacycline powder was purchased from Carbogen AMCIS (Bubendorf, Switzerland), and comparator agents include tigecycline, doxycycline, tetracycline, minocycline, azithromycin, clindamycin, levofloxacin, linezolid, trimethoprim-sulfamethoxazole, ampicillin, amoxicillin-clavulanic acid, cefepime, ceftazidime, ceftriaxone, imipenem, penicillin, amikacin, gentamicin (powders purchased from US Pharmacopeia, Rockville, MD, USA), colistin, oxacillin, vancomycin (Sigma-Aldrich, St. Louis, MO, USA), piperacillin-tazobactam (Sigma-Aldrich and US Pharmacopeia, respectively), and daptomycin (TOKU-E, Bellingham, WA, USA).

Techniques: Activity Assay