Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

Article Title: Moderate alcohol intake promotes pancreatic ductal adenocarcinoma development in mice expressing oncogenic Kras

doi: 10.1152/ajpgi.00218.2019

Figure Lengend Snippet: Immunofluorescence staining of pancreatic cancer tissues. Pancreas tissues collected from Pdx1Cre;LSL-KrasG12D mice treated with all insults for 5 mo were stained with different antibodies. A: expression of prominin-1 (PROM1) and E-cadherin (CDH1) in pancreatic intraepithelial neoplasia (PanIN)-1a (double arrowheads). An arrowhead indicates acinar cells undergoing acinar-ductal metaplasia (ADM). *Normal acinar cells. B: double arrowheads indicate expression of doublecortin-like kinase 1 (DCLK1) in epithelial cell adhesion molecule positive (EPCAM+) PanIN-1a. C: heterogeneous expression of DCLK1 in PROM1+ tumor cells (arrowheads). D and E: expression of coxsackie virus and adenovirus receptor (CXADR), mesothelin (MSLN), and WW domain containing transcription regulator 1 (WWTR1) in tumors. F: heterogeneous expression of S100 calcium-binding protein A6 (S100A6; arrows) and VCAM1 (arrowheads) in tumors. G: strong expression of vimentin (VIM) in tumor-associated stromal cells. H: expression of α-smooth muscle actin (ACTA2; arrows) in some desmin-positive (DES+) stromal cells around tumors. I: podoplanin (PDPN) expression in the lymphatic vessels (arrow) and mesothelial cells (double arrowheads) in the normal pancreas (wild type). J: strong expression of PDPN in the tumor-associated stroma. K: expression of decorin (DCN) in the stroma. L: expression of nephronectin (NPNT) at the basal side of PanIN (arrowheads). Arrows indicate acinar cells that connect to early PanIN. *Normal acinar cells. M: downregulation of NPNT in EPCAM+ tumor cells that protrude into the stroma (double arrowheads). Nuclei were counterstained with DAPI. Bar = 20 μm.

Article Snippet: Primary antibodies used are: E-cadherin (CDH1) conjugated with AlexaFluor 488 (100-fold dilution, 560061; BD Biosciences, San Jose, CA), doublecortin-like kinase 1 (DCLK1; 100-fold dilution, PA5-20908; Thermo Fisher Scientific, Waltham, MA), prominin-1 (PROM1; 50-fold dilution, 14-1331), S100 calcium-binding protein A6 (S100A6; 100-fold dilution, AF2377), vascular cell adhesion molecule 1 (VCAM1; 50-fold dilution, 14-1061), coxsackie virus and adenovirus receptor (CXADR; 100-fold dilution, AF2654; R&D Systems, Minneapolis, MN), nephronectin (NPNT; 100-fold dilution, AF4298), EPCAM (100-fold dilution, G88; Developmental Studies Hybridoma Bank, Iowa City, IA), KRT19 (50-fold dilution, TROMA-III), mesothelin (MSLN; 100-fold dilution, HPA017172; Sigma), and WWTR1 (50-fold dilution, HPA007415).

Techniques: Immunofluorescence, Staining, Expressing, Virus, Binding Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Significantly Improved Pharmacokinetics Enhances In Vivo Efficacy of APX001 against Echinocandin- and Multidrug-Resistant Candida Isolates in a Mouse Model of Invasive Candidiasis

doi: 10.1128/AAC.00425-18

Figure Lengend Snippet: Pharmacokinetic parameters for APX001A in various mouse strains following a single i.v. or i.p. dosing of 26 mg/kg APX001 alone or after oral pretreatment with 100 mg/kg ABT

Article Snippet: Four-week-old male humanized PXR-CAR-CYP3A4/3A7 mice (Taconic, Rensselaer, NY), male humanized PXR-CAR-CYP3A4/3A7 mice (Taconic), and 6-week-old female CD-1 mice were used in the PK studies.

Techniques: