Journal: Microbiology Spectrum

Article Title: Coxsackievirus A10 blocks autophagosome-lysosome fusion to promote viral nonlytic spread and inflammatory cytokine release

doi: 10.1128/spectrum.00830-25

Figure Lengend Snippet: Inflammatory cytokines and viruses were detected in the extracellular vesicles of CV-A10-infected cells. ( A ) A heatmap presenting the results of inflammatory cytokines in extracellular vesicles by flow cytometry. ( B ) The proliferative dynamics of CV-A10 in extracellular vesicles were assessed by monitoring the viral load, virus titer, and VP1 expression via qRT-PCR, TCID 50 , and WB assays, respectively.

Article Snippet: In this study, we used the commercial Capture Extracellular Vesicle Isolation Kit (Takara, Japan), following the manufacturer’s instructions to obtain extracellular vesicles.

Techniques: Infection, Flow Cytometry, Virus, Expressing, Quantitative RT-PCR

Journal: Microbiology Spectrum

Article Title: Coxsackievirus A10 blocks autophagosome-lysosome fusion to promote viral nonlytic spread and inflammatory cytokine release

doi: 10.1128/spectrum.00830-25

Figure Lengend Snippet: The autophagic secretory pathway was involved in the release of inflammatory cytokines and viruses during CV-A10 infection. ( A ) Protein expression levels of the autophagic secretory pathway were measured by WB in CV-A10-infected HUVECs subjected to different treatments. ( B ) A heatmap presenting the results of inflammatory cytokines in extracellular vesicles through flow cytometry in CV-A10-infected HUVECs subjected to different treatments. ( C ) Examination of extracellular viral RNA, virus titer, and VP1 protein from CV-A10-infected HUVECs subjected to different treatments by qRT-PCR, TCID 50 , and WB assays, respectively.

Article Snippet: In this study, we used the commercial Capture Extracellular Vesicle Isolation Kit (Takara, Japan), following the manufacturer’s instructions to obtain extracellular vesicles.

Techniques: Infection, Expressing, Flow Cytometry, Virus, Quantitative RT-PCR