Journal: Virus research

Article Title: The current state of therapeutic and T cell-based vaccines against human papillomaviruses

doi: 10.1016/j.virusres.2016.12.002

Figure Lengend Snippet: Recent Therapeutic HPV Vaccine Clinical Trials Using Different Forms of Vaccines

Article Snippet: In addition, lists several ongoing clinical trials evaluating the efficacy of therapeutic and T cell based HPV vaccines against HPV-associated diseases. table ft1 table-wrap mode="anchored" t5 caption a7 Vaccine Antigen(s) Construct Organization Trial Design Outcome Side Effects Reference Bacterial Vector Based Lm-LLo-E7 (ADXS 11-001; ADXS-HPV) HPV-16 E7 prfA-defective Listeria monocytogenes strain transformed with plasmid encoding HPV-16 E7 antigen fused to a fragment of nonhemolytic listeriolysin O (LLO) Advaxis, Inc.

Techniques: Construct, Plasmid Preparation, Transformation Assay, Recombinant, Expressing, Modification, Generated, Injection, Infection

Journal: Virus research

Article Title: The current state of therapeutic and T cell-based vaccines against human papillomaviruses

doi: 10.1016/j.virusres.2016.12.002

Figure Lengend Snippet: Currently Ongoing Therapeutic and T cell-based HPV Vaccine Clinical Trials

Article Snippet: In addition, lists several ongoing clinical trials evaluating the efficacy of therapeutic and T cell based HPV vaccines against HPV-associated diseases. table ft1 table-wrap mode="anchored" t5 caption a7 Vaccine Antigen(s) Construct Organization Trial Design Outcome Side Effects Reference Bacterial Vector Based Lm-LLo-E7 (ADXS 11-001; ADXS-HPV) HPV-16 E7 prfA-defective Listeria monocytogenes strain transformed with plasmid encoding HPV-16 E7 antigen fused to a fragment of nonhemolytic listeriolysin O (LLO) Advaxis, Inc.

Techniques: Construct, Infection, Plasmid Preparation, Sequencing, Transformation Assay, Expressing

Journal: Human Gene Therapy Methods

Article Title: Adeno-Associated Virus 5 Transduces Adipose-Derived Stem Cells with Greater Efficacy Than Other Adeno-Associated Viral Serotypes

doi: 10.1089/hgtb.2016.123

Figure Lengend Snippet: Lectins used to determine the glycoconjugate profile of adipose-derived stem cells and the corresponding rAAV serotypes that bind to the glycosylation motif

Article Snippet: The cells were mounted with Vectashield mounting medium with DAPI (Vector Laboratories) and images were taken, using a confocal microscope (Olympus FV1000) with a × 60 oil immersion lens. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Lectin Ligand motifs AAV binding N -Acetylglucosamine (sialic acid)-binding lectins Maackia amurensis leukoagglutinin (MAL) from Maackia amurensis Neu5Ac/Gcα2,3Galβ1,4Glc(NAc) AAV1, AAV4, AAV5 Wheat germ agglutinin (WGA) from Triticum vulgaris GlcNAcβ1-4GlcNAcβ1-4GlcNAc, Neu5Ac (sialic acid) AAV4, AAV5 N -Acetylgalactosamine (galactose)-binding lectins Peanut agglutinin (PNA) from Arachis hypogaea Galβ1-3GalNAcα1-Ser/Thr AAV9 Ricinus communis agglutinin (RCA) from Ricinus communis Galβ1-4GalNAcβ1-R AAV9 Soybean agglutinin (SBA) from soybean N -Acetylgalactosamine (GalNAc) residues Mannose-binding lectins Concanavalin A (ConA) from Canavalia ensiformis α- d -Mannosyl and α- d -glucosyl residues AAV9 Fucose-binding lectins Ulex europaeus agglutinin (UEA) from Ulex europaeus Fucα1-2Gal-R Open in a separate window rAAV, recombinant adeno-associated virus.

Techniques: Binding Assay

Journal: Iranian Journal of Pathology

Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of coreE1-E2 PCR product. Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany

Article Snippet: Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene.

Techniques: Agarose Gel Electrophoresis, Marker

Journal: Iranian Journal of Pathology

Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus

doi:

Figure Lengend Snippet: Double digestion of recombinant vector by BamHI and HindIII restriction enzymes that lead to excision of core-E1-E2 fusion gene. Lane 1: digested vector and core-E1-E2 fusion fragment; Lane 2: recombinant vector linearized by BamHI; Lane M: 1kb DNA size marker (Fermentas, Germany

Article Snippet: Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene.

Techniques: Recombinant, Plasmid Preparation, Marker

Journal: Iranian Journal of Pathology

Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus

doi:

Figure Lengend Snippet: Detection of core-E1-E2 mRNA in transfected and non-transfected Huh- 7.5 cells by RT-PCR analysis. RTPCR analysis using specific primers designated for N-terminal region of fragment showed negative results in non- transfected cells (lane3) and a band with a size of approximately 950bp in transfected cells with recombinant vector (lanes 1,2). Lane M: 1kb DNA size marker

Article Snippet: Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene.

Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Recombinant, Plasmid Preparation, Marker

Journal: Methods in enzymology

Article Title: Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars

doi: 10.1016/bs.mie.2017.06.003

Figure Lengend Snippet: Plasmids used in this manuscript.

Article Snippet: Transform plasmid into freshly-competent BL21 (DE3) RIL cells, growing transformants on LB-agar selection media containing corresponding vector selection antibiotic (see ) and 30 μg/mL CAM. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Construct Vector Resistance Reference Addgene ID GST-PglFΔ1–130 ( Cj ) pGEX4T-2 Carb, 50 μg/mL Olivier et al., 2006 89708 His 8 -TEV-PglC ( Ng ) modified pET30b(+) Kan, 30 μμg/mL Hartley et al., 2011 89709 T7-PglD-His 6 ( Cj ) pET24a(+) Kan, 30 μg/mL Morrison et al., 2010 89710 PseB-His 6 ( Cj ) pET-24a(+) Kan, 30 μg/mL this report 89723 His 8 -TEV-PseC ( Cj ) modified pET30b(+) Kan, 30 μg/mL Hartley et al., 2011 89724 His 8 -TEV-PseH ( Cj ) modified pET30b(+) Kan, 30 μg/mL Hartley et al., 2011 89725 T7-WbpB-His 6 ( Pa ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2009 89711 T7-WbpE-His 6 ( Pa ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2009 89712 T7-WbpD-His 6 ( Pa ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2009 89713 T7-AglK-His 6 ( Mv ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2013 89714 T7-AglC-His 6 ( Mv ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2013 89726 Open in a separate window Plasmids used in this manuscript.

Techniques: Plasmid Preparation, Modification