Journal: Frontiers in Cellular and Infection Microbiology

Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

doi: 10.3389/fcimb.2023.1183078

Figure Lengend Snippet: Diagnostic profile of clinical images, histopathological findings, Ziehl-Neelsen staining, and DNA microarray chip assay of participants with cutaneous mycobacterial infections. (A-C) Skin lesions of participants with cutaneous infection of M. marinum (A) , M. abscessus (B) , MTB (C) , and M. chelonae (D) . (E, F) Hematoxylin and eosin staining revealing acanthosis, pseudoepitheliomatous hyperplasia or exocytosis, diffuse inflammatory cells infiltration in the dermis, including multinucleated giant cells (white arrow), lymphocytes, neutrophils, and plasmocytes (magnification: A, 40×; B, 200×). (G) Ziehl-Neelsen staining of isolates from skin tissue culture showing red club-shaped filaments. (H-K) Mapped images of the DNA microarray chip assay of skin tissue confirming infection with M. marinum (H) , M. abscessus (I) , MTB (J) , and M. chelonae (K) .

Article Snippet: The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis .

Techniques: Diagnostic Assay, Staining, Microarray, Infection

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

doi: 10.3389/fcimb.2023.1183078

Figure Lengend Snippet: Sensitivities, specificity and accuracy of the skin culture method and DNA microarray chip assay in the diagnosis of cutaneous mycobacterial infections.

Article Snippet: The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis .

Techniques: Microarray, Biomarker Discovery

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

doi: 10.3389/fcimb.2023.1183078

Figure Lengend Snippet: PLR, NLR, Youden’s index and OR of the skin tissue culture method and DNA microarray chip assay.

Article Snippet: The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis .

Techniques: Microarray

Journal: BMC Infectious Diseases

Article Title: GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

doi: 10.1186/s12879-018-3131-8

Figure Lengend Snippet: CapitalBio™ DNA microarray detection site layout. The contents of the table on the right side correspond to the microarray hybridization dot matrix on the left side in each figure. Every five repeated hybrid grid points correspond to one cell of specific content. QC: surface chemical quality control probe; EC: external control probe for hybridization-based quantitation; BC: blank control; NC: negative control probe; IC: internal control probe for PCR; WT: wild-type. a : Six sites detected in the rpoB gene, Ser531Leu (TCG → TTG), Ser531Trp (TCG → TGG), His526Asp (CAC → GAC), His526Tyr (CAC → TAC), His526Leu (CAC → CTC), His526Arg (CAC → CGC), Leu511Pro (CTG → CCG), Gln513Leu (CAA → CCA), Gln513Lys (CAA → AAA), Asp516Val (GAC → GTC), Asp516Tyr (GAC → TAC), Asp516Gly (GAC → GGC) and Leu533Pro (CTG → CCG), for a total of 13 types of mutants. b : The katG gene and a locus of the inhA gene promoter were tested as isoniazid resistance-related genes. The contents of the table on the right side correspond to the microarray hybridization dot matrix on the left side in each figure. Two katG gene mutants, Ser315Thr (AGC → ACC) and Ser315Asn (AGC → AAC), and one inhA gene promoter mutant, − 15 (C → T) mutant, were identified

Article Snippet: We used the CapitalBio TM DNA microarray method and the DST approach as the reference standard to assess these cases in Changchun for rpoB and inhA mutations.

Techniques: Microarray, Hybridization, Control, Quantitation Assay, Negative Control, Mutagenesis

Journal: BMC Infectious Diseases

Article Title: GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

doi: 10.1186/s12879-018-3131-8

Figure Lengend Snippet: Common results of the CapitalBio™ DNA microarray detection spectra are shown for samples with mutation(s) at a : WT: wild-type. b : NTB: nontuberculous mycobacteria. c : rpoB gene codon 531 (TCG → TTG). d : rpoB gene codon 526 (CAC → TAC). e : katG gene codon 315 (AGC → ACC). f : inhA gene promoter − 15 (C → T)

Article Snippet: We used the CapitalBio TM DNA microarray method and the DST approach as the reference standard to assess these cases in Changchun for rpoB and inhA mutations.

Techniques: Microarray, Mutagenesis

Journal: BMC Infectious Diseases

Article Title: GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

doi: 10.1186/s12879-018-3131-8

Figure Lengend Snippet: Performance evaluation of the CapitalBio™ DNA microarray for rifampin and isoniazid resistance in tuberculosis cases compared with the standard drug sensitivity testing (DST) method for the 671 samples

Article Snippet: We used the CapitalBio TM DNA microarray method and the DST approach as the reference standard to assess these cases in Changchun for rpoB and inhA mutations.

Techniques: Microarray

Journal: BMC Infectious Diseases

Article Title: GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

doi: 10.1186/s12879-018-3131-8

Figure Lengend Snippet: Performance evaluation of the CapitalBio™ DNA microarray for MDR-TB cases compared with the standard drug sensitivity testing (DST) method for the 671 samples

Article Snippet: We used the CapitalBio TM DNA microarray method and the DST approach as the reference standard to assess these cases in Changchun for rpoB and inhA mutations.

Techniques: Microarray

Journal: BMC Infectious Diseases

Article Title: GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

doi: 10.1186/s12879-018-3131-8

Figure Lengend Snippet: Microarray chip detection of mutations in Mycobacterium tuberculosis rpoB-RRDR relevant mutation sites for the 57 samples

Article Snippet: We used the CapitalBio TM DNA microarray method and the DST approach as the reference standard to assess these cases in Changchun for rpoB and inhA mutations.

Techniques: Microarray, Mutagenesis

Journal: BMC Infectious Diseases

Article Title: GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

doi: 10.1186/s12879-018-3131-8

Figure Lengend Snippet: Microarray chip detection of rpoB-RRDR,KatG315 and inhA-15 mutation points for the 121 samples

Article Snippet: We used the CapitalBio TM DNA microarray method and the DST approach as the reference standard to assess these cases in Changchun for rpoB and inhA mutations.

Techniques: Microarray, Mutagenesis