cal33 cells Search Results


cal33 cells  (CLS Cell Lines Service GmbH)
91
CLS Cell Lines Service GmbH cal33 cells
Figure 3. Intake of the SPIONs by the tumor cells. (a) Representative images of <t>Cal33</t> cells, an HPV-negative (HPV-) head and neck squamous cell carcinoma (HNSCC) cell line, with different concentrations of nanoparticles (0, 5, and 20 µg Fe/mL) over time (1, 24, 48, and 72 h). For the full course of the interaction and depiction of 50 µg Fe/mL, see the video in Supplementary Materials. (b) Immunofluorescence images of a cell from an HPV- HNSCC cell line RPMI 2650. Top to bottom: merge with DAPI (blue), α-Tubulin (green), nanoparticles (red); greyscale images of DAPI, α-Tubulin with orange line as estimated outline of the cell, nanoparticles captured with transmitted light microscope with red arrow on nanoparticles on cell margin, and white arrow on nanoparticles inside cell. (c) Three-dimensional immunofluorescence imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue). (d) Orthogonal imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue).
Cal33 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal33+cells/CAL-33+Cells/pm36678083-185-9-52
Average 91 stars, based on 1 article reviews
cal33 cells - by Bioz Stars, 2026-09
91/100 stars
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cal33  (JCRB Cell Bank)
90
JCRB Cell Bank cal33
Figure 3. Intake of the SPIONs by the tumor cells. (a) Representative images of <t>Cal33</t> cells, an HPV-negative (HPV-) head and neck squamous cell carcinoma (HNSCC) cell line, with different concentrations of nanoparticles (0, 5, and 20 µg Fe/mL) over time (1, 24, 48, and 72 h). For the full course of the interaction and depiction of 50 µg Fe/mL, see the video in Supplementary Materials. (b) Immunofluorescence images of a cell from an HPV- HNSCC cell line RPMI 2650. Top to bottom: merge with DAPI (blue), α-Tubulin (green), nanoparticles (red); greyscale images of DAPI, α-Tubulin with orange line as estimated outline of the cell, nanoparticles captured with transmitted light microscope with red arrow on nanoparticles on cell margin, and white arrow on nanoparticles inside cell. (c) Three-dimensional immunofluorescence imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue). (d) Orthogonal imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue).
Cal33, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal33+cells/cal33/pm40121428-42-3-11
Average 90 stars, based on 1 article reviews
cal33 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Figure 3. Intake of the SPIONs by the tumor cells. (a) Representative images of Cal33 cells, an HPV-negative (HPV-) head and neck squamous cell carcinoma (HNSCC) cell line, with different concentrations of nanoparticles (0, 5, and 20 µg Fe/mL) over time (1, 24, 48, and 72 h). For the full course of the interaction and depiction of 50 µg Fe/mL, see the video in Supplementary Materials. (b) Immunofluorescence images of a cell from an HPV- HNSCC cell line RPMI 2650. Top to bottom: merge with DAPI (blue), α-Tubulin (green), nanoparticles (red); greyscale images of DAPI, α-Tubulin with orange line as estimated outline of the cell, nanoparticles captured with transmitted light microscope with red arrow on nanoparticles on cell margin, and white arrow on nanoparticles inside cell. (c) Three-dimensional immunofluorescence imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue). (d) Orthogonal imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue).

Journal: Nanomaterials (Basel, Switzerland)

Article Title: In Vitro Analysis of Superparamagnetic Iron Oxide Nanoparticles Coated with APTES as Possible Radiosensitizers for HNSCC Cells.

doi: 10.3390/nano13020330

Figure Lengend Snippet: Figure 3. Intake of the SPIONs by the tumor cells. (a) Representative images of Cal33 cells, an HPV-negative (HPV-) head and neck squamous cell carcinoma (HNSCC) cell line, with different concentrations of nanoparticles (0, 5, and 20 µg Fe/mL) over time (1, 24, 48, and 72 h). For the full course of the interaction and depiction of 50 µg Fe/mL, see the video in Supplementary Materials. (b) Immunofluorescence images of a cell from an HPV- HNSCC cell line RPMI 2650. Top to bottom: merge with DAPI (blue), α-Tubulin (green), nanoparticles (red); greyscale images of DAPI, α-Tubulin with orange line as estimated outline of the cell, nanoparticles captured with transmitted light microscope with red arrow on nanoparticles on cell margin, and white arrow on nanoparticles inside cell. (c) Three-dimensional immunofluorescence imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue). (d) Orthogonal imaging of an RPMI 2650 cell with FITC-linked SPIONs (green), α-Tubulin (red), and DAPI (blue).

Article Snippet: Doubling times of the (c) RPMI 2650 and (f) Cal33 cells at doses from 0 to 8 Gy. (g) Exempl ry growth cu ves of cell line (BEAS-2B) with and without 20 μg Fe/mL SPIONs and at 0 and 2 Gy over 120 h. (h) Doubling times of the cell lines BEAS-2B, CLS 354, Det 562, HSC-4, UD-SCC-2, and UM-SCC-47, each with and without SPIONs at doses of 0 and 2 Gy. (i) Significances between the different groups of (h); error bars indicate the standard deviation.

Techniques: Light Microscopy, Imaging

Figure 5. Detection of cytotoxic effects of SPION-APTES via colony formation assays. (a) Representa- tive images of stained BEAS-2B (healthy bronchial epithelium cell line) colonies in Petri dishes during colony formation assay; top: control, bottom: 2 Gy of ionizing radiation and 20 µg Fe/mL of SPIONs. Logarithmic plots of survival fraction of (b) RPMI 2650 and (c) Cal33 cells; control group without SPIONs (green) and treated group with 20 µg Fe/mL SPIONs (black); ionizing radiation doses from 0 to 8 Gy; * describes significance with p = 0.05 between control group and nanoparticle group at 8 Gy, determined by Mann–Whitney U-test. Logarithmic plots of surviving fraction at 0 and 2 Gy without SPIONs (green) and with 20 µg Fe/mL of SPIONs (black); dashed lines represent nanoparticle groups normalized to 1; cell lines (d) BEAS-2B; (e) Detroit 562 (Det 562); (f) HSC-4; * describes significance with p = 0.05 between control group and nanoparticle group at 2 Gy, determined by Mann–Whitney U-test; (g) UD-SCC-2; (h) UM-SCC-47.

Journal: Nanomaterials (Basel, Switzerland)

Article Title: In Vitro Analysis of Superparamagnetic Iron Oxide Nanoparticles Coated with APTES as Possible Radiosensitizers for HNSCC Cells.

doi: 10.3390/nano13020330

Figure Lengend Snippet: Figure 5. Detection of cytotoxic effects of SPION-APTES via colony formation assays. (a) Representa- tive images of stained BEAS-2B (healthy bronchial epithelium cell line) colonies in Petri dishes during colony formation assay; top: control, bottom: 2 Gy of ionizing radiation and 20 µg Fe/mL of SPIONs. Logarithmic plots of survival fraction of (b) RPMI 2650 and (c) Cal33 cells; control group without SPIONs (green) and treated group with 20 µg Fe/mL SPIONs (black); ionizing radiation doses from 0 to 8 Gy; * describes significance with p = 0.05 between control group and nanoparticle group at 8 Gy, determined by Mann–Whitney U-test. Logarithmic plots of surviving fraction at 0 and 2 Gy without SPIONs (green) and with 20 µg Fe/mL of SPIONs (black); dashed lines represent nanoparticle groups normalized to 1; cell lines (d) BEAS-2B; (e) Detroit 562 (Det 562); (f) HSC-4; * describes significance with p = 0.05 between control group and nanoparticle group at 2 Gy, determined by Mann–Whitney U-test; (g) UD-SCC-2; (h) UM-SCC-47.

Article Snippet: Doubling times of the (c) RPMI 2650 and (f) Cal33 cells at doses from 0 to 8 Gy. (g) Exempl ry growth cu ves of cell line (BEAS-2B) with and without 20 μg Fe/mL SPIONs and at 0 and 2 Gy over 120 h. (h) Doubling times of the cell lines BEAS-2B, CLS 354, Det 562, HSC-4, UD-SCC-2, and UM-SCC-47, each with and without SPIONs at doses of 0 and 2 Gy. (i) Significances between the different groups of (h); error bars indicate the standard deviation.

Techniques: Staining, Colony Assay, Control, MANN-WHITNEY

Figure 6. Analysis of the cytostatic effect of SPIONs at 20 µg Fe/mL via growth curves through 24-well microscopy. Growth curves of (a) RPMI 2650 and (d) Cal33 cells from 0 to 8 Gy, each with and without SPIONs over 120 h; for reasons of clarity, they are only depicted at 0, 2, and 6 Gy; dashed lines show nanoparticle group. Exemplary presentation of all doses of (b) RPMI 2650 cells at 90 h and (e) Cal33 cells at 105 h (end of exponential growth). Doubling times of the (c) RPMI 2650 and (f) Cal33 cells at doses from 0 to 8 Gy. (g) Exemplary growth curves of cell line (BEAS-2B) with and without 20 µg Fe/mL SPIONs and at 0 and 2 Gy over 120 h. (h) Doubling times of the cell lines BEAS-2B, CLS 354, Det 562, HSC-4, UD-SCC-2, and UM-SCC-47, each with and without SPIONs at doses of 0 and 2 Gy. (i) Significances between the different groups of (h); error bars indicate the standard deviation. * equals significance between control group and SPION group at respective radiation dose, determined by Mann–Whitney U-test with p = 0.05.

Journal: Nanomaterials (Basel, Switzerland)

Article Title: In Vitro Analysis of Superparamagnetic Iron Oxide Nanoparticles Coated with APTES as Possible Radiosensitizers for HNSCC Cells.

doi: 10.3390/nano13020330

Figure Lengend Snippet: Figure 6. Analysis of the cytostatic effect of SPIONs at 20 µg Fe/mL via growth curves through 24-well microscopy. Growth curves of (a) RPMI 2650 and (d) Cal33 cells from 0 to 8 Gy, each with and without SPIONs over 120 h; for reasons of clarity, they are only depicted at 0, 2, and 6 Gy; dashed lines show nanoparticle group. Exemplary presentation of all doses of (b) RPMI 2650 cells at 90 h and (e) Cal33 cells at 105 h (end of exponential growth). Doubling times of the (c) RPMI 2650 and (f) Cal33 cells at doses from 0 to 8 Gy. (g) Exemplary growth curves of cell line (BEAS-2B) with and without 20 µg Fe/mL SPIONs and at 0 and 2 Gy over 120 h. (h) Doubling times of the cell lines BEAS-2B, CLS 354, Det 562, HSC-4, UD-SCC-2, and UM-SCC-47, each with and without SPIONs at doses of 0 and 2 Gy. (i) Significances between the different groups of (h); error bars indicate the standard deviation. * equals significance between control group and SPION group at respective radiation dose, determined by Mann–Whitney U-test with p = 0.05.

Article Snippet: Doubling times of the (c) RPMI 2650 and (f) Cal33 cells at doses from 0 to 8 Gy. (g) Exempl ry growth cu ves of cell line (BEAS-2B) with and without 20 μg Fe/mL SPIONs and at 0 and 2 Gy over 120 h. (h) Doubling times of the cell lines BEAS-2B, CLS 354, Det 562, HSC-4, UD-SCC-2, and UM-SCC-47, each with and without SPIONs at doses of 0 and 2 Gy. (i) Significances between the different groups of (h); error bars indicate the standard deviation.

Techniques: Microscopy, Standard Deviation, Control, MANN-WHITNEY