cal-520 am Search Results


cal 520am  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology cal 520am
Cal 520am, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/Cal-520+%2C+AM/pmc06586933-216-6-7
Average 91 stars, based on 1 article reviews
cal 520am - by Bioz Stars, 2026-10
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dishes shrna-transfected cells loaded cal-520 am  (MatTek)
90
MatTek dishes shrna-transfected cells loaded cal-520 am
A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator <t>Cal-520,</t> with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.
Dishes Shrna Transfected Cells Loaded Cal 520 Am, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/dishes+shrna+transfected+cells+loaded+cal+520+am/bio_rxiv__294611-232-7-0
Average 90 stars, based on 1 article reviews
dishes shrna-transfected cells loaded cal-520 am - by Bioz Stars, 2026-10
90/100 stars
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cal-520/am  (Biomol GmbH)
90
Biomol GmbH cal-520/am
A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator <t>Cal-520,</t> with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.
Cal 520/Am, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/cal520+am/10__1523_slash_eneuro__0223___18__2018-83-16-23
Average 90 stars, based on 1 article reviews
cal-520/am - by Bioz Stars, 2026-10
90/100 stars
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calbryte 520-am  (AAT Bioquest)
90
AAT Bioquest calbryte 520-am
A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator <t>Cal-520,</t> with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.
Calbryte 520 Am, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/cal+520+am/pmc07060274-229-9-11
Average 90 stars, based on 1 article reviews
calbryte 520-am - by Bioz Stars, 2026-10
90/100 stars
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cal-520 am dye  (MultiCell Technologies)
90
MultiCell Technologies cal-520 am dye
A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator <t>Cal-520,</t> with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.
Cal 520 Am Dye, supplied by MultiCell Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/cal+520+am+dye/pmc12194107-76-6-13
Average 90 stars, based on 1 article reviews
cal-520 am dye - by Bioz Stars, 2026-10
90/100 stars
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cal- 520 am dye  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH cal- 520 am dye
A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator <t>Cal-520,</t> with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.
Cal 520 Am Dye, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/cal++520+am+dye/pm37406102-236-7-11
Average 90 stars, based on 1 article reviews
cal- 520 am dye - by Bioz Stars, 2026-10
90/100 stars
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cal-520 am  (Cosmo Bio USA)
90
Cosmo Bio USA cal-520 am
A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator <t>Cal-520,</t> with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.
Cal 520 Am, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cal-520+am/cal+520+am/pm23806689-60-0-5
Average 90 stars, based on 1 article reviews
cal-520 am - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator Cal-520, with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.

Journal: bioRxiv

Article Title: Myosin-II mediated traction forces evoke localized Piezo1 Ca 2+ flickers

doi: 10.1101/294611

Figure Lengend Snippet: A. Schematic of the Molecular Tension Sensor (MTS) used for traction force imaging. The N-terminal region of the sensor (blue) is tethered to a PEG-functionalized glass coverslip. A fibronectin domain at the C-terminal end (brown) binds to the cell’s integrins (purple), allowing cells to attach to the glass coverslip. An elastic spring domain bridges the two ends of the sensor and separates a FRET donor (green) and acceptor (red). Cell-generated traction forces pull the FRET pair apart, resulting in reduced FRET efficiency. The FRET index (the ratio of acceptor intensity over summed donor and acceptor intensities), serves as a measure of force; with a low FRET index indicating high force, and a high FRET index indicating a low force. B. Imaging of Piezo1 Ca 2+ flickers. Panels show resting fluorescence of HFF cells loaded with Ca 2+ indicator Cal-520, with overlaid red dots marking the centroid locations of Ca 2+ flickers. C. Corresponding force maps from the same cells, overlaid with red dots marking the Ca 2+ flicker locations. Blue denotes low FRET (high force) and red denotes high FRET (low force). The color bar in C represents FRET efficiency (top) and the average force per MTS per pixel in pN obtained from calibrated FRET-Force curves of the MTS as described in Methods. D. Box and whisker plots with red boxes showing distances from Piezo1 flicker localizations to the nearest traction force region for HFFs (top: 515 flickers from 9 cells) and hNSPCs (bottom: 66 flickers from 18 cells). Grey boxes show corresponding mean distances derived from simulations of 9000 random intracellular locations for each cell. Box range is 25 th to 75 th percentile; whiskers denote 10 th and 90 th percentile, horizontal lines represent the median and filled black squares represent mean. *** denotes p < 0.001 by Kolmogorov-Smirnov test.

Article Snippet: Mat-tek dishes of shRNA-transfected cells loaded with Cal-520 AM were first scanned using an Olympus UPLSAPO 10× objective to identify cells expressing TurboRFP.

Techniques: Imaging, Generated, Fluorescence, Whisker Assay, Derivative Assay